Microwave-mediated synthesis of iron-oxide nanoparticles for use in magnetic levitation cell cultures
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2019
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Applied Nanoscience
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The use of three-dimensional cell cultures has been widely used for efficacy and/or toxicity testing of compounds. One of the
most promising systems, based on magnetic levitation, is dependent on proper cell magnetization, achieved through adsorption
of iron-oxide nanoparticles on cell membranes. These particles must bare not only significant responses to magnetic
fields, but also a stable mechanism to attachment to cells. This work proposes a simple, one-pot synthesis method to produce
magnetite nanoparticles, using a Fe2+
precursor associated with amino acids under microwave heating, and successive
steps to confer positive charges to particles. X-ray diffraction could confirm Fe3O4
composition, and TEM analysis showed
cubic-like crystallites with less than 50 nm. Zeta-potential experiments showed that particles remained positively charged
(20.98 ± 0.28 mV) in physiological pH, suggesting ability to attach to (negatively charged) cell membranes, observed through
optical microscopy. Iron colloid was found to be non-cytotoxic in concentrations up to 8% in cell culture media. Finally,
human prostate cancer cells were cultured in 96-well plates using magnetic levitation and could be kept 8 days in culture.
The results showed a feasible way to produce spheroids relying on magnetic levitation, using a newly described method of
magnetic and cell adherent nanoparticle production.
Como referenciar
BONFIM, LETICIA; PASSOS, PRISCILA de Q.S.; GONÇALVES, KARINA de O.; COURROL, LILIA C.; SILVA, FLAVIA R. de O.; VIEIRA, DANIEL P. Microwave-mediated synthesis of iron-oxide nanoparticles for use in magnetic levitation cell cultures. Applied Nanoscience, v. 9, n. 8, p. 1707-1717, 2019. DOI: 10.1007/s13204-019-00962-1. Disponível em: http://repositorio.ipen.br/handle/123456789/30817. Acesso em: 27 Apr 2024.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.