AMANDA CARAMEL JUVINO

AMANDA CARAMEL JUVINO

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Caries preventive action of Nd:YAG and fluoride in three different pH conditions
2023 - JUVINO, AMANDA C.; ZANINI, NATHALIA A.; AVELINO, SABRINA G.; OLIVEIRA, YASMIN R.F. de; GERMANO, GLEICE C.M.; EDUARDO, CARLOS de P.; ZEZELL, DENISE M.
This in vitro study aimed to evaluate the preventive action of topical fluoride application combined with laser irradiation under different pH conditions using Fourier Transform Infrared Spectroscopy (FTIR) and Scanning ElectronMicroscopy (SEM). A total of 180 samples of human dental enamel were prepared and divided into groups: Negative Control, Fluoride (FFA 12.300 F􀀀/g), Laser (Nd:YAG 84.9 J/cm2), and Laser + Fluoride (Nd:YAG 84.9 J/cm2 + FFA 12.300 F􀀀/g). The pH cycling was performed at three different pH conditions: pH 5 (below the critical pH for hydroxyapatite), pH 4.5 (below the critical pH in the presence of fluorapatite), and pH 4 (investigating acid resistance of hydroxyapatite and fluorapatite forms with laser irradiation). In the FTIR analysis, the Laser + Fluoride group demonstrated statistically significant differences compared to the Negative Control group and Fluoride group at pH 4.5 and pH 4 when evaluating the phosphate bands. Similar results were observed in the SEM analysis, where the Laser + Fluoride group exhibited lower demineralization compared to the other treatments at pH 4.5 and pH 4. In conclusion, the Laser + Fluoride group demonstrated a significant reduction in demineralization even at pH levels below the critical threshold for fluorapatite, highlighting its superior acid resistance compared to fluoride alone.
Exploring enamel demineralization from SEM images using deep learning algorithms
2022 - FAROOQ, SAJID; CARAMEL-JUVINO, AMANDA; FONTES, YASMIN R.; GARDIANO, SABRINA A.; ZEZELL, DENISE M.
Here, we employ segmentation and convolutional neural network (CNN) to identify and quantify enamel demineralization. Our results depict that CNN model using input SEM images achieve accuracy up to 79% for enamel demineralization diagnosis.
Superior Machine Learning Method for breast cancer cell lines identification
2022 - FAROOQ, SAJID; CARAMEL-JUVINO, AMANDA; DEL-VALLE, MATHEUS; SANTOS, SOFIA; BERNARDES, EMERSON S.; ZEZELL, DENISE M.
We propose an artificial intelligence platform based on machine learning (ML) algorithm using Neighborhood Component analysis and K-Nearest Neighbors for breast cancer cell lines recognition. Our model presents up to 97% accuracy for identification of breast cancer cell lines.
Identification of enamel demineralization using high performance convolutional neural network
2022 - CARAMEL-JUVINO, AMANDA; FAROOQ, SAJID; ROMANO, MARIANA; ZEZELL, DENISE M.
Here, we traces use segmentation and convolutional neural network (CNN) to trace, diagnose and quantify enamel demineralization for research. The preprocessing, histograms based methods are used to enhance the contrast and equalize the brightness through the scanning electron microscope images. Our result evidence that the deep learning based CNN model is highly efficient to process the dental image to achieve high accuracy of enamel demineralization and presents promising outcomes with optimal precision.
Protocolos de associação de flúor e laser de alta potência (Nd:YAG) na prevenção da cárie
2022 - FONTES-OLIVEIRA, Y.R.; CARAMEL-JUVINO, A.; AVELINO, S.G.; ZEZELL, D.M.
Avaliar os protocolos publicados para prevenção da cárie utilizando associação de flúor e laser Nd:YAG. Foram utilizadas seis bases de dados: Web-of-Science, PubMed, ResearchGate, Google Acadêmico, LILACS e Scielo. Dos 115 artigos inicialmente coletados, foram excluídas as duplicatas, artigos com dentes bovinos, artigos sem flúor gel ou que abordavam erosão. Foram selecionados 12 artigos. Sobre os efeitos do tratamento, os artigos relataram que a irradiação laser promove fusão e re-solidificação da superfície, tornando-a mais ácido-resistente. Quando associado a fluoretos, este laser aumenta a superfície de contato para a ação do flúor, potencializando os seus efeitos. Analisando os parâmetros utilizados na irradiação, a maior parte (50%) usou 0,6W de potência e 84,9 J/cm² de fluência, seguido pelos valores menores 0,5W e 70,7 J/cm² (16,6%). Estes parâmetros foram considerados seguros em estudos que avaliam a possibilidade de dano térmico à polpa. Dentre os estudos que compararam os efeitos da aplicação de flúor prévia ou posteriormente à irradiação laser, apenas 8,3% relataram que o tratamento sinérgico com laser de Nd:YAG e flúor não foi mais eficaz do que o flúor isolado. Já 33,3% apresentaram melhores efeitos com a aplicação de flúor depois do laser e 8,3% obtiveram melhores resultados com aplicação antes do laser. 8,3% concluíram que o resultado independe da ordem. O protocolo de 0,6W de potência e 84,9 J/cm² de fluência, com aplicação de flúor posterior a irradiação laser, apresentou resultados satisfatórios na prevenção da cárie para a maioria dos estudos.
Avaliação da desmineralização superficial do esmalte tratado com flúor e laser utilizando Microscopia Eletrônica de Varredura
2022 - CARAMEL-JUVINO, A.; FONTES-OLIVEIRA, Y.R.; AVELINO, S.G.; ZEZELL, D.M.
Avaliar o efeito da aplicação tópica de flúor (12.300 μF-/g) associada a irradiação com laser de Nd:YAG (84 J/cm2) na desmineralização superficial do esmalte, utilizando imagens de Microscopia Eletrônica de Varredura (MEV). Utilizou-se 60 amostras de esmalte humano (CAAE: 02854118.3.0000.0075), divididas em 4 grupos: Controle Negativo, Flúor, Laser e Flúor + Laser (n=15). Após os tratamentos, os grupos foram subdivididos em 3 (n=5), para ciclagem de pH in vitro simulando três condições diferentes: pH 5 (abaixo do pH crítico do esmalte), pH 4,5 (abaixo do pH crítico do esmalte na presença do flúor) e pH 4 (investigativo). Quatro imagens por amostras foram obtidas por MEV a 15 kV, em aumento de 4.000X. As imagens foram classificadas como esmalte hígido, desmineralização leve, moderada ou severa. Para análise estatística, realizou-se uma normalização, onde a classificação de desmineralização severa foi equiparada a 100% de desmineralização, 0 foi equiparado à classificação saudável e as demais classificações foram proporcionais. Em pH 5, os grupos Flúor, Laser e Flúor + Laser não tiveram diferenças significativas entre si (p>0,05). Em pH 4,5, o grupo Flúor + Laser teve o menor percentual de desmineralização (p<0,05). Já em pH 4, não houve diferença significativa entre nenhum grupo. Os dados sugerem que o tratamento de Flúor + Laser tem ação preventiva mesmo em pH abaixo do crítico para esmalte na presença do flúor (pH 4,5). Além disso, indica que possivelmente este tratamento altera o pH crítico da estrutura para valores entre 4,5 e 4, ampliando o poder preventivo do flúor.
Erbium laser in the debonding of ceramics
2022 - ZANINI, NATHALIA A.; CARAMEL-JUVINO, AMANDA; ANA, PATRICIA A.; ZEZELL, DENISE M.
Background: The debonding of crowns and ceramic veneers with laser is already a reality in the clinic. It presents benefits in comparison to traditional removal with the use of a rotating instrument; however, there is still no consolidated protocol and many professionals use it without the necessary scientific basis. Objective: The aim of this work was to perform a literature review on the debonding of indirect ceramic restorations using the Er:YAG and Er,Cr:YSGG lasers to provide clinical professionals and the scientific community a careful analysis, and also to guiding the use of irradiation at laser for such procedures. Methods: Bibliographic searches were performed in the following databases: Pubmed, Web of Science and Google Scholar. According to the inclusion criteria adopted, twenty-seven clinical and in vitro studies were selected in the period from 2007 to 2021. In the present work, the composition of the crowns and facets, as well as the types of cement and the laser irradiation protocols adopted in the selected studies is detailed. Conclusions: It was concluded that the use of erbium lasers for debonding of indirect ceramic restorations proved to be more selective and conservative when compared to removal with a rotary diamond instrument. Furthermore, it was shown to be in more efficient in debonding different types of ceramics. However, there are great variations in the “debonding” protocols, which emphasizes the need for further studies that seek to standardize the irradiation protocols considering the different clinical situations.
Evaluation of the anti-caries effect beyond the critical enamel pH of preventive treatment of fluoride associated with Nd:YAG laser irradiation
2021 - JUVINO, AMANDA C.; ROMANO, MARIANA; ALVES, NATHALIA Z.; FREITAS, THAIS R.; ZAMATARO, CLAUDIA B.; ZEZELL, DENISE M.
This study aimed to evaluate the anti-caries effect of fluoride associated with Nd:YAG laser irradiation in the h-eatment of enamel Eight groups (n = S) were analyzed: Negative Control pH 4.5; Negative control pH 4; Fluoride pH 4.5; Fluoride pH 4; Nd-YAG pH 4.5; Nd-YAG pH 4; (Fluoride + Nd-YAG) pH 4.5 and (Fluoride + Nd-YAG) pH 4. All samples were analyzed by Scanning Electron Microscopy (SEM) before and after the cycle. Quantification of phosphorus in the cycling solutions was carried out using the colorimetric method, as an indication of enamel demineralization. The anti-caries effect of the Fluoride + Nd: YAG treatment in addition to the critical pH of the enamel can be observed in the morphological analysis, however in the analysis of the demineralization solutions, the amount of phosphorus showed a difference only in the Fluoiide group compared to the others in the investigative pH.
Analysis of ceramic laminates removal with Er,Cr:YSGG laser by optical coherence tomography
2019 - ZANINI, NATHALIA; ZAMATARO, CLAUDIA B.; RABELO, THAIS F.; JUVINO, AMANDA C.; KUCHAR, NIELSEN G.; CASTRO, PEDRO; ANA, PATRICIA da; ZEZELL, DENISE
Porcelain laminated veneers have been widely used. For wear of hard tissue such as enamel and dentin, the diamond rotary instrument is the most traditional, but the laser has become recently used to remove aesthetic facets. Optical coherence tomography (OCT) used as an optical biopsy, is important for morphological analysis and attenuation coefficient is related to the property of the photons to be scattered by the samples. After approval by the Ethics Committee, the present study investigated the detachment of 30 ceramic E-max fragments cemented in human dental enamel of dimensions 3mm x 3mm x 0.7mm with 3 types of resin cements, RelxY Veneer, Relx U200 and Variolink Veneer. The samples (Enamel + Ceramic Fragment) were randomly distributed in the 3 groups and cemented according to the manufacturer. After that, they were prepared for irradiation with the Er,Cr: YSSG laser under predetermined conditions (3.5 and 3W, 20Hz, 60% water and 40% air flow). OCT analysis was done before and after irradiation. We observed that themorphological changes of the enamel surface showed an increased surface area due to the cement remaining in the enamel.We concluded that the Er, Cr: YSGG laser, when used in the irradiation protocol tested, seems to be a safe tool for the removal of laminates.
Removal of laminates with Er,Cr:YSGG laser from dental enamel submitted to gamma radiation
2019 - RABELO, THAIS F.; ZAMATARO, CLAUDIA B.; ZANINI, NATHALIA; JUVINO, AMANDA C.; KUCHAR, NIELSEN G.; CASTRO, PEDRO A.A.; ZEZELL, DENISE M.
Background: Modern Dentistry is characterized by the search for aesthetic perfection in the smile. Many efforts are made regarding procedures related to manufacturing, cementing, maintenance and removal of ceramic laminates. The laser removal of laminates has become more frequent when the aesthetical procedures needs to be replaced. On the other hand, the head and neck cancer treatment causes comorbidities in the buccal environment when laminates are in place. Objective: To evaluate in vitro the removal of ceramic fragments by means of Er,Cr:YSGG laser irradiation, after gamma radiation. Methods: 20 Lithium disilicate veneers were cemented with Variolink to human dental enamel and then samples were irradiated with 0.07 kGy. After gamma irradiation, the laminates removal with Er,Cr:YSGG laser was performed. The control group was not subjected to gamma radiation. Dental enamel samples were analyzed by Scanning Electron Microscopy (SEM), Fourier Transformed Infrared Spectroscopy (FTIR) and Surface Microhardness Loss (SMH). Results: SEM has shown that less cement was found after removal of the facets in gamma irradiated group when compared to the control. For both groups there was alteration of the SMH possibly due to the use of the adhesive system. When performed intra group analysis, the sample being its own control of baseline in the FTIR analysis, there were no band shifts or formation of new compounds on the surface of human dental enamel. Conclusions: The Er,Cr:YSGG laser is an effective alternative for laminates removal in human dental enamel even when it is subjected to gamma radiation.