Efficient non-invasive plasmid-DNA administration into tibialis cranialis muscle of “little” mice
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2017
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Current Molecular Medicine
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Background: An alternative treatment for growth hormone deficiency based
on hGH-DNA administration, followed by electro gene transfer, was investigated by
injecting the plasmid into surgically exposed or non-exposed quadriceps or tibialis
muscle of immunodeficient “little” mice.
Methods: An optimization of electrotransfer conditions via a new combination of high/low
voltage pulses is presented. After 3 days, serum hGH was determined and in a 28-day
assay, the relative growth parameters were compared.
Results: Both groups exhibited similar results: 5.0 ± 2.2 (SD) and 3.5 ± 0.9 ng hGH/ml
(P>0.05; n=7) for the exposed quadriceps and non-exposed tibialis treatments,
respectively. The final body weight increases were 16.1% for the quadriceps and 18.9%
for the tibialis group. The tail and nose-to-tail length increases were 4.5% and 7.1% for
the quadriceps and 4.8 and 4.6% for the tibialis group. The right and left femur length
increases, obtained from radiographic measurements, were 16.9% and 12.7% for the
quadriceps and 19.4% and 12.3% for the tibialis, respectively. A non-significant
difference between exposed quadriceps and non-exposed tibialis treatments (P=0.48)
was confirmed via a completely integrated statistical analysis. Circulating mIGF-1 levels
were 126 ± 47, 106 ± 93 (P>0.05) and 38 ± 15 ng/ml for the quadriceps, tibialis and
saline treatments, respectively.
Conclusion: These results show that hGH-DNA administration into non-exposed tibialis
muscle followed by the new HV/LV electrotransfer protocol was an equally efficient, less
traumatic treatment,
Como referenciar
CECCHI, C.R.; HIGUTI, E.; LIMA, E.R.; VIEIRA, D.P.; SQUAIR, P.L.; PERONI, C.N.; BARTOLINI, P. Efficient non-invasive plasmid-DNA administration into tibialis cranialis muscle of “little” mice. Current Molecular Medicine, v. 17, n. 3, p. 230-235, 2017. DOI: 10.2174/1566524017666170721152119. Disponível em: http://repositorio.ipen.br/handle/123456789/28442. Acesso em: 13 May 2024.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.