RAFAEL DA SILVA COSTA
2 resultados
Resultados de Busca
Agora exibindo 1 - 2 de 2
Resumo IPEN-doc 28450 A comparison between two populations of mesenchymal stem cells that can improve the osteogenesis imperfecta mouse phenotype2021 - GOMES, ALISSANDRA de M.; JESUS, GUSTAVO P.P. de; ZACARIAS, ENIO A.; YOSIDAKI, VANESSA L.; LIMA, DARLE B. de; COSTA, RAFAEL S.; RANGEL, ERIKA B.; SOUSA, POLIANA E.S.; SILVA, CHRISTIAN S.; BARTOLINI, PAOLO; PERONI, CIBELE N.Osteogenesis imperfecta (OI) is an inherited disease characterized by fragility, deformity and low bone density, besides other clinical manifestations. Type-I OI is the mildest and most common form of the disease, caused by a mutation in the COL1A1 gene and resulting in half normal-collagen production. Our purpose was to compare mesenchymal stem cells from bone marrow (BM-MSCs) with those from adipose tissue (AD-MSCs), both used for improving heterozygous oim mice phenotype, similar to human type-I OI. Mice were irradiated and cells injected into femoral condyles, bone mineral density (BMD) and femoral length were measured by X-ray, at the beginning and end of the assay. Femurs and quadriceps allowed bone fragility evaluation by biomechanical test and collagen Col1a1 and Col1a2 quantification via ELISA. BMD showed no significant difference between the groups, while femur length variation was higher in the BM-MSCs group, compared with the control (P=0.0301). Fragility was improved with AD-MSCs when flexion extension to fracture (P=0.0028) and time to fracture (P=0.0032) were evaluated. There was no significant difference in the maximum load supported by femurs until fracture. An increase in Col1a1 concentration with BM-MSCs in comparison with AD-MSCs (P=0.0281) was obtained. Although no significant difference in Col1a2 concentration was observed between the groups, a higher expression level was obtained with AD-MSCs. We can thus conclude that AD-MSCs were more efficient than BM-MSCs for improving bone quality in type-I OI.Resumo IPEN-doc 26613 Comparação da eficiência entre vetores contendo a sequência complementar com ou sem a região downstream (3') do hormônio de crescimento humano em células HEK-2932019 - COSTA, RAFAEL da S.; PERONI, CIBELE N.