CLAUDIA BIANCHI ZAMATARO

CLAUDIA BIANCHI ZAMATARO

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In vivo study of diode laser 940 nm operating in high intensity mode in the periimplantitis disease
2018 - GOTTARDI, MARIELLA da S.; KAWAMOTO, DIONE; ZAMATARO, CLAUDIA B.; ANA, PATRICIA A. da; MAYER, MARCIA P.A.; ZEZELL, DENISE M.
The evolution of periimplantitis has been associated with several risk factors. The standard treatment consists on subgingival scaling and blasting with sodium bicarbonate. This 6 months longitudinal clinical study evaluated and compared two treatment methods: conventional as described and complemented by irradiation with high intensity diode laser (940 nm, Biolase) aiming to establish a more efficient periimplantitis management. After approval by the Human Research Ethics Committee, 20 patients of both sexes, with implants in function for at least 12 months and with diagnosis of periimplantitis were selected and randomly distributed in two experimental groups. Ten of them were conventionally treated as control group, and the remaining ten were in the laser treated group (1W, 4.24kJ/ cm2 energy density, pulsed mode, 30s, twice). There were six sites for treatment standardization: vestibular region (mesial, central and distal thirds) and buccal region (mesial, central and distal thirds). Results has shown that overall clinical evaluation has no significant difference between groups control and treatment. less bleeding and suppuration, as shallower periodontal pocket indicated a decreasing tendency on laser treated group. Quantitation of total bacteria and Porphyromona gingivalis by real-time PCR shown that laser synergy is a complementary therapy that could act as an additional option to the conventional treatment in difficult response cases.
Effect of Nd:YAG laser on caries prevention of dental enamel by optical attenuation coefficient with optical coherence tomography (OCT)
2018 - MORAES, MARCIA C.D.; PEREIRA, DAISA L.; ZAMATARO, CLAUDIA B.; ZEZELL, DENISE M.; FREITAS, ANDERSON Z.
Caries is a multifactorial disease, caused by the continuing loss of hydroxyapatite crystals. The association of topical acidulated phosphate fluoride application (APF) and high intensity lasers has the potencial to increase the resistance to demineralization of dental tissues. The Nd:YAG laser, specially associated to a photoabsorber over the dental surface, may induce thermal changes in order to increase the resistance to demineralization. Optical Coherence Tomography (OCT) is a non-ionizing radiation imaging technique that can be performed in real time in vivo and can detect early changes in the optical properties of t issues. This study aimed to characterize the effectiveness of the association of Nd:YAG la ser and APF gel in the prevention of caries lesions in human dental enamel in vitro, due to structural changes in function of the pH cycling, the treatments performed as well as to evaluate the potential of OCT to diagnose early demineralization. 160 human enamel samples were divided in four groups and submitted to pH cycling during 20 days, after the following treatments: G1- untreated; G2- treated with APF gel (1.23% F-, 4min); G3- irradiated with Nd:YAG (1064nm, 84.9J/ cm2, 10Hz) followed by APF application and G4- APF application before Nd:YAG laser irradiation (same conditions of G3). OCT (930 nm) analysis were performed before and after the treatments, 5th, 10th, 15th and 20th day of pH cycling. The optical attenuation coefficient decreased significantly (p<0.05) as demineralization increased over time and better results were achieved in group G4. OCT technique can diagnose early demineralization in enamel.
Longevity of diode laser irradiation in dentine
2018 - PEREIRA, DAISA L.; MORAES, MARCIA C.D.; ZAMATARO, CLAUDIA B.; ZEZELL, DENISE M.
The efficacy of pulsed infrared lasers on clinical dentin wear sensitivity is well stablished in the Literature. This work aims to evaluate the effect of diode laser combined with fluoride products regarding the longevity of the sensibility treatment under the effect of the domestic use products. After Ethics Committee approval, 75 slabs of bovine root dentin measuring 8 mm2, were randomized into 5 groups (n=1 5): G1- untreated; G2- treated with acidulated phosphate fluoride (APF-gel, [F-J=1.23 %, pH=3.3 to 3.9); G3- irradiated with diode laser (980 nm, 2.5W, 5ms, 25Hz) using a cool paste as photoabsorber; G4- irradiated with diode laser (same conditions of G3) using a coal paste as photoabsorber + application of APF-gel and G5- application of APF-gel+ diode laser irradiation (same conditions of G3). All samples were submitted to an erosive and abrasive demineralization (Sprite Zero, pH=2.8, 90s, 4x/day), and remineralization (artificial saliva, pH=7.4) cycling for 10 days. Twice a day, slabs were brushed for 15s using a whitening dentifrice (Sensodyne True White, GSK, EUA). Slabs were evaluated by Optical Coherence Tomography (OCT) at the initial and 7th day of cycling. OCT measurements found a significant increase on dentin wear depth and area of lesions with the increase of cycling days. G1 and G2 hod significant higher mineral loss compared to the other groups. G3, G4 and GS showed no significant differences between them. Results suggested that the sensibility of the treatment with the diode laser has longevity despite the high abrasivity of dentifrice used in maintenance.
Gamma sterilized human dental enamel submitted to Er,Cr: YSGG laser irradiation associated to fluoride for in situ model
2018 - ZAMATARO, CLAUDIA B.; PEREIRA, DAISA de L.; LIMA, CASSIO A.; SCAPIN, MARCOS A.; ZEZELL, DENISE M.
Nd:YAG laser irradiation of darkened dental enamel by means of dentrifice use
2018 - PEREIRA, DAISA L.; GOMES, GABRIELA V.; SUGAHARA, VANESSA M.L.; SCAPIN, MARCOS A.; ANA, PATRICIA A.; ARANHA, ANA C.C.; ZAMATARO, CLAUDIA B.; ZEZELL, DENISE M.
As the world population is growing older, dental erosion became a real concern on dentistry. Optical Coherence Tomography and X-Ray fluorescence were used to analyze enamel samples covered by black dentifrice as photoabsorber irradiated with NdYAG laser, then submitted to an erosive cycling. Heat injury risk in deeper tissue decreases because of the dentifrice dark pigment, when the most of photons are absorbed in the surface. Sixty Bmm2 blocks of bovine enamel, were randomized into 4 groups: G1- untreated; G2- treated with acidulated phosphate fluoride (APF, [FJ::1.23%, pH::3.3 to 3.9); G3- irradiated with Nd:YAG laser (1064nm, 0.6W, 10Hz- Lares Research®) using a coal paste as photoabsorber; G4- irradiated with Nd:YAG laser (1064nm, 0.6W, 10Hz), using a black dentifrice (Black is White, Curaprox®). All samples were submitted to a 3-day erosive demineralization (Citric acid 1%, pH::3.6, 5min, 2x/day) under agitation, and remineralization cycling (artificial saliva, pH::7, 120minJ. After treatments and pH cycling, the [Ca]/[P] ratio decreased in the laser irradiated groups. The samples were evaluated by OCT before and after treatment and after erosive cycling. OCT measurements found smaller lesion depths in laser irradiated groups than in the other groups and there were statistically significant differences between G3 and G4 groups (p<0.05). Heating promoted by Nd:YAG laser irradiation changed the morphological and crystallographic enamel surface properties and has shown to be a promissory alternative to prevent erosion.
SEM/EDS for determining the phosphorus and calcium content in human dentin submitted to radiotherapy for head and neck cancer
2020 - SCAPIN, MARCOS A.; MOREIRA JUNIOR, CLAUDIO; ZAMATARO, CLAUDIA B.; GONCALVES, SERGIO E. de P.; ZEZELL, DENISE M.
The research followed the CONSORT 2010 guidelines. Patients who underwent radiotherapy due to the head and neck cancer (n = 12) and healthy patients (n = 12) donated the teeth. Twenty-four urgently extracted teeth for periodontal reasons or in order to prevent infectious processes were used. All teeth obtained had the approval from the ICT - UNESP Human Experiments Ethics Committee. The teeth obtained were separated into two large groups: Control (n = 12 dentin teeth of healthy patients) and Irradiated (n = 12 dentin teeth of patients irradiated in vivo) and sectioned in their long axes, obtaining two halves (n = 24 per group). The samples were analyzed by Scanning Electron Microscopy with Energy Dispersive (SEM/EDS) and Surface Microhardness (SMH). The results obtained by SEM/EDS and X-Ray Fluorescence techniques were analyzed separately and then compared to each other. To validate the analytical method of the correlation between the two types of tests performed, the Horwitz equation was used, which would generate a Horwitz value and establishes for either method. Precision for repeatability was assessed using the relative percentage standard deviation (RDS%) and the acceptability criterion by the Horwitz test. Accuracy was assessed by relative percentage error (ER%) and acceptance criterion by z-score. The precision for repeatability was evaluated through the relative standard deviation (RSD%) and the acceptability was verified using the equation of Horwitz, in which the HORRAT’s (HOR) values fit more accordingly.
Evaluation of the anti-caries effect beyond the critical enamel pH of preventive treatment of fluoride associated with Nd:YAG laser irradiation
2021 - JUVINO, AMANDA C.; ROMANO, MARIANA; ALVES, NATHALIA Z.; FREITAS, THAIS R.; ZAMATARO, CLAUDIA B.; ZEZELL, DENISE M.
This study aimed to evaluate the anti-caries effect of fluoride associated with Nd:YAG laser irradiation in the h-eatment of enamel Eight groups (n = S) were analyzed: Negative Control pH 4.5; Negative control pH 4; Fluoride pH 4.5; Fluoride pH 4; Nd-YAG pH 4.5; Nd-YAG pH 4; (Fluoride + Nd-YAG) pH 4.5 and (Fluoride + Nd-YAG) pH 4. All samples were analyzed by Scanning Electron Microscopy (SEM) before and after the cycle. Quantification of phosphorus in the cycling solutions was carried out using the colorimetric method, as an indication of enamel demineralization. The anti-caries effect of the Fluoride + Nd: YAG treatment in addition to the critical pH of the enamel can be observed in the morphological analysis, however in the analysis of the demineralization solutions, the amount of phosphorus showed a difference only in the Fluoiide group compared to the others in the investigative pH.
Analysis of ceramic laminates removal with Er,Cr:YSGG laser by optical coherence tomography
2019 - ZANINI, NATHALIA; ZAMATARO, CLAUDIA B.; RABELO, THAIS F.; JUVINO, AMANDA C.; KUCHAR, NIELSEN G.; CASTRO, PEDRO; ANA, PATRICIA da; ZEZELL, DENISE
Porcelain laminated veneers have been widely used. For wear of hard tissue such as enamel and dentin, the diamond rotary instrument is the most traditional, but the laser has become recently used to remove aesthetic facets. Optical coherence tomography (OCT) used as an optical biopsy, is important for morphological analysis and attenuation coefficient is related to the property of the photons to be scattered by the samples. After approval by the Ethics Committee, the present study investigated the detachment of 30 ceramic E-max fragments cemented in human dental enamel of dimensions 3mm x 3mm x 0.7mm with 3 types of resin cements, RelxY Veneer, Relx U200 and Variolink Veneer. The samples (Enamel + Ceramic Fragment) were randomly distributed in the 3 groups and cemented according to the manufacturer. After that, they were prepared for irradiation with the Er,Cr: YSSG laser under predetermined conditions (3.5 and 3W, 20Hz, 60% water and 40% air flow). OCT analysis was done before and after irradiation. We observed that themorphological changes of the enamel surface showed an increased surface area due to the cement remaining in the enamel.We concluded that the Er, Cr: YSGG laser, when used in the irradiation protocol tested, seems to be a safe tool for the removal of laminates.
Removal of laminates with Er,Cr:YSGG laser from dental enamel submitted to gamma radiation
2019 - RABELO, THAIS F.; ZAMATARO, CLAUDIA B.; ZANINI, NATHALIA; JUVINO, AMANDA C.; KUCHAR, NIELSEN G.; CASTRO, PEDRO A.A.; ZEZELL, DENISE M.
Background: Modern Dentistry is characterized by the search for aesthetic perfection in the smile. Many efforts are made regarding procedures related to manufacturing, cementing, maintenance and removal of ceramic laminates. The laser removal of laminates has become more frequent when the aesthetical procedures needs to be replaced. On the other hand, the head and neck cancer treatment causes comorbidities in the buccal environment when laminates are in place. Objective: To evaluate in vitro the removal of ceramic fragments by means of Er,Cr:YSGG laser irradiation, after gamma radiation. Methods: 20 Lithium disilicate veneers were cemented with Variolink to human dental enamel and then samples were irradiated with 0.07 kGy. After gamma irradiation, the laminates removal with Er,Cr:YSGG laser was performed. The control group was not subjected to gamma radiation. Dental enamel samples were analyzed by Scanning Electron Microscopy (SEM), Fourier Transformed Infrared Spectroscopy (FTIR) and Surface Microhardness Loss (SMH). Results: SEM has shown that less cement was found after removal of the facets in gamma irradiated group when compared to the control. For both groups there was alteration of the SMH possibly due to the use of the adhesive system. When performed intra group analysis, the sample being its own control of baseline in the FTIR analysis, there were no band shifts or formation of new compounds on the surface of human dental enamel. Conclusions: The Er,Cr:YSGG laser is an effective alternative for laminates removal in human dental enamel even when it is subjected to gamma radiation.
Calcium analysis of gamma sterilized human dentin submmited to Er,Cr:YSGG laser irradiation
2019 - KUCHAR, NIELSEN G.; ZAMATARO, CLAUDIA B.; RABELO, THAIS; JUVINO, AMANDA; ZANINI, NATHALIA; CASTRO, PEDRO; ZEZELL, DENISE M.
Studies report that gamma radiation changes the oral cavity of patients submitted to radiotherapy. These changes include xerostomia, resulting in an unsaturation of calciumand phosphate in the oral cavity. However, human enamel samples isolated from the altered oral environmentwhen irradiatedwithgamma radiation at doses of up to 25kGy (much higher than radiotherapy doses) were shown to have undergone no changes in molecular hardness or micro surface. After approval of the Research Ethics Committee, 20 human dentin samples were evaluated before and after irradiation, split randomly into control group (n = 10) and irradiated group (n = 10) treated with 25.0 kGy at the Co60 multipurpose irradiator and irradiated with Er,Cr:YSGG laser in the parameters: 8.5 J/cm2 in the Fourier Transform Infrared Spectroscopy (FTIR), % surface Microhardness loss and Scanning Electron Microscopy (SEM). At the end, acidic biopsies were performed to quantify the concentration of calcium present in the samples. In the results of FTIR analysis differences were found only in the bands of organic content and in the inorganic content, difference were not found between before and after irradiations. The EDS and % Surface Microhardness loss analysis corroborates these findings, as well as no significant loss of the Calcium content before and after their radiation with Co60 at 25 kGy and with the Er,Cr:YSGG laser. These findings lead us to a new hypothesis of behavior of the hydroxyapatite crystal submitted to the gamma irradiation.