Photodynamic activity on biofilm in endotracheal tubes of patients admitted to an intensive care unit

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dc.contributor.authorSOARES, ROSANE B.pt_BR
dc.contributor.authorCOSTA, DENIS H.pt_BR
dc.contributor.authorMIYAKAWA, WALTERpt_BR
dc.contributor.authorDELGADO, MARIA G.T.pt_BR
dc.contributor.authorGARCEZ, AGUINALDO S.pt_BR
dc.contributor.authorYOSHIMURA, TANIA M.pt_BR
dc.contributor.authorRIBEIRO, MARTHA S.pt_BR
dc.contributor.authorNUNEZ, SILVIA C.pt_BR
dc.coverageInternacionalpt_BR
dc.date.accessioned2020-10-08T15:07:10Z
dc.date.available2020-10-08T15:07:10Z
dc.date.issued2020pt_BR
dc.description.abstractVentilator-associated pneumonia (VAP) is an infection that arises after endotracheal intubation affecting patients under intensive care. The presence of the endotracheal tube (ETT) is a risk factor since it is colonized by multispecies biofilm. Antimicrobial photodynamic therapy (aPDT) could be a strategy to decontaminate ETTs. We verify if methylene blue (MB) associated with external illumination of the ETT could be an alternative to destroy biofilm. We performed an in vitro and ex vivo study. In vitro study was performed with P. aeruginosa biofilm grew over ETT for 7 days. After treatment, the surviving cells were cultured for 3 days and the biofilm was analyzed by crystal violet absorbance. Ex vivo study employed ETT obtained from extubated patients. aPDT was performed with MB (100 μM) and red LED (k = 640±20 nm). We quantified the biofilm thickness and used scanning electron microscopy and fluorescence technique to verify morphological and functional changes after aPDT. Our results showed that bacteria remain susceptible to aPDT after sequential treatments. We also attested that aPDT can reduce biofilm thickness, disrupt biofilm attachment from ETT surface and kill microbial cells. These data suggest that aPDT should be investigated to decrease VAP incidence via ETT decontamination.pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorshipIDCNPq: 465763/2014-6pt_BR
dc.format.extent618-624pt_BR
dc.identifier.citationSOARES, ROSANE B.; COSTA, DENIS H.; MIYAKAWA, WALTER; DELGADO, MARIA G.T.; GARCEZ, AGUINALDO S.; YOSHIMURA, TANIA M.; RIBEIRO, MARTHA S.; NUNEZ, SILVIA C. Photodynamic activity on biofilm in endotracheal tubes of patients admitted to an intensive care unit. <b>Photochemistry and Photobiology</b>, v. 96, n. 3, p. 618-624, 2020. SI. DOI: <a href="https://dx.doi.org/10.1111/php.13239">10.1111/php.13239</a>. Disponível em: http://repositorio.ipen.br/handle/123456789/31414.
dc.identifier.doi10.1111/php.13239pt_BR
dc.identifier.fasciculo3pt_BR
dc.identifier.issn0031-8655pt_BR
dc.identifier.orcid0000-0002-4203-1134pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-4203-1134
dc.identifier.percentilfi48.47pt_BR
dc.identifier.percentilfiCiteScore73.00
dc.identifier.suplementoSIpt_BR
dc.identifier.urihttp://repositorio.ipen.br/handle/123456789/31414
dc.identifier.vol96pt_BR
dc.relation.ispartofPhotochemistry and Photobiologypt_BR
dc.rightsopenAccesspt_BR
dc.subjectrespiratory system
dc.subjectintratracheal administration
dc.subjectpneumonia
dc.subjectrespiratory system diseases
dc.subjectbacteria
dc.subjectbiological materials
dc.subjectmicroorganisms
dc.subjectphotochemistry
dc.subjectpatients
dc.subjectmedicine
dc.subjecthospitals
dc.titlePhotodynamic activity on biofilm in endotracheal tubes of patients admitted to an intensive care unitpt_BR
dc.typeArtigo de periódicopt_BR
dspace.entity.typePublication
ipen.autorMARTHA SIMOES RIBEIRO
ipen.autorTANIA MATEUS YOSHIMURA
ipen.codigoautor574
ipen.codigoautor10230
ipen.contributor.ipenauthorMARTHA SIMOES RIBEIRO
ipen.contributor.ipenauthorTANIA MATEUS YOSHIMURA
ipen.date.recebimento20-10
ipen.identifier.fi3.421pt_BR
ipen.identifier.fiCiteScore5.3
ipen.identifier.ipendoc27188pt_BR
ipen.identifier.iwosWoSpt_BR
ipen.identifier.ods3
ipen.range.fi3.000 - 4.499
ipen.range.percentilfi25.00 - 49.99
ipen.type.genreArtigo
relation.isAuthorOfPublication36215a53-0150-4910-91d7-9559717b62d7
relation.isAuthorOfPublication36384aec-6adf-42ef-99ae-68f62b9a69b9
relation.isAuthorOfPublication.latestForDiscovery36384aec-6adf-42ef-99ae-68f62b9a69b9
sigepi.autor.atividadeRIBEIRO, MARTHA S.:574:920:Npt_BR
sigepi.autor.atividadeYOSHIMURA, TANIA M.:10230:920:Npt_BR
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