Hepatic bioavailability of Total Mercury (THg) in Cichla sp from Tapajós River Region, Brazilian Amazon
| dc.contributor.author | AZEVEDO, J.D. | |
| dc.contributor.author | SARKIS, J.E. | |
| dc.contributor.author | HORTELLANI, M.A. | |
| dc.coverage | Internacional | pt_BR |
| dc.creator.evento | ANNUAL MEETING OF THE SOCIETY OF ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY, 24th | pt_BR |
| dc.date.accessioned | 2017-07-10T13:58:53Z | |
| dc.date.available | 2017-07-10T13:58:53Z | |
| dc.date.evento | May 11-15, 2014 | pt_BR |
| dc.description.abstract | Most of the Hg occurring in the Amazon is of natural origin. However, the intense gold mining activity in the region has increased the levels of this toxic metal, especially in the middle section of the Tapajós River. Specimens of the Cichla genus have great economic and ecological significance, as they are the most consumed fish in the Brazilian Amazon region and are at the top of the Amazon food chain. In addition, they are a good indicator of Hg bioaccumulation because the concentrations of Hg in their tissues tend to reflect the accumulation process of successive exposure. A total of 26 adult specimens of Cichla sp were obtained by fisheries in the Tapajós River region’s contaminated gold mining area. In the laboratory, morphometric data of each fish was taken, for instance total length, total weight and sex. The liver tissue was removed from each specimen, washed with distilled water, packed in polyethylene identified bags and kept at - 20ºC for subsequent total mercury (THg) analysis. Assays were carry out in the total liver and in the cytosol hepatic fraction obtained by ultracentrifugation of the tissue in buffer Tris-HCl 10 mM, pH 7.4. The analyses were performed by flow injection Cold Vapor Atomic Absorption Spectrometry (FIA-CV AAS) with detection (DL) and quantification limits (QL) of 0.527 and 2.585 μg Kg-1, respectively. Individuals of C. sp had active gonadal maturation, including spawning and post-spawning individuals. The average length and total weight was 395 ± 51 mm and 840 ± 418 g, respectively. High levels of THg were obtained in the total liver ranging from 1.7 mg Kg-1 to 3.8 mg Kg-1. However, only 0.2% of the THg were available to the cytosol hepatic fraction. A significant and positive correlation (rs = 0.65) was found between the concentration of the THg in the total tissue and in the hepatic cytosol fraction. These data are a preliminary study with respect to the bioavailability of total mercury (THg) in different compartments of the hepatic tissue of C. sp in order to understand some aspects of the detoxification mechanism of this toxic metal in bioindicator species from the Amazon region. In fact, it was possible to observe differences in the transference of this important toxic metal into the cell and its linkage in the compartments. | pt_BR |
| dc.event.sigla | SETAC | pt_BR |
| dc.format.extent | 360-361 | pt_BR |
| dc.identifier.citation | AZEVEDO, J.D.; SARKIS, J.E.; HORTELLANI, M.A. Hepatic bioavailability of Total Mercury (THg) in Cichla sp from Tapajós River Region, Brazilian Amazon. In: ANNUAL MEETING OF THE SOCIETY OF ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY, 24th, May 11-15, 2014, Basel, Switzerland. <b>Abstract...</b> p. 360-361. Disponível em: http://repositorio.ipen.br/handle/123456789/27656. | |
| dc.identifier.orcid | https://orcid.org/0000-0002-6745-8185 | |
| dc.identifier.uri | http://repositorio.ipen.br/handle/123456789/27656 | |
| dc.local.evento | Basel, Switzerland | pt_BR |
| dc.rights | openAccess | pt_BR |
| dc.title | Hepatic bioavailability of Total Mercury (THg) in Cichla sp from Tapajós River Region, Brazilian Amazon | pt_BR |
| dc.type | Resumo de eventos científicos | pt_BR |
| dspace.entity.type | Publication | |
| ipen.autor | MARCOS ANTONIO HORTELLANI | |
| ipen.autor | JORGE EDUARDO DE SOUZA SARKIS | |
| ipen.codigoautor | 713 | |
| ipen.codigoautor | 1080 | |
| ipen.contributor.ipenauthor | MARCOS ANTONIO HORTELLANI | |
| ipen.contributor.ipenauthor | JORGE EDUARDO DE SOUZA SARKIS | |
| ipen.date.recebimento | 17-07 | pt_BR |
| ipen.event.datapadronizada | 2014 | pt_BR |
| ipen.identifier.ipendoc | 23892 | pt_BR |
| ipen.notas.internas | Abstract | pt_BR |
| ipen.type.genre | Resumo | |
| relation.isAuthorOfPublication | 0110d7de-46bf-46f4-9cca-2e71256b6dae | |
| relation.isAuthorOfPublication | ad66e386-1f88-4fcc-b1d9-04bff1556e8e | |
| relation.isAuthorOfPublication.latestForDiscovery | ad66e386-1f88-4fcc-b1d9-04bff1556e8e | |
| sigepi.autor.atividade | SARKIS, J.E.:1080:710:N | pt_BR |
| sigepi.autor.atividade | HORTELLANI, M.A.:713:710:N | pt_BR |