The biochemical mechanisms of antimicrobial photodynamic therapy

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dc.contributor.authorSABINO, CAETANO P.pt_BR
dc.contributor.authorRIBEIRO, MARTHA S.pt_BR
dc.contributor.authorWAINWRIGHT, MARKpt_BR
dc.contributor.authorANJOS, CAROLINA dospt_BR
dc.contributor.authorSELLERA, FABIO P.pt_BR
dc.contributor.authorDROPA, MILENApt_BR
dc.contributor.authorNUNES, NATHALIA B.pt_BR
dc.contributor.authorBRANCINI, GUILHERME T.P.pt_BR
dc.contributor.authorBRAGA, GILBERTO U.L.pt_BR
dc.contributor.authorARANA-CHAVEZ, VICTOR E.pt_BR
dc.contributor.authorFREITAS, RAUL O.pt_BR
dc.contributor.authorLINCOPAN, NILTONpt_BR
dc.contributor.authorBAPTISTA, MAURICIO S.pt_BR
dc.coverageInternacionalpt_BR
dc.date.accessioned2023-04-26T18:51:30Z
dc.date.available2023-04-26T18:51:30Z
dc.date.issued2023pt_BR
dc.description.abstractThe unbridled dissemination of multidrug-resistant pathogens is a major threat to global health and urgently demands novel therapeutic alternatives. Antimicrobial photodynamic therapy (aPDT) has been developed as a promising approach to treat localized infections regardless of drug resistance profile or taxonomy. Even though this technique has been known for more than a century, discussions and speculations regarding the biochemical mechanisms of microbial inactivation have never reached a consensus on what is the primary cause of cell death. Since photochemically generated oxidants promote ubiquitous reactions with various biomolecules, researchers simply assumed that all cellular structures are equally damaged. In this study, biochemical, molecular, biological and advanced microscopy techniques were employed to investigate whether protein, membrane or DNA damage correlates better with dose-dependent microbial inactivation kinetics. We showed that although mild membrane permeabilization and late DNA damage occur, no correlation with inactivation kinetics was found. On the other hand, protein degradation was analyzed by three different methods and showed a dose-dependent trend that matches microbial inactivation kinetics. Our results provide a deeper mechanistic understanding of aPDT that can guide the scientific community toward the development of optimized photosensitizing drugs and also rationally propose synergistic combinations with antimicrobial chemotherapy.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorshipIDFAPESP: 19/10851-4; 16/25095-2; 13/07937-8; 17/22406-0; 16/08593-9pt_BR
dc.description.sponsorshipIDCNPq: 141901/2016-0; 312249/2017-9; 465763/2014-6; 433128/2018-6pt_BR
dc.format.extent742-750pt_BR
dc.identifier.citationSABINO, CAETANO P.; RIBEIRO, MARTHA S.; WAINWRIGHT, MARK; ANJOS, CAROLINA dos; SELLERA, FABIO P.; DROPA, MILENA; NUNES, NATHALIA B.; BRANCINI, GUILHERME T.P.; BRAGA, GILBERTO U.L.; ARANA-CHAVEZ, VICTOR E.; FREITAS, RAUL O.; LINCOPAN, NILTON; BAPTISTA, MAURICIO S. The biochemical mechanisms of antimicrobial photodynamic therapy. <b>Photochemistry and Photobiology</b>, v. 99, n. 2, p. 742-750, 2023. DOI: <a href="https://dx.doi.org/10.1111/php.13685">10.1111/php.13685</a>. Disponível em: http://repositorio.ipen.br/handle/123456789/34012.
dc.identifier.doi10.1111/php.13685pt_BR
dc.identifier.fasciculo2pt_BR
dc.identifier.issn0031-8655pt_BR
dc.identifier.orcid0000-0002-4203-1134pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-4203-1134
dc.identifier.percentilfi51.7pt_BR
dc.identifier.percentilfiCiteScore64.00pt_BR
dc.identifier.urihttp://repositorio.ipen.br/handle/123456789/34012
dc.identifier.vol99pt_BR
dc.relation.ispartofPhotochemistry and Photobiologypt_BR
dc.rightsopenAccesspt_BR
dc.subjectantimicrobial agents
dc.subjectphotodynamic therapy
dc.subjectbiochemical reaction kinetics
dc.subjectmicrobial drug resistance
dc.titleThe biochemical mechanisms of antimicrobial photodynamic therapypt_BR
dc.typeArtigo de periódicopt_BR
dspace.entity.typePublication
ipen.autorMARTHA SIMOES RIBEIRO
ipen.codigoautor574
ipen.contributor.ipenauthorMARTHA SIMOES RIBEIRO
ipen.date.recebimento23-04
ipen.identifier.fi3.3pt_BR
ipen.identifier.fiCiteScore6.1pt_BR
ipen.identifier.ipendoc29636pt_BR
ipen.identifier.iwosWoSpt_BR
ipen.range.fi3.000 - 4.499
ipen.range.percentilfi50.00 - 74.99
ipen.type.genreArtigo
relation.isAuthorOfPublication36215a53-0150-4910-91d7-9559717b62d7
relation.isAuthorOfPublication.latestForDiscovery36215a53-0150-4910-91d7-9559717b62d7
sigepi.autor.atividadeRIBEIRO, MARTHA S.:574:920:Npt_BR
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