A mechanistic approach towards the formation of bityrosine in proteins by ionizing radiation

Página do item simplificado
dc.contributor.authorSOWINSKI, SEBASTIANpt_BR
dc.contributor.authorVARCA, GUSTAVO H.C.pt_BR
dc.contributor.authorKADLUBOWSKI, SLAWOMIRpt_BR
dc.contributor.authorLUGAO, ADEMAR B.pt_BR
dc.contributor.authorULANSKI, PIOTRpt_BR
dc.coverageInternacionalpt_BR
dc.date.accessioned2021-12-10T14:04:50Z
dc.date.available2021-12-10T14:04:50Z
dc.date.issued2021pt_BR
dc.description.abstractOne of the methods of protein crosslinking used in the synthesis of protein-based nanoparticles is the formation of bityrosine bridges, which may be achieved by the recombination of phenoxyl-type radicals in irradiated protein solutions. Radiation-induced formation of phenoxyl radicals in tyrosine is promoted in presence of H+. In this work, kinetics of this process and the influence of pH were studied by pulse radiolysis measurements of a model peptide H-Gly-Tyr-Gly-OH (GYG), which may help to solve questions raised for bigger, more complex systems in comparable conditions. The main route of phenoxyl radicals formation consists of the addition reaction of hydroxyl radical to the phenol ring on the tyrosine side-chain and proton catalyzed water molecule elimination. A similar effect was observed in studies of tripeptide solutions containing phosphate buffer. The presented data include time-resolved optical spectroscopy of transient species formed under pulse electron beam irradiation and a comparison of the kinetics of the phenoxyl radicals formation in samples at various pH and in presence of phosphate buffer. The postulated mechanism and obtained values of rate constants of the formation and decay of transient species were additionally checked by simple probabilistic simulations.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipInternational Atomic Energy Agency (IAEA)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorshipIDFAPESP: 10/10935-9; 15/13979-0pt_BR
dc.description.sponsorshipIDIAEA: CRP F22064pt_BR
dc.description.sponsorshipIDCNPq: 402887/2013-1pt_BR
dc.format.extent1-8pt_BR
dc.identifier.citationSOWINSKI, SEBASTIAN; VARCA, GUSTAVO H.C.; KADLUBOWSKI, SLAWOMIR; LUGAO, ADEMAR B.; ULANSKI, PIOTR. A mechanistic approach towards the formation of bityrosine in proteins by ionizing radiation: GYG model peptide. <b>Radiation Physics and Chemistry</b>, v. 188, p. 1-8, 2021. DOI: <a href="https://dx.doi.org/10.1016/j.radphyschem.2021.109644">10.1016/j.radphyschem.2021.109644</a>. Disponível em: http://repositorio.ipen.br/handle/123456789/32383.
dc.identifier.doi10.1016/j.radphyschem.2021.109644pt_BR
dc.identifier.issn0969-806Xpt_BR
dc.identifier.orcid0000-0002-1737-3191pt_BR
dc.identifier.orcid0000-0002-6545-237Xpt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-1737-3191
dc.identifier.percentilfi57.09pt_BR
dc.identifier.percentilfiCiteScore70.00pt_BR
dc.identifier.urihttp://repositorio.ipen.br/handle/123456789/32383
dc.identifier.vol188pt_BR
dc.relation.ispartofRadiation Physics and Chemistrypt_BR
dc.rightsopenAccesspt_BR
dc.subjectionizing radiations
dc.subjectradiolysis
dc.subjecttyrosine
dc.subjectproteins
dc.titleA mechanistic approach towards the formation of bityrosine in proteins by ionizing radiationpt_BR
dc.typeArtigo de periódicopt_BR
dspace.entity.typePublication
ipen.autorADEMAR BENEVOLO LUGAO
ipen.autorGUSTAVO HENRIQUE COSTAVARCA
ipen.codigoautor339
ipen.codigoautor8661
ipen.contributor.ipenauthorADEMAR BENEVOLO LUGAO
ipen.contributor.ipenauthorGUSTAVO HENRIQUE COSTAVARCA
ipen.date.recebimento21-12
ipen.identifier.fi2.776pt_BR
ipen.identifier.fiCiteScore4.5pt_BR
ipen.identifier.ipendoc28151pt_BR
ipen.identifier.iwosWoSpt_BR
ipen.range.fi1.500 - 2.999
ipen.range.percentilfi50.00 - 74.99
ipen.subtituloGYG model peptidept_BR
ipen.type.genreArtigo
relation.isAuthorOfPublication99ac24c5-2ae1-465a-a6f2-40b4d9af6af7
relation.isAuthorOfPublication46f998cf-b207-4854-94c0-dd2def4eec2a
relation.isAuthorOfPublication.latestForDiscovery46f998cf-b207-4854-94c0-dd2def4eec2a
sigepi.autor.atividadeLUGAO, ADEMAR B.:339:740:Npt_BR
sigepi.autor.atividadeVARCA, GUSTAVO H.C.:8661:740:Npt_BR
Pacote Original
Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
28151.pdf
Tamanho:
3.75 MB
Formato:
Adobe Portable Document Format
Descrição:
Baixar
Licença do Pacote
Agora exibindo 1 - 1 de 1
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Baixar
Coleções
Periódicos