CTHRSSVVC peptide as a possible early molecular imaging target for atherosclerosis

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dc.contributor.authorSILVA, ROSEMEIRE A.
dc.contributor.authorGIORDANO, RICARDO J.
dc.contributor.authorGUTIERREZ, PAULO S.
dc.contributor.authorROCHA, VIVIANE Z.
dc.contributor.authorRUDNICKI, MARTINA
dc.contributor.authorKEE, PATRICK
dc.contributor.authorABDALLA, DULCINEIA S.P.
dc.contributor.authorPUECH-LEAO, PEDRO
dc.contributor.authorCARAMELLI, BRUNO
dc.contributor.authorARAP, WADIH
dc.contributor.authorPASQUALINI, RENATA
dc.contributor.authorMENEGHETTI, JOSE C.
dc.contributor.authorMARQUES, FABIO L.N.
dc.contributor.authorKHOOBCHANDANI, MENKA
dc.contributor.authorKATTI, KATTESH V.
dc.contributor.authorLUGAO, ADEMAR B.
dc.contributor.authorKALIL, JORGE
dc.coverageInternacionalpt_BR
dc.date.accessioned2017-03-14T16:17:52Z
dc.date.available2017-03-14T16:17:52Z
dc.date.issued2016pt_BR
dc.description.abstractThe purpose of our work was to select phages displaying peptides capable of binding to vascular markers present in human atheroma, and validate their capacity to target the vascular markers in vitro and in low-density lipoprotein receptor knockout (LDLr􀀀/􀀀) mouse model of atherosclerosis. By peptide fingerprinting on human atherosclerotic tissues, we selected and isolated four different peptides sequences, which bind to atherosclerotic lesions and share significant similarity to known human proteins with prominent roles in atherosclerosis. The CTHRSSVVC-phage peptide displayed the strongest reactivity with human carotid atherosclerotic lesions (p < 0.05), when compared to tissues from normal carotid arteries. This peptide sequence shares similarity to a sequence present in the fifth scavenger receptor cysteine-rich (SRCR) domain of CD163, which appeared to bind to CD163, and subsequently, was internalized by macrophages. Moreover, the CTHRSSVVC-phage targets atherosclerotic lesions of a low-density lipoprotein receptor knockout (LDLr􀀀/􀀀) mouse model of atherosclerosis in vivo to High-Fat diet group versus Control group. Tetraazacyclododecane-1,4,7,10-tetraacetic acid-CTHRSSVVC peptide (DOTA-CTHRSSVVC) was synthesized and labeled with 111InCl3 in >95% yield as determined by high performance liquid chromatography (HPLC), to validate the binding of the peptide in atherosclerotic plaque specimens. The results supported our hypothesis that CTHRSSVVC peptide has a remarkable sequence for the development of theranostics approaches in the treatment of atherosclerosis and other diseases.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipIDFAPESP: 04/02721-8pt_BR
dc.format.extent1-18pt_BR
dc.identifier.citationSILVA, ROSEMEIRE A.; GIORDANO, RICARDO J.; GUTIERREZ, PAULO S.; ROCHA, VIVIANE Z.; RUDNICKI, MARTINA; KEE, PATRICK; ABDALLA, DULCINEIA S.P.; PUECH-LEAO, PEDRO; CARAMELLI, BRUNO; ARAP, WADIH; PASQUALINI, RENATA; MENEGHETTI, JOSE C.; MARQUES, FABIO L.N.; KHOOBCHANDANI, MENKA; KATTI, KATTESH V.; LUGAO, ADEMAR B.; KALIL, JORGE. CTHRSSVVC peptide as a possible early molecular imaging target for atherosclerosis. <b>International Journal of Molecular Sciences</b>, v. 17, n. 9, p. 1-18, 2016. DOI: <a href="https://dx.doi.org/10.3390/ijms17091383">10.3390/ijms17091383</a>. Disponível em: http://repositorio.ipen.br/handle/123456789/27163.
dc.identifier.doi10.3390/ijms17091383pt_BR
dc.identifier.fasciculo9pt_BR
dc.identifier.issn1661-6596pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-1737-3191
dc.identifier.percentilfi63.80
dc.identifier.urihttp://repositorio.ipen.br/handle/123456789/27163
dc.identifier.vol17pt_BR
dc.relation.ispartofInternational Journal of Molecular Sciencespt_BR
dc.rightsopenAccesspt_BR
dc.subjectarteriosclerosis
dc.subjectpeptides
dc.subjectmacrophages
dc.subjectproteins
dc.subjecttheranostics
dc.titleCTHRSSVVC peptide as a possible early molecular imaging target for atherosclerosispt_BR
dc.typeArtigo de periódicopt_BR
dspace.entity.typePublication
ipen.autorADEMAR BENEVOLO LUGAO
ipen.codigoautor339
ipen.contributor.ipenauthorADEMAR BENEVOLO LUGAO
ipen.date.recebimento17-03pt_BR
ipen.identifier.fi3.226pt_BR
ipen.identifier.ipendoc23506pt_BR
ipen.identifier.iwosWoSpt_BR
ipen.identifier.ods3
ipen.range.fi3.000 - 4.499
ipen.range.percentilfi50.00 - 74.99
ipen.type.genreArtigo
relation.isAuthorOfPublication99ac24c5-2ae1-465a-a6f2-40b4d9af6af7
relation.isAuthorOfPublication.latestForDiscovery99ac24c5-2ae1-465a-a6f2-40b4d9af6af7
sigepi.autor.atividadeLUGAO, ADEMAR B.:339:740:Npt_BR
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