SABRINA METAIRON
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Artigo IPEN-doc 26908 Determination of inorganic elements in blood of mice immunized with Bothrops Snake venom using XRF and NAA2015 - SILVA, L.F.F.L. da; ZAMBONI, C.B.; BAHOVSCHI, V.; METAIRON, S.; SUZUKI, M.F.; SANTANNA, O.A.; RIZZUTTO, M.A.In this work, mice genetically modified [HIII line] were immunized against different Bothrops snake venoms to produce anti-Bothrops serum (antivenom). The Neutron Activation Analysis (NAA) and Energy Dispersive X-Ray Fluorescence (EDXRF) techniques were used to evaluate Ca and Fe concentrations in blood of these immunized mice in order to establish a potential correlation between both phenotypes: antibody response and blood constituents after Bothrops venom administration. The results were compared with the control group (mice not immunized) and with human being estimative. These data are important for clinical screening of patients submitted to immunological therapy as well as the understanding of the envenoming mechanisms.Artigo IPEN-doc 25900 Ions concentration in blood samples of SJL/J dystrophic mice strains using X-ray fluorescence spectrometry2019 - GIOVANNI, D.N.S.; ALMEIDA, M.R.; ZAMBONI, C.B.; METAIRON, S.; BALDUINO, K.N.; SUZUKI, M.F.; BUENO JUNIOR, C.R.Star This study proposes an investigation of ions in whole blood of the dystrophic animal model SJL/J (mice strain with dysferlin protein deficiency) and in the control group (C57BL/6J) using the Energy Dispersive X-ray Fluorescence (EDXRF) spectrometry technique. The comparison between control and dystrophic mice results shown an increase in blood for P, S, K and Fe (p < 0.05) while a decrease in Ca (p < 0.05). This elemental analysis will contribute to evaluate the best diagnostic, care and treatment procedures, for the Progressive Muscular Dystrophy.Livro IPEN-doc 24360 Dosagem de íons em tecido biológicos de camundongos distróficos2017 - METAIRON, SABRINA; ZAMBONI, CIBELE B.Nesta obra a investigação multielementar de tecidos biológicos (sangue total, ossos e órgãos) de camundongos distróficos, usados como modelo animal da Distrofia Muscular de Duchenne (DMD), foi realizada utilizando a técnica de Análise por Ativação com Nêutrons (AAN). O objetivo teve como foco determinar a concentração de íons de relevância clínica e nutricional (Br, Ca, Cl, K, Mg, Na e S) de camundongos da linhagem distrófica Dmdmdx/J e grupo controle C57BL/6J. Amostras de sangue total, ossos, coração e músculo foram irradiadas no reator nuclear IEA-R1 no IPEN (São Paulo, Brasil). Os resultados constituem as primeiras estimativas para os valores de referência nesses tecidos biológicos e viabilizam a monitoração de diversas funções do organismo saudável (C57BL/6J) e distrófico (Dmdmdx/J). Este livro apresenta dados que poderão auxiliar pesquisadores avaliar e comparar as vantagens e desvantagens dos diferentes tratamentos, realizados na distrofia muscular, quando este modelo animal for empregado, auxiliando na avaliação da eficácia de novos procedimentos terapêuticos antes de serem empregados em pacientes com DMD.Artigo IPEN-doc 24352 Iron determination in whole blood samples of dystrophic mouse strains using X-Ray fluorescence spectrometry2017 - METAIRON, SABRINA; ZAMBONI, CIBELE B.; GIOVANNI, DALTON N. da S.; SUZUKI, MIRIAM F.; BUENO JUNIOR, CARLOS R.; RIZZUTTO, MARCIA de A.Several diseases can be monitored by assessing the variation of specific ion concentration in body fluids. This study proposes an alternative analysis, based on the X-ray fluorescence spectrometry technique, for the investigation of iron (Fe concentration determination) in whole blood of the dystrophic animal models. We performed blood analyses from three different strains with Muscular Dystrophy (Dmdmdx/ J, SJL/J and A/J) and from healthy (C57BL/6J) mice strain adopted as the control group. The results emphasize that iron levels in whole blood male samples of SJL/J and A/J are significantly higher. Related to the gender for Dmdmdx/J and SJL/J mouse strains the comparison emphasizes that iron level increase for males. The study of Fe behavior in whole blood mice samples with muscular dystrophy contributes to its diagnosis, providing a better understanding of this disease.Resumo IPEN-doc 23901 Iron determination in whole blood samples of dystrophic mice strains using X-ray fluorescence spectrometry2017 - SILVA, DALTON G.N. da; METAIRON, SABRINA; ZAMBONI, CIBELE B.; SUZUKI, MIRIAM F.; BUENO JUNIOR, CARLOS R.; RIZZUTTO, MARCIA de A.The use of alternative analytical techniques to investigate specific electrolytes in body fluids has increased in past years with the goal of adding advantages and simplifications, compared to the procedures used in conventional clinical practice. Specifically, in this study, a portable X-ray spectrometer (PXRFS) from Amptek (model X-123 SDD) with Ag X-ray mini-tube has been employed for the whole blood iron measurements in mice species with Muscular Dystrophy (Dmdmdx/J, SJL/J and A/J). The term whole blood refers to solid (cells) and liquid (plasma) components in blood. Usually the conventional procedures for clinical tests are performed in serum using samples of 0.5 to 1.0 mL. However, when the biological material is scarce (case of small size animal model, i.e. mice), the possibility to use whole blood became a fascinating alternative for clinical practice: the Energy Dispersive X-Ray Fluorescence (EDFRX) analyses, using PXRFS, can be done using 50 μL (the total body blood in mice is around 1.0 mL). In this study, we investigated whole blood samples of dystrophic mice strains, a genetic disease that cannot be reversed. In this investigation, twenty samples were investigated. The blood samples were obtained from Jackson Laboratory (Maine, USA) and further inbred at IPEN. The whole blood was collected by the retro–orbital venous plexus and immediately transferred to filter paper (Whatman n41), prior to its coagulation. No anticoagulants or reagents have been used to prevent interference on the results. This procedure simplify the preparation and conservation of biological samples: it is not a destructive procedure and the sample does not need refrigeration. The collection was performing according to the rules approved by Animal Research Ethics Committee (087/99). A comparative study between EDXFX and INAA (Instrumental Neutron Activation Analysis) data was also carried out and the results are in good agreement. This alternative procedure was capable, in a few minutes, to determine whole blood Fe concentrations higher than 6.5 mgL-1, without chemical digestion using direct and non-destructive analysis. The study of Fe behavior in whole blood samples of mice with muscular dystrophy contributes to its diagnosis.Artigo IPEN-doc 22423 Determination of iron content in whole blood in different mouse strains using a portable XRFS spectrometer2016 - ZAMBONI, CIBELE B.; METAIRON, SABRINA; SUZUKI, MIRIAM F.; BAHOVSCHI, VANESSA; RIZZUTTO, MARCIA A.Artigo IPEN-doc 22408 Inorganic elements in blood of mice immunized with snake venom using NAA and XRF techniques2016 - METAIRON, S.; ZAMBONI, C.B.; SUZUKI, M.F.; SILVA, L.F.F.L. da; RIZZUTTO, M.A.Artigo IPEN-doc 21759 Comparative study of inorganic elements determined in whole blood from Dmdsup(mdx)/J mice strain by EDXRF and NAA analytical techniques2016 - REDIGOLO, M.M.; SATO, I.M.; METAIRON, S.; ZAMBONI, C.B.Resumo IPEN-doc 21552 Inorganic elements in blood of mice (HIII) immunized with snake venom using INAA and EDXRF techniques2015 - METAIRON, SABRINA; ZAMBONI, CIBELE; SUZUKI, MIRIAM; SILVA, LAIS L. da; RIZZUTTO, MARCIAArtigo IPEN-doc 21226 Differences in iron concentration in whole blood of animal models using NAA2014 - BAHOVSCHI, V.; ZAMBONI, C.B.; LOPES SILVA, L.F.F.; METAIRON, S.; MEDEIROS, I.M.M.A.
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