State of the art and current advances on protein cross-linking by irradiation: protein based nanocarriers and bioactive nanoparticles

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dc.contributor.authorVARCA, G.H.C.
dc.contributor.authorMOHAMED, L.B.
dc.contributor.authorFAZOLIN, G.N.
dc.contributor.authorBATISTA, J.G.D.S.
dc.contributor.authorQUEIROZ, R.G.
dc.contributor.authorLUGAO, A.B.
dc.contributor.authorNAVARRO MARQUES, F.A.
dc.contributor.authorFERREIRA, A.H.
dc.coverageInternacionalpt_BR
dc.creator.eventoINTERNATIONAL CONFERENCE ON APPLICATIONS OF RADIATION SCIENCE AND TECHNOLOGYpt_BR
dc.date.accessioned2017-09-21T18:51:13Z
dc.date.available2017-09-21T18:51:13Z
dc.date.eventoApril 24-28, 2017pt_BR
dc.description.abstractThe highlighted role of protein and peptide based delivery systems relies upon the possibility to develop biocompatible drug carriers featuring site specific delivery, biological affinity among unique advantages. Recently, a technique for protein nanostructuring by the use of radiation has been recently reported by our group. Advantages of the use of radiation over conventional methods are related to the possibility to achieve protein cross-linking and sterilization in a single step, as well as the capacity to allow the design of nanocarriers without the need of monomers or toxic cross-linkers. This work reports the use of high energy irradiation towards the design of size-controlled protein-based nanocarriers and bioactive nanoparticles, using bovine serum albumin (BSA) and papain as model protein and protease, respectively, including the state of the art and current advances of the technology. The technique implies on protein desolvation/solvation techniques followed by cross-linking by EB radiation or -irradiation alone, although nanoparticles were also achieved in absence of the cosolvents. Size-controlled BSA nanocarriers were manufactured up to 80 nm and papain bioactive nanoparticles up to 12 nm, as determined by dynamic light scattering. Nanocarrier morphology was evaluated by and negative staining transmission electron microscopy. Protein cross-linking and changes in aromatic the amino acids were evaluated by fluorescence measurements. Biocompatibility experimentswere also performed by means of cytotoxicity and cytokines production. The potential of the systems for the delivery of radiopharmaceuticals or chemotherapeutic agents were also assayed, using technetium or Paclitaxel respectively. In conclusion, the technique allowed the production of biocompatible and bioactive protein nanoparticles suitable for the administration of radiopharmaceuticals and chemotherapeutic agents.pt_BR
dc.event.siglaICARSTpt_BR
dc.format.extent171-171pt_BR
dc.identifier.citationVARCA, G.H.C.; MOHAMED, L.B.; FAZOLIN, G.N.; BATISTA, J.G.D.S.; QUEIROZ, R.G.; LUGAO, A.B.; NAVARRO MARQUES, F.A.; FERREIRA, A.H. State of the art and current advances on protein cross-linking by irradiation: protein based nanocarriers and bioactive nanoparticles. In: INTERNATIONAL CONFERENCE ON APPLICATIONS OF RADIATION SCIENCE AND TECHNOLOGY, April 24-28, 2017, Vienna, Austria. <b>Abstract...</b> Vienna: IAEA, 2017. p. 171-171. Disponível em: http://repositorio.ipen.br/handle/123456789/27769.
dc.identifier.orcidhttps://orcid.org/0000-0002-1737-3191
dc.identifier.urihttp://repositorio.ipen.br/handle/123456789/27769
dc.localViennapt_BR
dc.local.eventoVienna, Austriapt_BR
dc.publisherIAEApt_BR
dc.rightsopenAccesspt_BR
dc.titleState of the art and current advances on protein cross-linking by irradiation: protein based nanocarriers and bioactive nanoparticlespt_BR
dc.typeResumo de eventos científicospt_BR
dspace.entity.typePublication
ipen.autorLINA BADDIA MOHAMED
ipen.autorJORGE GABRIEL DOS SANTOS BATISTA
ipen.autorARYEL HEITOR FERREIRA
ipen.autorADEMAR BENEVOLO LUGAO
ipen.autorRODRIGO GUIMARÃES QUEIROZ
ipen.autorGABRIELA NEMESIO FAZOLIN
ipen.autorGUSTAVO HENRIQUE COSTAVARCA
ipen.codigoautor13998
ipen.codigoautor10274
ipen.codigoautor12722
ipen.codigoautor339
ipen.codigoautor6931
ipen.codigoautor14241
ipen.codigoautor8661
ipen.contributor.ipenauthorLINA BADDIA MOHAMED
ipen.contributor.ipenauthorJORGE GABRIEL DOS SANTOS BATISTA
ipen.contributor.ipenauthorARYEL HEITOR FERREIRA
ipen.contributor.ipenauthorADEMAR BENEVOLO LUGAO
ipen.contributor.ipenauthorRODRIGO GUIMARÃES QUEIROZ
ipen.contributor.ipenauthorGABRIELA NEMESIO FAZOLIN
ipen.contributor.ipenauthorGUSTAVO HENRIQUE COSTAVARCA
ipen.date.recebimento17-09pt_BR
ipen.event.datapadronizada2017pt_BR
ipen.identifier.ipendoc23057pt_BR
ipen.notas.internasAbstractpt_BR
ipen.type.genreResumo
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sigepi.autor.atividadeVARCA, G.H.C.:8661:750:Spt_BR
sigepi.autor.atividadeMOHAMED, L.B.:13998:750:Npt_BR
sigepi.autor.atividadeFAZOLIN, G.N.:14241:740:Npt_BR
sigepi.autor.atividadeBATISTA, J.G.D.S.:10274:740:Npt_BR
sigepi.autor.atividadeQUEIROZ, R.G.:6931:740:Npt_BR
sigepi.autor.atividadeLUGAO, A.B.:339:740:Npt_BR
sigepi.autor.atividadeFERREIRA, A.H.:12722:740:Npt_BR
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