Reconstitution of Leishmania plasma membrane to understand the photodynamic effect

Página do item simplificado
dc.contributor.authorSOUZA, MARESSA D.F. dept_BR
dc.contributor.authorITRI, ROSANGELApt_BR
dc.contributor.authorRIBEIRO, MARTHA S.pt_BR
dc.coverageInternacionalpt_BR
dc.creator.eventoCONGRESS OF THE INTERNATIONAL UNION FOR PURE APPLIED BIOPHYSICS, 20th; ANNUAL MEETING OF THE BRAZILIAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY, 50th; CONGRESS OF BRAZILIAN BIOPHYSICS SOCIETY, 45th; BRAZILIAN SOCIETY ON NUCLEAR BIOSCIENCES CONGRESS, 13thpt_BR
dc.date.accessioned2022-03-25T19:27:31Z
dc.date.available2022-03-25T19:27:31Z
dc.date.eventoOctober 4-8, 2021pt_BR
dc.description.abstractLeishmaniasis is an important neglected disease. Photodynamic therapy (PDT) has been used to fight cutaneous leishmaniasis showing good results. However, PDT mechanisms in Leishmania parasites are not yet completely clarified. In this work, our objective was to develop a protocol to produce giant plasma membrane vesicles (GPMVs) from Leishmania amazonensis promastigotes to understand the mechanisms of action of methylene blue (MB)- mediated PDT on the cell membrane of parasites. For membrane extraction, several techniques were tested. The osmotic shock was the technique that presented the best yield and effectiveness. Phosphate and protein measurements were performed to confirm membrane extraction. For the growth of GPMVs, the best technique was electroforming using different frequencies and voltages in 4 cycles. Reconstituted GPMVs were observed by phasecontrast light microscopy. Subsequently, PDT was applied to GPMVs dispersed in an aqueous solution containing 50 μM MB and we verified the changes in permeability before and after exposure to light. The same process was applied to giant unilamellar vesicles (GUVs) with lipid compositions similar to the parasite membrane. The electroforming technique with the protocol developed in this work made it possible to obtain GPMVs from a promastigote membrane isolate of L. amazonensis. The membrane isolation technique was effective to extract the parasite's membrane while preserving lipids and proteins. In GUVs we observe an increase in the area during PDT in different compositions and loss of contrast. The GPMVs showed a loss of contrast as well as the GUVs but did not show an increase in area. This factor could be explained by the high degree of complexity of the membrane, which contains membrane proteins in addition to containing lipids.pt_BR
dc.event.siglaIUPAB; SBBq; SBBf; SBBNpt_BR
dc.format.extent255-255pt_BR
dc.identifier.citationSOUZA, MARESSA D.F. de; ITRI, ROSANGELA; RIBEIRO, MARTHA S. Reconstitution of Leishmania plasma membrane to understand the photodynamic effect. In: CONGRESS OF THE INTERNATIONAL UNION FOR PURE APPLIED BIOPHYSICS, 20th; ANNUAL MEETING OF THE BRAZILIAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY, 50th; CONGRESS OF BRAZILIAN BIOPHYSICS SOCIETY, 45th; BRAZILIAN SOCIETY ON NUCLEAR BIOSCIENCES CONGRESS, 13th, October 4-8, 2021, São Paulo, SP. <b>Abstract...</b> São Paulo, SP: Sociedade Brasileira de Bioquímica e Biologia Molecular (SBBq), 2021. p. 255-255. Disponível em: http://repositorio.ipen.br/handle/123456789/32871.
dc.identifier.orcid0000-0002-4203-1134pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-4203-1134
dc.identifier.urihttp://repositorio.ipen.br/handle/123456789/32871
dc.localSão Paulo, SPpt_BR
dc.local.eventoSão Paulo, SPpt_BR
dc.publisherSociedade Brasileira de Bioquímica e Biologia Molecular (SBBq)pt_BR
dc.rightsopenAccesspt_BR
dc.subjecttherapy
dc.subjectphotodynamic therapy
dc.subjectprotozoa
dc.subjectparasitic diseases
dc.subjectcell membranes
dc.titleReconstitution of Leishmania plasma membrane to understand the photodynamic effectpt_BR
dc.typeResumo de eventos científicospt_BR
dspace.entity.typePublication
ipen.autorMARTHA SIMOES RIBEIRO
ipen.autorMARESSA DONATO FERREIRA DE SOUZA
ipen.codigoautor574
ipen.codigoautor14904
ipen.contributor.ipenauthorMARTHA SIMOES RIBEIRO
ipen.contributor.ipenauthorMARESSA DONATO FERREIRA DE SOUZA
ipen.date.recebimento22-03
ipen.event.datapadronizada2021pt_BR
ipen.identifier.ipendoc28593pt_BR
ipen.notas.internasAbstractpt_BR
ipen.type.genreResumo
relation.isAuthorOfPublication36215a53-0150-4910-91d7-9559717b62d7
relation.isAuthorOfPublicationd84e8bb6-b3c6-412c-ba73-1d568a16c617
relation.isAuthorOfPublication.latestForDiscoveryd84e8bb6-b3c6-412c-ba73-1d568a16c617
sigepi.autor.atividadeRIBEIRO, MARTHA S.:574:920:Npt_BR
sigepi.autor.atividadeSOUZA, MARESSA D.F. de:14904:920:Spt_BR
Pacote Original
Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
28593.pdf
Tamanho:
723.83 KB
Formato:
Adobe Portable Document Format
Descrição:
Baixar
Licença do Pacote
Agora exibindo 1 - 1 de 1
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Baixar
Coleções
Eventos - Resumos