ALEXANDRE LUIZ DE SOUZA

ALEXANDRE LUIZ DE SOUZA

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Variations in essential elements after malignant transformation of kidney epithelial tubular cells
2020 - BELLINI, MARIA H.; SOUZA, ALEXANDRE L. de; SILVA, FABIO F. da; GUILHEN, SABINE N.; FERREIRA, RAFAEL V. de P.; ARAUJO, LEANDRO G. de; OLIVEIRA, JOAO E. de; MARUMO, JULIO T.
Cell line-based research is a valuable tool for the study of cancer physiopathology and the discovery of new drugs for use in clinical practice. In this study, inductively coupled plasma mass spectrometry (ICP-MS) was used to estimate Ca, Co, Cu, Fe, K, Mg, Mn, Na, P, S, Se, and Zn in epithelial tubular cells (HK-2) and kidney tumor cells (Caki-1 cells). The most relevant difference was a decrease in the contents of Ca, Cu, Fe, K, Mg, Mn, Na, P, S, and Zn. A significant accumulation of Co was also detected in Caki-1 cells. The fold change variation of each element concentration between HK-2 and Caki-1 cells was Ca (‒0.40), Co (1.37), Cu (‒0.68), Fe (‒0.56), K (‒0.40), Mg (‒0.41), Mn (-0.54), Na (‒0.33), P (‒0.31), S (‒0.26), and Zn (‒0.73). These findings indicate that the elements mainly affect the metabolic pathways of epithelial kidney cells. Thus, our findings open a new avenue for RCC target therapy
Essential elements as biomarkers of metastatic renal cell carcinoma
2020 - BELLINI, MARIA H.; SOUZA, ALEXANDRE L. de; SILVA, FABIO F. da; GUILHEN, SABINE N.; FERREIRA, RAFAEL V. de P.; OLIVEIRA, JOAO E. de; MARUMO, JULIO T.
Renal cell carcinoma (RCC) represents 3% of human malignant tumors and approximately 90% of malignant renal neoplasms. Despite great therapeutic advances in the last decade, metastatic RCC (mRCC) is still considered an incurable disease. In this study, we examined the potential of essential elements as biomarkers of mRCC using an orthotropic metastatic mouse model. Frozen lung and plasma samples from healthy and mRCC-induced mice were lyophilized, digested, and analyzed using inductively coupled plasma mass spectrometry. In metastatic lungs, a significant increase in Ca concentration (268%) was observed, whereas a significant decrease in Cu (23.2%), Fe (17.4%), Mn (38.8%), and Na (11.7%) was observed. The plasma of mRCC-induced mice showed decreased concentrations of Mn (53%), Na (19.7%) and Zn (49,50%) and increased levels of Ca (53%), Cu (39.5%), Our findings revealed marked differences in the concentrations of essential elements in the lung and plasma of the metastatic mouse model. The circulating levels of Ca, Cu, Mn, Na, and Zn could be utilized as diagnostic and therapeutic response biomarkers.
Evaluation of analytical procedures for the determination of cadmium, boron and lithium in UAlx samples by inductively coupled plasma optical emission spectrometry (ICP OES)
2013 - GUILHEN, SABINE N.; SOUZA, ALEXANDRE L. de; KAKAZU, MAURICIO H.; COTRIM, MARYCEL E.B.; PIRES, MARIA A.F.
Mikrobohren mit ECM und thermischem materialabtrag
2010 - SOUZA, ALEXANDRE; ROSSI, WAGNER de
An overview of spectrometric techniques and sample preparation for the detrmination of impurities in uranium nuclear fuel grade
2013 - SOUZA, ALEXANDRE L. de; COTRIM, MARYCEL E.B.; PIRES, MARIA A.F.
This review is focused on the impurities determination in uranium nuclear fuel grade matrix with the use of spectrometric methods. It is known that uranium is the major element and material for the sustainable use in the nuclear energy. The optimized use of this technology involves the improvement of several processes to power generation. With regard of fuel reliability, the chemical characterization combined with environmental protection is particularly important in the uranium production cycle or in the nuclear fuel cycle, subject to an increasing number of international conventions and protocols. The performance of nuclear fuel in reactors depends mainly on the chemical composition of its impurities and its estimation is important from the point of view of neutron economy. Among the instrumental analytical techniques commonly used for the determination of elemental impurities in uranium compounds spectrometric techniques are commonly used, such as; FAAS, GFAAS, ICP OES and ICP-MS. Usually, it is necessary in a previous uranium separation and preconcentration of the impurities for elemental determination. Thus, the aim of this review is to give an overview of the main process using sample preparation, separation and preconcentration of analytes for the instrumental determination.