RODRIGO GUIMARÃES QUEIROZ

RODRIGO GUIMARÃES QUEIROZ

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State of the art and current advances on protein cross-linking by irradiation: protein based nanocarriers and bioactive nanoparticles
2017 - VARCA, G.H.C.; MOHAMED, L.B.; FAZOLIN, G.N.; BATISTA, J.G.D.S.; QUEIROZ, R.G.; LUGAO, A.B.; NAVARRO MARQUES, F.A.; FERREIRA, A.H.
The highlighted role of protein and peptide based delivery systems relies upon the possibility to develop biocompatible drug carriers featuring site specific delivery, biological affinity among unique advantages. Recently, a technique for protein nanostructuring by the use of radiation has been recently reported by our group. Advantages of the use of radiation over conventional methods are related to the possibility to achieve protein cross-linking and sterilization in a single step, as well as the capacity to allow the design of nanocarriers without the need of monomers or toxic cross-linkers. This work reports the use of high energy irradiation towards the design of size-controlled protein-based nanocarriers and bioactive nanoparticles, using bovine serum albumin (BSA) and papain as model protein and protease, respectively, including the state of the art and current advances of the technology. The technique implies on protein desolvation/solvation techniques followed by cross-linking by EB radiation or -irradiation alone, although nanoparticles were also achieved in absence of the cosolvents. Size-controlled BSA nanocarriers were manufactured up to 80 nm and papain bioactive nanoparticles up to 12 nm, as determined by dynamic light scattering. Nanocarrier morphology was evaluated by and negative staining transmission electron microscopy. Protein cross-linking and changes in aromatic the amino acids were evaluated by fluorescence measurements. Biocompatibility experimentswere also performed by means of cytotoxicity and cytokines production. The potential of the systems for the delivery of radiopharmaceuticals or chemotherapeutic agents were also assayed, using technetium or Paclitaxel respectively. In conclusion, the technique allowed the production of biocompatible and bioactive protein nanoparticles suitable for the administration of radiopharmaceuticals and chemotherapeutic agents.
In vitro and in vivo antitumor effects of Azidothymidine in a human multiple myeloma cell line
2012 - LEVY, DEBORA; RUIZ, JORGE L.; BROCARDO, GRACIELA; FERREIRA, ADILSON; QUEIROZ, RODRIGO; MARIA, DURVANEI; BYDLOWSKI, SERGIO; PEREIRA, JULIANA
Azidothymidine (AZT) is an antiretroviral nucleoside analogous inhibitor of reverse transcriptase with known effects on cell proliferation, apoptosis, and angiogenesis Multiple myeloma is a severe disease and one of the steps involved in the malignant transformation of plasma cells is the activation of the nuclear factor kappa B (NF-κB) pathway.
Radiation-synthesized protein-based drug carriers: Size-controlled BSA nanoparticles
2016 - QUEIROZ, R.G.; VARCA, G.H.C.; KADLUBOWSKI, S.; ULANSKI, P.; LUGAO, A.B.
Irradiation as an alternative route for protein crosslinking: Cosolvent free BSA nanoparticles
2016 - VARCA, GUSTAVO H.C.; QUEIROZ, RODRIGO G.; LUGAO, ADEMAR B.
Radiochemical and biological evaluation of Ter-Cys-RGD derivarive labeled with the precursor [sup(99m)Tc(Hsub(2)O)sub(3)(CO)sub(3)]sup(+)
2007 - TEODORO, RODRIGO; FAINTUCH, BLUMA L.; QUEIROZ, RODRIGO G.; MURAMOTO, EMIKO; MORGANTI, LIGIA; NASCIMENTO, NANCI do
sup(99m)Tc labeling of annexin fragment by two different techniques for detection of apoptosis in cancer
2007 - FAINTUCH, BLUMA L.; TEODORO, RODRIGO; QUEIROZ, RODRIGO G.; MURAMOTO, EMIKO; CHURA-CHAMBI, ROSA M.; MORGANTI, LIGIA; NASCIMENTO, NANCI
sup(99m)Tc-Hynic-RGD analog for monitoring of integrin expression in ischemic animal model
2007 - QUEIROZ, RODRIGO G.; FAINTUCH, BLUMA L.; TEODORO, RODRIGO; MURAMOTO, EMIKO
Componentes derivados de venenos de serpentes com ação antitumoral em células de melanoma murino
2012 - QUEIROZ, RODRIGO G.
Apesar dos constantes avanços no tratamento de câncer, essa doença continua sendo umas das principais causas de mortalidade no mundo todo, portanto, é imperativo que novas modalidades de tratamento sejam desenvolvidas. Venenos de serpentes causam uma variedade de efeitos biológicos, pois constituem uma mistura complexa de substâncias como disintegrinas, proteases (serino e metalo), fosfolipases A2, L-aminoácido oxidases entre outras. No presente trabalho pesquisou-se em alguns venenos de serpentes frações com atividade antitumoral, pois há relatos de compostos ofídicos que apresentam esta atividade. Após fracionamento de venenos de serpentes das famílias Viperidae e Elapidae foram feitas incubações das frações obtidas com células normais de fibroblasto L929 e de melanoma B16-F10. Os resultados mostraram que as frações do veneno da serpente Notechis ater niger apresentaram a maior especificidade e potencial antitumoral em células de melanoma murino B16-F10 que os demais venenos utilizados. Este achado, aliado ao fato de ser um veneno pouco explorado foram determinantes para que este veneno fosse selecionado para dar continuidade aos estudos. As frações citotóxicas deste veneno foram submetidas a análises para identificar e caracterizar os componentes que apresentaram esta atividade atitumoral. Ensaios de western-blot e zimografia sugerem que estas proteínas não pertencem à classe das metalo e serinoproteinases.
Evaluation of sup(99m)Tc-neurotensin analog using different chelators
2008 - FAINTUCH, BLUMA; TEODORO, RODRIGO; QUEIROZ, RODRIGO; FAINTUCH, SALOMAO
Initial studies of radiolabeling and biodistribution of peptide p160 analog with technetium-99m
2008 - QUEIROZ, RODRIGO; FAINTUCH, BLUMA; FAINTUCH, SALOMAO; TEODORO, RODRIGO