In vivo genotoxicity of 2-Alkylcyclobutanones in liver cells from rats fed with irradiated cocoa butter using flow citometry

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dc.contributor.authorMARTINS, REGIANEpt_BR
dc.contributor.authorVIEIRA, DANIEL P.pt_BR
dc.contributor.authorCARVALHO, LUMA R. dept_BR
dc.contributor.authorBARBEZAN, ANGELICA B.pt_BR
dc.contributor.authorVILLAVICENCIO, ANNA L.C.H.pt_BR
dc.coverageInternacionalpt_BR
dc.creator.eventoINTERNATIONAL NUCLEAR ATLANTIC CONFERENCEpt_BR
dc.date.accessioned2020-01-09T00:19:40Z
dc.date.available2020-01-09T00:19:40Z
dc.date.eventoOctober 21-25, 2019pt_BR
dc.description.abstractFood irradiation proves to be an effective technique of eliminating some pathogens from food and this has gained significant attention to its potential for food safety. Since 1990, studies on the toxicological safety of 2-Alkylcyclobutanones have been conducted extensively. 2- Alkylcyclobutanones are unique radiolytic products generated by the radiation-induced breakage of triglycerides in food, are exclusively found in irradiated lipid containing foods. 2-dodecylcyclobutanone (2-dDCB) and 2-tetradecylcyclobutanone (2-tDCB) are the predominant compounds detected in irradiated food. Despite studies showing non- genotoxicity of 2-ACBs (2-Alkylcyclobutanones), the results are conflicting and therefore we continue the studies in order to confirm the compounds safety for human health. In vivo micronucleus test were performed to verify the 2-ACBs genotoxic effects in hepatic cells using flow citometry. We used cocoa butter irradiated with 20 kGy at IPEN GAMACELL. A group with animals (IPEN Ethical Animal Experimentation Committee, process number 148/14) was treated with daily intake of irradiated cocoa butter, synthesized 2- Dodecylcyclobutanone and 2-Tetradecylcyclobutanone for two months. Hepatic cells were selected for genotoxicity analysis due to the liver importance in the compounds metabolization. Analyzes were made by micronucleus test with specific cells extracted from hepatic tissue using flow cytometry, which is an alternative to conventional techniques, allowing faster analysis and reduction in the animals number that is a subject much approached in research today. The improvement of the analytical techniques is important for the research future since the irradiation process is already consolidated. The results confirmed the safety of the food irradiation process, as they did not indicate the genotoxic potential of the samples.pt_BR
dc.event.siglaINACpt_BR
dc.format.extent2070-2078pt_BR
dc.identifier.citationMARTINS, REGIANE; VIEIRA, DANIEL P.; CARVALHO, LUMA R. de; BARBEZAN, ANGELICA B.; VILLAVICENCIO, ANNA L.C.H. In vivo genotoxicity of 2-Alkylcyclobutanones in liver cells from rats fed with irradiated cocoa butter using flow citometry. In: INTERNATIONAL NUCLEAR ATLANTIC CONFERENCE, October 21-25, 2019, Santos, SP. <b>Proceedings...</b> Rio de Janeiro: Associação Brasileira de Energia Nuclear, 2019. p. 2070-2078. Disponível em: http://repositorio.ipen.br/handle/123456789/30603.
dc.identifier.orcid0000-0001-6199-7877pt_BR
dc.identifier.orcid0000-0002-0007-534Xpt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-6199-7877
dc.identifier.orcidhttps://orcid.org/0000-0002-0007-534X
dc.identifier.urihttp://repositorio.ipen.br/handle/123456789/30603
dc.localRio de Janeiropt_BR
dc.local.eventoSantos, SPpt_BR
dc.publisherAssociação Brasileira de Energia Nuclear
dc.rightsopenAccesspt_BR
dc.subjectalkyl radicals
dc.subjectbutter
dc.subjectcell flow systems
dc.subjectcocoa products
dc.subjectfood processing
dc.subjectgenetic effects
dc.subjectliver cells
dc.subjectmice
dc.subjectradiation doses
dc.subjecttoxicity
dc.titleIn vivo genotoxicity of 2-Alkylcyclobutanones in liver cells from rats fed with irradiated cocoa butter using flow citometrypt_BR
dc.typeTexto completo de eventopt_BR
dspace.entity.typePublication
ipen.autorREGIANE MARTINS
ipen.autorLUMA RAMIREZ DE CARVALHO
ipen.autorANNA LUCIA CASANAS HAASIS VILLAVICENCIO
ipen.autorANGELICA BUENO BARBEZAN
ipen.autorDANIEL PEREZ VIEIRA
ipen.codigoautor14027
ipen.codigoautor12133
ipen.codigoautor1372
ipen.codigoautor6417
ipen.codigoautor3158
ipen.contributor.ipenauthorREGIANE MARTINS
ipen.contributor.ipenauthorLUMA RAMIREZ DE CARVALHO
ipen.contributor.ipenauthorANNA LUCIA CASANAS HAASIS VILLAVICENCIO
ipen.contributor.ipenauthorANGELICA BUENO BARBEZAN
ipen.contributor.ipenauthorDANIEL PEREZ VIEIRA
ipen.date.recebimento20-01
ipen.event.datapadronizada2019pt_BR
ipen.identifier.ipendoc26253pt_BR
ipen.notas.internasProceedingspt_BR
ipen.type.genreArtigo
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relation.isAuthorOfPublication8ce58b2f-23cf-45b5-a8c4-0fb067965096
relation.isAuthorOfPublication27082032-b0c8-462c-8d6c-767873119adf
relation.isAuthorOfPublicationc2352608-be9c-4a73-be8c-571f10bb53d2
relation.isAuthorOfPublication.latestForDiscoveryad3692a6-2f6b-4b47-9284-c6eee5d2084d
sigepi.autor.atividadeVILLAVICENCIO, ANNA L.C.H.:1372:210:Npt_BR
sigepi.autor.atividadeBARBEZAN, ANGELICA B.:6417:110:Npt_BR
sigepi.autor.atividadeCARVALHO, LUMA R. de:12133:810:Npt_BR
sigepi.autor.atividadeVIEIRA, DANIEL P.:3158:810:Npt_BR
sigepi.autor.atividadeMARTINS, REGIANE:14027:210:Spt_BR
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