Exploring the effects of low-level laser therapy on fibroblasts and tumor cells following gamma radiation exposure

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dc.contributor.authorSILVA, CAMILA R.
dc.contributor.authorCABRAL, FERNANDA V.
dc.contributor.authorCAMARGO, CLAUDINEI F.M. de
dc.contributor.authorNUNEZ, SILVIA C.
dc.contributor.authorYOSHIMURA, TANIA M.
dc.contributor.authorLUNA, ARTHUR C. de L.
dc.contributor.authorMARIA, DURVANEI A.
dc.contributor.authorRIBEIRO, MARTHA S.
dc.coverageInternacionalpt_BR
dc.date.accessioned2017-03-17T13:31:49Z
dc.date.available2017-03-17T13:31:49Z
dc.date.issued2016pt_BR
dc.description.abstractIonizing radiation (IR) induces DNA damage and low-level laser therapy (LLLT) has been investigated to prevent or repair detrimental outcomes resulting from IR exposure. Few in vitro studies, however, explore the biological mechanisms underlying those LLLT benefits. Thus, in this work, fibroblasts and tumor cells are submitted to IR with doses of 2.5 Gy and 10 Gy. After twenty-four-h, the cells are exposed to LLLT with fluences of 30 J cm–2, 90 J cm–2, and 150 J cm–2. Cellular viability, cell cycle phases, cell proliferation index and senescence are evaluated on days 1 and 4 after LLLT irradiation. For fibroblasts, LLLT promotes – in a fluence-dependent manner – increments in cell viability and proliferation, while a reduction in the senescence was observed. Regarding tumor cells, no influences of LLLT on cell viability are noticed. Whereas LLLT enhances cell populations in S and G2/M cell cycle phases for both cellular lines, a decrease in proliferation and increase in senescence was verified only for tumor cells. Putting together, the results suggest that fibroblasts and tumor cells present different responses to LLLT following exposure to gamma-radiation, and these promising results should stimulate further investigations. Senescence of tumor cells and fibroblasts on the 4th day after ionizing radiation (IR) and low-level laser therapy (LLLT) exposures. The number of senescent cells increased significantly for tumor cells (a) while for fibroblasts no increment was observed (b). The blue collor indicates senescence activity.pt_BR
dc.format.extent1157-1166pt_BR
dc.identifier.citationSILVA, CAMILA R.; CABRAL, FERNANDA V.; CAMARGO, CLAUDINEI F.M. de; NUNEZ, SILVIA C.; YOSHIMURA, TANIA M.; LUNA, ARTHUR C. de L.; MARIA, DURVANEI A.; RIBEIRO, MARTHA S. Exploring the effects of low-level laser therapy on fibroblasts and tumor cells following gamma radiation exposure. <b>Journal of Biophotonics</b>, v. 9, n. 11-12, p. 1157-1166, 2016. DOI: <a href="https://dx.doi.org/10.1002/jbio.201600107">10.1002/jbio.201600107</a>. Disponível em: http://repositorio.ipen.br/handle/123456789/27214.
dc.identifier.doi10.1002/jbio.201600107pt_BR
dc.identifier.fasciculo11-12pt_BR
dc.identifier.issn1864-063Xpt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-4203-1134
dc.identifier.percentilfi84.66
dc.identifier.urihttp://repositorio.ipen.br/handle/123456789/27214
dc.identifier.vol9pt_BR
dc.relation.ispartofJournal of Biophotonicspt_BR
dc.rightsclosedAccesspt_BR
dc.subjectgamma radiation
dc.subjecttumor cells
dc.subjectionizing radiations
dc.subjectlaser radiation
dc.subjectmammary glands
dc.subjectneoplasms
dc.subjecttumor cells
dc.subjecttherapy
dc.subjectradiotherapy
dc.subjectlevels
dc.titleExploring the effects of low-level laser therapy on fibroblasts and tumor cells following gamma radiation exposurept_BR
dc.typeArtigo de periódicopt_BR
dspace.entity.typePublication
ipen.autorFERNANDA VIANA CABRAL
ipen.autorTANIA MATEUS YOSHIMURA
ipen.autorMARTHA SIMOES RIBEIRO
ipen.autorSILVIA CRISTINA NUNEZ
ipen.autorCLAUDINEI FRANCISCO MORAES DE CAMARGO
ipen.autorCAMILA RAMOS SILVA
ipen.codigoautor12732
ipen.codigoautor10230
ipen.codigoautor574
ipen.codigoautor3759
ipen.codigoautor11723
ipen.codigoautor11642
ipen.contributor.ipenauthorFERNANDA VIANA CABRAL
ipen.contributor.ipenauthorTANIA MATEUS YOSHIMURA
ipen.contributor.ipenauthorMARTHA SIMOES RIBEIRO
ipen.contributor.ipenauthorSILVIA CRISTINA NUNEZ
ipen.contributor.ipenauthorCLAUDINEI FRANCISCO MORAES DE CAMARGO
ipen.contributor.ipenauthorCAMILA RAMOS SILVA
ipen.date.recebimento17-03pt_BR
ipen.identifier.fi4.328pt_BR
ipen.identifier.ipendoc23565pt_BR
ipen.identifier.iwosWoSpt_BR
ipen.range.fi3.000 - 4.499
ipen.range.percentilfi75.00 - 100.00
ipen.type.genreArtigo
relation.isAuthorOfPublication622f5d85-e9c4-40d7-a55f-873e2a346f55
relation.isAuthorOfPublication36384aec-6adf-42ef-99ae-68f62b9a69b9
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relation.isAuthorOfPublication.latestForDiscovery7b89c9af-427d-4f2a-bb3d-1f6252c548c2
sigepi.autor.atividadeSILVA, CAMILA R.:11642:-1:Spt_BR
sigepi.autor.atividadeCABRAL, FERNANDA V.:12732:-1:Npt_BR
sigepi.autor.atividadeCAMARGO, CLAUDINEI F.M. DE:11723:-1:Npt_BR
sigepi.autor.atividadeNUNEZ, SILVIA C.:3759:-1:Npt_BR
sigepi.autor.atividadeYOSHIMURA, TANIA M.:10230:-1:Npt_BR
sigepi.autor.atividadeRIBEIRO, MARTHA S.:574:-1:Npt_BR
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