BIANCA GUIMARAES NEGRAO

BIANCA GUIMARAES NEGRAO

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Physical properties of irradiated Sapodilla (Manilkara zapota)
2021 - NEGRAO, B.G.; KOIKE, A.C.R; LANA, J.G.; VILLAVICENCIO, A.L.C.H.
Effects of electron-beam and gamma irradiation on the antioxidant compounds of edible flowers species
2021 - KOIKE, A.C.R.; NEGRAO, B.G.; ARAUJO, E.S.; ALMEIDA-MURADIAN, L.B.; VILLAVICENCIO, A.L.C.H.
Analysis of carotenoids in edible flowers of Dianthus chinensis processed by ionizing radiation
2021 - KOIKE, A.C.R.; ARAUJO, E.S.; NEGRAO, B.G.; ALMEIDA-MURADIAN, L.B.; VILLAVICENCIO, A.L.C.H.
Dianthus chinensis flowers are widely used in culinary preparations and are appreciated because of its bioactive compounds. It is a perishable food that should be grown without the use of pesticides. In this context, food irradiation is a process that has proven to be an efficient tool in preserving and extending the perishable product shelf life without changing the temperature or leaving residues. The purpose of this study was to evaluate carotenoids in D. chinensis flowers submitted to gamma irradiation and electron beam doses of 0.5, 0.8, and 1.0 kGy. High-performance liquid chromatography for carotenoid determination was used. In the edible flower analyzed was found carotenoid lutein (4.02 to 7.52 mg/ 100 g). The lutein was higher for irradiated samples, especially those treated with 0.8 and 1.0 kGy in both irradiation technology. In conclusion, the lutein amount in the chinese pink enhances as the dose increases, and the applied irradiation treatments represent a feasible technology to preserve the nutritional quality of edible flower petals as well as attend to food safety requirements.
Application of ionizing radiation in the "licuri" (Syagrus coronata (Mart.)) as phytosanitary treatment of the Pachymerus nucleorum beetle (Fabricius, 1792)
2021 - NOGUEIRA, A.G.; NEGRAO, B.G.; ARTHUR, V.; VILLAVICENCIO, A.L.C.H.
Pachymerus nucleorum (Fabricius, 1792), is a pest of economic importance it causes direct damages to the “licuri” almonds. The objective of this study was to determine the lethal doses of gamma radiation for stages egg, larvae, and pupae of this insect as a phytosanitary treatment in “licuri”. The samples were irradiated in a Cobalt 60 irradiator, Gammacell-220 type, installed at the Institute of Energy and Nuclear Research - IPEN / CNEN at a dose rate of 748 Gy / h. Each treatment consisted of 3 replicates, so 180 licuri were irradiated in the doses of 50, 75, 100, and 125 Gy. After irradiation, the samples were stored at a temperature of 25 ± 5°C and relative humidity of 70 ± 5%. The evaluation of the fruits was performed by the emergence of insects. It is concluded that the dose of 125 Gy was sufficient to inhibit the development of insect life stages. Therefore, this dose of radiation can be used for control of the immature stages of the P. nucleorum.
Effects of electron beam irradiation on the bioactive components of goji-berry
2021 - RODRIGUES, FLAVIO T.; KOIKE, AMANDA C.R.; SILVA, PAMELA G. da; NEGRAO, BIANCA G.; ALENCAR, SEVERINO M. de; MANCINI FILHO, JORGE; VILLAVICENCIO, ANNA L.C.H.
The goji-berry (Lycium barbarum) is an oval-shaped orange-red fruit with a slightly sweet flavor. This berry has a high antioxidant potential and presents interesting nutritional and therapeutic properties. Irradiation is a safe method that has long used to reduce the microbiological contamination of dried and dehydrated food products. This study aimed to evaluate the irradiation effects on the bioactive compounds of goji berries by irradiating samples with an electron beam at doses of 2.5, 5.0, 7.5, and 10.0 kGy. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity and oxygen radical absorbance capacity (ORAC) were assessed by analyzing the hydroalcoholic extracts. The total phenolic compounds determined by the Folin-Ciocalteu assay. Doses up to 10.0 kGy did not significantly affect the antioxidant activity in the DPPH assay, and electron beam irradiation improved the total antioxidant activity of the samples in ORAC, as well as a total flavonoid and phenolics assays.
Effect of ionizing radiation on traditional and bacon “farofa”
2021 - SÁ, ANA P.N. de; NEGRAO, BIANCA G.; NABESHIMA, ELIZABETH H.; KOIKE, AMANDA C.R.; VILLAVICENCIO, ANNA L.C.H.
Seasoned “farofa” is a typical Brazilian dish made with toasted cassava flour. It is known that ionizing radiation is widely employed to improve food products extending its shelf life. In this context, this work analyzes the effects of ionizing radiation on the rheological and physicochemical properties of bacon (BF) and traditional (TF) “farofa”. The samples were obtained from local markets (São Paulo/Brazil) and irradiated in the electron beam accelerator of Nuclear and Energy Research Institute (IPEN/CNEN-SP, São Paulo, Brazil) in doses of 1, 5 and 10 kGy, and analyzed on the first, fifteenth and thirtieth storage day. The results showed that the irradiated TF and BF samples displayed acidic pH throughout the storage period, regardless of dose increase. The different irradiation doses did not significantly affect (Tukey test p > 0.05) the water activity, keeping the products in the safe range. The colorimetric analysis, also showed no significant difference (p ≥ 0.05) among the samples and ionizing radiation doses, indicating that the yellowish coloration remained stable throughout the experiment. After the thirtieth day, the moisture of the TF and BF samples ranged from 7.06% to 9.75%. Irradiation had a significant impact on the viscosity profile and texture characteristics of the “farofa”, such as hardness, cohesiveness, and the springiness at 5 and 10 kGy.
Analise microbiológicas de fermento biológico seco processado por radiação gama
2018 - SABUNDJIAN, INGRID T.; NEGRAO, BIANCA G.; SÁ, ANA P.N. de; VILLAVICENCIO, ANNA L.C.H.
O objetivo do trabalho foi identificar se houve alteração no crescimento de UFC em placa e na viabilidade de leveduras e bactérias totais em fermento biológico seco tratado por diferentes doses de radiação gama e determinar a dose D10 para bactérias Totais e Leveduras a fim de analisar se o processamento promoveu algum benefício sem causar inviabilidade do mesmo. As diferentes amostras de fermento biológico foram irradiadas com doses de 0 (controle); 0,5; 1; 2 e 3kGy no Centro de Tecnologia das Radiações do (IPEN/CNEN – SP) em fonte de 60Co (Gammacell-220), com taxa de dose de 3,51kGy/h. O aumento da dose de radiação provocou uma diminuição na contagem de UFC de leveduras e de bactérias totais, bem como, na frequência de células viáveis de leveduras. A dose de radiação necessária para eliminar 90% da população leveduriforme ficou entre 1,10 e 2,23kGy e para a população bacteriana variou entre 2,31 e 2,95kGy. Nos resultados são demonstrados claramente os pontos negativos da aplicação de radiação ionizante em fermento biológico seco, pois o intervalo de D10 encontrado para bactérias totais é superior ao encontrado para leveduras. Sendo assim, torna-se inviável a utilização deste recurso para a melhora da qualidade do produto.
Ionizing radiation as a phytosanitary treatment in Zea mays L. var. everta
2018 - NEGRAO, BIANCA G.; VILLAVICENCIO, ANNA L.C.H.; ARTHUR, VALTER
Quarantine treatment has been used to provide protection against pests by eliminating the stages evolutionary cycle that may be associated with the product. Popcorn is used in the food industry in the form of dry beans and is an economic option for producers and their sale generates foreign exchange worldwide. The aim of this work is to evaluate the effects of gamma radiation on seeds of popcorn (Zea mays L. var. everta). The seeds was be purchased in the retail trade of São Paulo city, later was be infested in the Laboratory of Food Irradiation at IPEN, then was be submitted to different doses of gamma radiation: 0, 25, 50, 75 and 100 Gy. A Gammacell-220 at 60Co source at a dose rate of 1.4 kGy / hr at the Technology Center of Radiation (CTR) IPEN-CNEN / SP. Each treatment was consisting of 3 replicates with 100 grams of popcorn kernels. Samples was be packed and stored at a temperature of 25 ± 2 ° C and relative humidity of 70 ± 5%. By the results, it can conclude that the dose of 75 Gy can be indicated to control insects Sitophilus zeamais in popcorn.
Use of medium and high doses of ionizing radiation in cassava starch
2017 - NEGRAO, BIANCA; VILLAVICENCIO, ANNA L.C.H.
Cassava starch is susceptible to microbial changes, which can affect its shelf life. Ionizing radiation is used in doses sufficient to reduce microorganisms. However, when being exposed to starch granule it can cause changes in structure when compared to other methods. The aim of the work was to irradiate starches with medium and high doses in order to observe the effects on color of cassava starch and texture of tapioca. The starch samples were purchased in supermarkets in two types: starches with preservative (Fc – sodium metabisulphite) and starch without preservative (Fs). They were then irradiated at doses of 0, 5, 10 and 15 kGy in a 60Co Gamma cell 220 with dose rate of 0.764kGy/h-1. Subsequently, the samples were evaluated on 1st, 15th and 30th day after irradiation. The results show that in relation to the tonality (h°) there was a significant difference (P> 0.05) between the control and the irradiated samples on the 30th day. It's worth mentioning that, Fc and Fs had similar behavior at dose of 10 kGy, showing averages nearly h= 90º (Fs: 90.6±0,4; 90.0±0,3; 90.4±0,50 and Fc: 90.5±0,3; 89.9±1,6; 89,5±2,9 on the 1st, 15th and 30th day, respectively) indicating that the samples turned yellowed according to storage days. This tendency towards the yellow color can be attributed to the modification of reactions of the starch monosaccharide’s. The radiation can influence the rupture of surface the starch. For this reason, the tapioca showed increase in hardness (N) according to the increase of radiation dose as well as with storage days. However, on the 30th day, Fc showed no significant difference between doses of 5kGy (10.06); 10kGy (11.44) and 15kGy (11.67). The analysis of the results allows concluding that in this product it's possible use doses up to 10kGy because not change considerably the parameters studied.
Application of ionizing radiation in the coconuts of “Licuri” Syagrus Coronata (mart.) in the control of the Pachymerus Nucleorum beetle (Fabricius, 1792)
2019 - NOGUEIRA, ALEXANDRA G.; NEGRAO, BIANCA G; ARTHUR, VALTER; VILLAVICENCIO, ANNA L.C.H.
The “licuri” fruit palm (Syagrus coronata) is harvested extractively from palm trees that grow in native forests, pastures, and in association with other cultures being part of the regional economy in the hinterland of Bahia. Among the problems related to the “licuri” palm is the insect specie Pachymerus nucleorum (Fabricius, 1792), which stands out as a pest of economic importance because it causes direct damages to the “licuri's” almonds. Due to this the objective of the work was to determine the lethal doses of gamma radiation for the phases of egg, larvae and pupae of Pachymerus nucleorum aiming at its control in almonds of coconuts of licuri. A Cobalt 60 irradiator, Gammacell-220 type, installed at the Institute of Energy and Nuclear Research - IPEN / CNEN, located in São Paulo / Brazil, at a dose rate of 748 Gy / h, was used to carry out the research with the Pachymerus nucleorum biological cycle. Each treatment for all stages of egg, larvae and pupae inside of coconuts consisted of 3 replicates with 12 coconuts each, in a total of 216 coconuts per test that were irradiated in the following doses: 0 (control), 50, 75, 100 and 125 Gy. After irradiation, the samples were stored at a temperature of 25 ± 5 ° C and relative humidity of 70 ± 5%, where the development of egg, larvae and pupae stages until adult transformation in irradiated almonds. From the results obtained it is concluded that the dose of 125 Gy was sufficient to interrupt the development of the phases of the insect. Therefore, this dose of radiation can be used for quarantine treatment of the immature stages of Pachymerus nucleorum in the coconuts of “licuri” Syagrus coronata palm.