Growth hormone receptor deletion reduces the density of axonal projections from hypothalamic arcuate nucleus neurons
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2020
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The arcuate nucleus (ARH) is an important hypothalamic area for the homeostatic control of feeding
and other metabolic functions. In the ARH, proopiomelanocortin- (POMC) and agouti-related peptide (AgRP)-
expressing neurons play a key role in the central regulation of metabolism. These neurons are influenced by circulating
factors, such as leptin and growth hormone (GH). The objective of the present study was to determine
whether a direct action of GH on ARH neurons regulates the density of POMC and AgRP axonal projections to
major postsynaptic targets. We studied POMC and AgRP axonal projections to the hypothalamic paraventricular
(PVH), lateral (LHA) and dorsomedial (DMH) nuclei in leptin receptor (LepR)-deficient mice (Leprdb/db),
GH-deficient mice (Ghrhrlit/lit) and in mice carrying specific ablations of GH receptor (GHR) either in LepR- or
AgRP-expressing cells. Leprdb/db mice presented reduction in the density of POMC innervation to the PVH compared
to wild-type and Ghrhrlit/lit mice. Additionally, both Leprdb/db and Ghrhrlit/lit mice showed reduced AgRP fiber
density in the PVH, LHA and DMH. LepR GHR knockout mice showed decreased density of POMC innervation in
the PVH and DMH, compared to control mice, whereas a reduction in the density of AgRP innervation was
observed in all areas analyzed. Conversely, AgRP-specific ablation of GHR led to a significant reduction in AgRP
projections to the PVH, LHA and DMH, without affecting POMC innervation. Our findings indicate that GH has
direct trophic effects on the formation of POMC and AgRP axonal projections and provide additional evidence that
GH regulates hypothalamic neurocircuits controlling energy homeostasis.
Como referenciar
WASINSKI, FREDERICK; FURIGO, ISADORA C.; TEIXEIRA, PRYSCILA D.S.; RAMOS-LOBO, ANGELA M.; PERONI, CIBELE N.; BARTOLINI, PAOLO; LIST, EDWARD O.; KOPCHICK, JOHN J.; DONATO JUNIOR, JOSE. Growth hormone receptor deletion reduces the density of axonal projections from hypothalamic arcuate nucleus neurons. Neuroscience, v. 434, p. 136-147, 2020. DOI: 10.1016/j.neuroscience.2020.03.037. Disponível em: http://repositorio.ipen.br/handle/123456789/31408. Acesso em: 08 May 2024.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.