Effects of ionizing radiation on proteins in lyophilized or frozen demineralized

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dc.contributor.authorANTEBI, URI
dc.contributor.authorMATHOR, MONICA B.
dc.contributor.authorSILVA, ANDRE F. da
dc.contributor.authorGUIMARAES, RODRIGO P.
dc.contributor.authorHONDA, EMERSON K.
dc.coverageNacionalpt_BR
dc.date.accessioned2016-11-09T17:33:55Z
dc.date.available2016-11-09T17:33:55Z
dc.date.issued2016pt_BR
dc.description.abstractThe aim was to study the effects of application of ionizing radiation (gamma andelectrons) as sterilizing agents at doses of 15 kGy, 25 kGy and 50 kGy, on lyophilized or frozendemineralized bone tissue for use in transplants.Methods: Five human femoral diaphyses from different donors of musculoskeletal tissuewere demineralized and preserved as lyophilized or frozen at −80◦C. The samples weredivided into two groups: non-irradiated (control) and irradiated by means of gamma rays oran electron beam. The bone proteins were extracted and used to determine the concentra-tions of total protein and BMP 2 and 7.Results: Decreases in total protein and BMP 2 and 7 concentrations were observed. Thedecreases in total protein concentrations, in comparison with the respective control groups,were significant in the lyophilized and frozen samples that were irradiated at a dose of50 kGy of gamma radiation and electron beam, with reductions of more than 30%. Signifi-cant decreases in the levels of BMP 2 and 7 were also observed at higher doses and especiallythrough use of the electron beam.Conclusion: The reductions in the concentrations of total proteins and osteoinductive pro-teins (BMP 2 and 7) were related to the radiation dose, i.e. they increased with higher dosesof ionizing radiation type and the type of bone preservation. The largest reductions in con-centrations were observed in the bones irradiated by means of an electron beam and at adose of 50 kGy. However, this type of radiation and this high dose are not usual practices forsterilization of bone tissuept_BR
dc.format.extent224-230pt_BR
dc.identifier.citationANTEBI, URI; MATHOR, MONICA B.; SILVA, ANDRE F. da; GUIMARAES, RODRIGO P.; HONDA, EMERSON K. Effects of ionizing radiation on proteins in lyophilized or frozen demineralized. <b>Revista Brasileira de Ortopedia</b>, v. 51, n. 2, p. 224-230, 2016. DOI: <a href="https://dx.doi.org/10.1016/j.rboe.2016.02.006">10.1016/j.rboe.2016.02.006</a>. Disponível em: http://repositorio.ipen.br/handle/123456789/26772.
dc.identifier.doi10.1016/j.rboe.2016.02.006pt_BR
dc.identifier.fasciculo2pt_BR
dc.identifier.issn0102-3616pt_BR
dc.identifier.orcidhttp://orcid.org/0000-0002-7294-9106
dc.identifier.urihttp://repositorio.ipen.br/handle/123456789/26772
dc.identifier.vol51pt_BR
dc.relation.ispartofRevista Brasileira de Ortopediapt_BR
dc.rightsopenAccesspt_BR
dc.subjectskeleton
dc.subjectionizing radiations
dc.subjectanimal tissues
dc.subjectproteins
dc.subjectradiation effects
dc.subjectdemineralization
dc.subjectgamma radiation
dc.subjectelectrons
dc.subjectsterilization
dc.subjecttransplants
dc.titleEffects of ionizing radiation on proteins in lyophilized or frozen demineralizedpt_BR
dc.title.alternativeEfeitos da radiação ionizante nas proteinas presentes em ossos humanos desmineralizados liofilizados ou congeladospt_BR
dc.typeArtigo de periódicopt_BR
dspace.entity.typePublication
ipen.autorURI ANTEBI
ipen.autorMONICA BEATRIZ MATHOR
ipen.codigoautor11436
ipen.codigoautor209
ipen.contributor.ipenauthorURI ANTEBI
ipen.contributor.ipenauthorMONICA BEATRIZ MATHOR
ipen.date.recebimento16-11pt_BR
ipen.identifier.fiSem F.I.pt_BR
ipen.identifier.ipendoc22640pt_BR
ipen.type.genreArtigo
relation.isAuthorOfPublicationa8710d24-5089-4a38-9279-ed6e68ef202f
relation.isAuthorOfPublication742b424f-9dfb-4e4a-993b-000052bb1313
relation.isAuthorOfPublication.latestForDiscoverya8710d24-5089-4a38-9279-ed6e68ef202f
sigepi.autor.atividadeANTEBI, URI:11436:230:S
sigepi.autor.atividadeMATHOR, MONICA B.:209:220:N
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