Characterization and evaluation of the enzymatic activity of tetanus toxin submitted to cobalt-60 gamma radiation

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dc.contributor.authorSARTORI, GISELLE P.pt_BR
dc.contributor.authorCOSTA, ANDREA dapt_BR
dc.contributor.authorMACARINI, FERNANDO L. dos S.pt_BR
dc.contributor.authorMARIANO, DOUGLAS O.C.pt_BR
dc.contributor.authorPIMENTA, DANIEL C.pt_BR
dc.contributor.authorSPENCER, PATRICK J.pt_BR
dc.contributor.authorNALI, LUIZ H. da S.pt_BR
dc.contributor.authorGALISTEO JUNIOR, ANDRES J.pt_BR
dc.coverageInternacionalpt_BR
dc.date.accessioned2021-07-23T16:38:57Z
dc.date.available2021-07-23T16:38:57Z
dc.date.issued2021pt_BR
dc.description.abstractBackground Tetanus toxin blocks the release of the inhibitory neurotransmitters in the central nervous system and causes tetanus and its main form of prevention is through vaccination. The vaccine is produced by inactivation of tetanus toxin with formaldehyde, which may cause side effects. An alternative way is the use of ionizing radiation for inactivation of the toxin and also to improve the potential immunogenic response and to reduce the post-vaccination side effects. Therefore, the aim of this study was to characterize the tetanus toxin structure after different doses of ionizing radiation of 60Co. Methods Irradiated and native tetanus toxin was characterized by SDS PAGE in reducing and non-reducing conditions and MALD-TOF. Enzymatic activity was measured by FRET substrate. Also, antigenic properties were assessed by ELISA and Western Blot data. Results Characterization analysis revealed gradual modification on the tetanus toxin structure according to doses increase. Also, fragmentation and possible aggregations of the protein fragments were observed in higher doses. In the analysis of peptide preservation by enzymatic digestion and mass spectrometry, there was a slight modification in the identification up to the dose of 4 kGy. At subsequent doses, peptide identification was minimal. The analysis of the enzymatic activity by fluorescence showed 35 % attenuation in the activity even at higher doses. In the antigenic evaluation, anti-tetanus toxin antibodies were detected against the irradiated toxins at the different doses, with a gradual decrease as the dose increased, but remaining at satisfactory levels. Conclusion Ionizing radiation promoted structural changes in the tetanus toxin such as fragmentation and/or aggregation and attenuation of enzymatic activity as the dose increased, but antigenic recognition of the toxin remained at good levels indicating its possible use as an immunogen. However, studies of enzymatic activity of tetanus toxin irradiated with doses above 8 kGy should be further analyzed.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipFinanciadora de Estudos e Projetos (FINEP)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorshipIDFAPESP: 14/26782-8pt_BR
dc.description.sponsorshipIDFINEP: 01.09.0278.04; 01.12.0450.03pt_BR
dc.description.sponsorshipIDCNPq: 301974/2019-5pt_BR
dc.format.extent1-13pt_BR
dc.identifier.citationSARTORI, GISELLE P.; COSTA, ANDREA da; MACARINI, FERNANDO L. dos S.; MARIANO, DOUGLAS O.C.; PIMENTA, DANIEL C.; SPENCER, PATRICK J.; NALI, LUIZ H. da S.; GALISTEO JUNIOR, ANDRES J. Characterization and evaluation of the enzymatic activity of tetanus toxin submitted to cobalt-60 gamma radiation. <b>Journal of Venomous Animals and Toxins including Tropical Diseases</b>, v. 27, p. 1-13, 2021. DOI: <a href="https://dx.doi.org/10.1590/1678-9199-JVATITD-2020-0140">10.1590/1678-9199-JVATITD-2020-0140</a>. Disponível em: http://repositorio.ipen.br/handle/123456789/32051.
dc.identifier.doi10.1590/1678-9199-JVATITD-2020-0140pt_BR
dc.identifier.issn1678-9199pt_BR
dc.identifier.orcid0000-0001-8949-7735pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-8949-7735
dc.identifier.percentilfi29.59pt_BR
dc.identifier.percentilfiCiteScore57.25
dc.identifier.urihttp://repositorio.ipen.br/handle/123456789/32051
dc.identifier.vol27pt_BR
dc.relation.ispartofJournal of Venomous Animals and Toxins including Tropical Diseasespt_BR
dc.rightsopenAccesspt_BR
dc.subjectcobalt 60
dc.subjectdetoxification
dc.subjectenzyme activity
dc.subjectgamma radiation
dc.subjectirradiation
dc.subjectneurology
dc.subjectradiation dose units
dc.subjecttetanus
dc.subjecttoxins
dc.subjectvaccines
dc.titleCharacterization and evaluation of the enzymatic activity of tetanus toxin submitted to cobalt-60 gamma radiationpt_BR
dc.typeArtigo de periódicopt_BR
dspace.entity.typePublication
ipen.autorPATRICK JACK SPENCER
ipen.codigoautor910
ipen.contributor.ipenauthorPATRICK JACK SPENCER
ipen.date.recebimento21-07
ipen.identifier.fi2.293pt_BR
ipen.identifier.fiCiteScore3.9
ipen.identifier.ipendoc27822pt_BR
ipen.identifier.iwosWoSpt_BR
ipen.identifier.ods3
ipen.range.fi1.500 - 2.999
ipen.range.percentilfi25.00 - 49.99
ipen.type.genreArtigo
relation.isAuthorOfPublication4eb7939e-aeea-4991-8525-b3d05ac27364
relation.isAuthorOfPublication.latestForDiscovery4eb7939e-aeea-4991-8525-b3d05ac27364
sigepi.autor.atividadeSPENCER, PATRICK J.:910:810:Npt_BR
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