Evaluation of radiation-induced genotoxicity on human melanoma cells (SK-MEL-37) by flow cytometry

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dc.contributor.authorBONFIM, LETICIA
dc.contributor.authorCARVALHO, LUMA R. de
dc.contributor.authorVIEIRA, DANIEL P.
dc.coverageInternacionalpt_BR
dc.creator.eventoINTERNATIONAL NUCLEAR ATLANTIC CONFERENCEpt_BR
dc.date.accessioned2018-01-11T11:07:14Z
dc.date.available2018-01-11T11:07:14Z
dc.date.eventoOctober 22-27, 2017pt_BR
dc.description.abstractMicronucleus assay is a test used to evaluate genotoxic damage in cells, which can be caused by various factors, like ionizing radiation. Interactions between radiation energies and DNA can cause breakage, leading to use chromosomal mutations or loss of genetic material, important events that could be induced in solid tumors to mitigate its expansion within human body. Melanoma has been described as a tumor with increased radio resistance. This work evaluated micronuclei percentages (%MN) in human melanoma cells (SK-MEL-37), irradiated by gamma radiation, with doses between 0 and 16Gy. Cell suspensions were irradiated in PBS by a 60Co source in doses between 0 and 16Gy, and incubated by 48h. Then cell membranes were lysed in the presence of SYTOX Green and EMA dyes, preserving nuclear membranes. Using this method, EMA-stained nuclei could be discriminated as those derived from dead cells, and SYTOX nuclei and micronuclei could be quantified. Micronuclei percentages were found to be proportional to dose, (R2 = 0.997). Only the highest dose (16Gy) could induce statistically significant increase of MN (p<0.0001), although cultures irradiated by 4, 8 and 16Gy showed significant increase of dead cell fractions. Calculation of the nuclei-to-beads ratio showed that 8 and 16Gy could reduce melanoma cell proliferation. Results showed that although cell death and loss of proliferative capacity could be observed on cultures irradiated at lower doses, genotoxic damage could be induced only on a higher dose. Resistance to radiation-induced genotoxicity could explain a relatively high radio resistance of melanoma tumors.pt_BR
dc.event.siglaINACpt_BR
dc.identifier.citationBONFIM, LETICIA; CARVALHO, LUMA R. de; VIEIRA, DANIEL P. Evaluation of radiation-induced genotoxicity on human melanoma cells (SK-MEL-37) by flow cytometry. In: INTERNATIONAL NUCLEAR ATLANTIC CONFERENCE, October 22-27, 2017, Belo Horizonte, MG. <b>Proceedings...</b> Rio de Janeiro, RJ: Associação Brasileira de Energia Nuclear, 2017. Disponível em: http://repositorio.ipen.br/handle/123456789/28283.
dc.identifier.orcidhttps://orcid.org/0000-0002-0007-534X
dc.identifier.urihttp://repositorio.ipen.br/handle/123456789/28283
dc.localRio de Janeiro, RJpt_BR
dc.local.eventoBelo Horizonte, MGpt_BR
dc.publisherAssociação Brasileira de Energia Nuclearpt_BR
dc.rightsopenAccesspt_BR
dc.subjectanimal cells
dc.subjectcell flow systems
dc.subjectcell nuclei
dc.subjectcobalt 60
dc.subjectlymphokines
dc.subjectmelanomas
dc.subjectradiation doses
dc.subjectradiation effects
dc.subjectradiosensitivity
dc.subjectsurvival curves
dc.titleEvaluation of radiation-induced genotoxicity on human melanoma cells (SK-MEL-37) by flow cytometrypt_BR
dc.typeTexto completo de eventopt_BR
dspace.entity.typePublication
ipen.autorLUMA RAMIREZ DE CARVALHO
ipen.autorDANIEL PEREZ VIEIRA
ipen.autorLETICIA BONFIM
ipen.codigoautor12133
ipen.codigoautor3158
ipen.codigoautor14185
ipen.contributor.ipenauthorLUMA RAMIREZ DE CARVALHO
ipen.contributor.ipenauthorDANIEL PEREZ VIEIRA
ipen.contributor.ipenauthorLETICIA BONFIM
ipen.date.recebimento18-01pt_BR
ipen.event.datapadronizada2017pt_BR
ipen.identifier.ipendoc24108pt_BR
ipen.notas.internasProceedingspt_BR
ipen.type.genreArtigo
relation.isAuthorOfPublication8e39b9fc-86ca-4b06-bf2a-cb8f163165d8
relation.isAuthorOfPublicationc2352608-be9c-4a73-be8c-571f10bb53d2
relation.isAuthorOfPublication4cddf0c8-9b2f-4ec1-9621-8fbf918f0b3f
relation.isAuthorOfPublication.latestForDiscovery4cddf0c8-9b2f-4ec1-9621-8fbf918f0b3f
sigepi.autor.atividadeBONFIM, LETICIA:14185:810:Spt_BR
sigepi.autor.atividadeCARVALHO, LUMA R. DE:12133:810:Npt_BR
sigepi.autor.atividadeVIEIRA, DANIEL P.:3158:810:Npt_BR
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