RODRIGO CRESPO MOSCA
21 resultados
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Resumo IPEN-doc 25946 Smart dental fillings with ruthenium nanoparticles-enhanced photobiomodulation therapy for pulp-dentin regeneration2019 - YOUNG, N.C.; MOSCA, R.C.; ZEITUNI, C.A.; ARANY, P.R.Resumo IPEN-doc 24853 Efficacy of photobiomodulation therapy in mitigating skin radiation damage2018 - MOSCA, RODRIGO C.; SANTOS, SOFIA N.; NOGUEIRA, GESSE E.C.; PEREIRA, DAISA L.; COSTA, FRANCIELLI C.; ARANY, PRAVEEN; ZEITUNI, CARLOS A.Background: The use of sophisticated radiation dose delivery and fractionation has significantly improved cancer care. One of these involves localized, sustained ionizing dose delivery termed brachytherapy. Despite it therapeutic efficacy, specific side effects of brachytherapy include localized skin damage and breakdown for which only palliative treatments are currently available. The use of low dose biophotonics treatments to promote tissue healing is termed photobiomodulation (PBM) therapy. The aim of this study was to evaluate efficacy and molecular pathways of PBM therapy using two common wavelengths, red and near-infrared (NIR) to treat radiation wounds in athymic mice subjected to brachytherapy (sustained ionizing radiation from 125I seeds). Study Design/Materials and Method: A pilot study was performed with thirty-six athymic mice were accomplished for 60 days and divided into six groups: Surgical Control Group (No radiation and no PBM treatments); Radiation Control Group (125I seed 0.4252 mCi, no PBM); NIR-PBM Control Group (NIR PBM alone, LED at l¼880 nm); Red-PBM Control Group (Red PBM alone LED at l¼660 nm); Radiation- NIR PBM Group; Radiation-Red PBM Group. Following 21 days, radiation-induced wounds are evident. PBM treatments (both wavelengths with output power 40mW for 20 s, fluence 20 J/cm2 on top of implantation site) were performed every week up to 60 days. Wounds were evaluated every 7 days digital imaging, Laser Doppler Flowmetry (LDF) and tissue temperature with a thermographic camera. We also performed mPET-CT imaging using radioactive fluorodeoxyglucose (18F-FDG) at 51 and 81 days post-implantation. Animals were sacrifices progressively at each time point to correlate clinical observations with imaging and molecular tissue analyses. Tissues were collected to analyze molecular pathways correlating with inflammation, immune response, wound healing and angiogenesis using mRNA (qRT-PCR) and protein expression (immunostaining). Results: Both PBM treated groups demonstrated significant (p<0.05) improvements in skin radiation wound healing as compared to radiation group. Distinct improvements in clinical wound size and closure, improved tissue perfusion and reduced inflammation as evidenced by decreased wound thermal images. These wounds were also noted to have significant differences in the cytokine profiles (TGF-b, VEGF and PDGF) correlating with better healing responses. Radiation damage reduces brown fat composition that can potentially contribute to additional radiation-associated morbidities. The mPET-CT imaging noted significant preservation of brown fat composition in PBM-treated radiation alone groups. Further validation of these pathways is ongoing. Conclusion: Within the parameters of this study, PBM treatments demonstrated improved healing in radiation wounds due to ionizing radiation from 125I seeds. Ongoing work is examining the precise molecular pathways contributing to these therapeutic benefits. It is hoped this study will enable further development of this innovative therapy for managing side- effects from radiation treatments.Artigo IPEN-doc 24820 Learning from clinical phenotypes2018 - RAHMAN, S.U.; MOSCA, R.C.; REDDY, S.G.; NUNEZ, S.C.; ANDREANA, S.; MANG, T.S.; ARANY, P.R.This narrative review on the use of biophotonics therapies for management of oral diseases is written as a tribute to Prof. Crispian Scully. His seminal contributions to the field are highlighted by the detailed, comprehensive description of clinical presentations of oral diseases. This has enabled a more thorough, fundamental understanding of many of these pathologies by research from his group as well as inspired mechanistic investigations in many groups globally. In the same vein, a major emphasis of this narrative review is to focus on the evidence from human case reports rather than in vitro or in vivo animal studies that showcases the growing and broad impact of biophotonics therapies. The similarities and differences between two distinct forms of low-dose biophotonics treatments namely photodynamic therapy and photobiomodulation therapy are discussed. As evident in this review, a majority of these reports provide promising evidence for their clinical efficacy. However, a lack of adequate technical details, precise biological rationale, and limited outcome measures limits the current utility of these treatments. Future investigations should attempt to address these shortcomings and develop better designed, rigorous, controlled studies to fully harness the tremendous potential of low-dose biophotonics therapies.Artigo IPEN-doc 24762 [Ru(bipy)3]2+ nanoparticle-incorporate dental light cure resin to promote photobiomodulation therapy for enhanced vital pulp tissue repair2018 - MOSCA, RODRIGO C.; YOUNG, NICHOLAS; ZEITUNI, CARLOS A.; ARANY, PRAVEEN R.The use of nanoparticle on dental light cure resin is not new, currently several compounds (nanoadditives) are used to promote better communication between the restorative material and biological tissues. The interest for this application is growing up to enhance mechanical proprieties to dental tissue cells regeneration. Bioactive nanoparticles and complex compounds with multiple functions are the major target for optimizing the restorative materials. In this work, we incorporate [Ru(bipy)3]2+ nanoparticles, that absorbs energy at 450 nm (blue-light) and emits strongly at ~620 nm (red-light), in PLGA Microspheres and insert it in Dental Light Cure Resin to promote the Photobiomodulation Therapy (PBM) effects to accelerate dental pulp repair by in vitro using cytotoxicity and proliferation assay.Resumo IPEN-doc 23458 Brazil radioactive sources production for cancer treatment2016 - ROSTELATO, MARIA E.; SOUZA, CARLA D.; ZEITUNI, CARLOS A.; MOURA, JOAO A.; MARQUES, JOSE R.O.; COSTA, OSVALDO L.; FEHER, ANSELMO; RODRIGUES, BRUNA T.; SOUZA, DAIANE C.B. de; PELEIAS JUNIOR, FERNANDO S.; SORGATTI, ANDERSON; MOSCA, RODRIGO; MOURA, EDUARDO S. de; ABREU, RODRIGO T.; SOUZA, RAQUEL V. DE; NOGUEIRA, BEATRIZ R.The modality, known as brachytherapy, was performed in Brazil by only a hand full of hospitals at an extremely high cost. For producing new sources, five major areas must be considered: 1) source production: nuclear activation and/or radiochemical reaction; 2) welding; 3) Quality control: leakage tests; 4) Dosimetry and metrology; 5) Operational procedures; 6) validation studies. To perform all steps, a multidisciplinary team works together to overcome difficulties. - Iridium-192 pellets: In Brazil there are 140 machines with pellets that replacement every 5 years. Our new production line has assembly, welding and quality control hot cells. - Iridium-192 wires: Produced since 1999. The wire is activated at IPENs IEA-R1 reactor for 30 hours with 5x1013 n/cm-2.s-1 neutron flux resulting in 192 mCi maximum activity. - Iridium-192 seed: New seed for ophthalmic cancer treatment. The irradiation device presented 90% activity homogeneity. We are still testing in-vivo. - Iodine-125 seeds: Largely used in low dose brachytherapy. I-125 binding yield achieved with our new reaction was 80%; Laser welding presented 70% efficiency. Approved in all leakage tests. - Other ongoing projects: Veterinary brachytherapy, Waste management, Radionecrosis healing with laser, calibrations sources production, linear accelerator calculations for hospitals, sources with polymeric matrix Our Iodine-125 seeds will be available in 2018. All other projects are advancing. We will continue to develop new products hoping to help the Brazilian population fight against cancer. For producing new sources, five major areas must be considered: 1) source production: nuclear activation and/or radiochemical reaction; 2) welding; 3) Quality control:eakage tests; 4) Dosimetry and metrology; 5) Operational procedures; 6) validation studies. To perform all steps, a multidisciplinary team works together to overcome difficultiesResumo IPEN-doc 22982 Using AlGaInP laser in cutaneous radionecrosis healing induced by 125I seed in an athymic murine model2016 - MOSCA, R.C.; ZEITUNI, C.A.; LIMA, L.J.; COSTA, F.C.; RIBEIRO, M.S.Resumo IPEN-doc 22981 Low-level laser therapy to treat cutaneous radionecrosis induced by 125I seed in a murine model2016 - MOSCA, RODRIGO C.; RIBEIRO, MARTHA S.; SOUZA, CARLA D. de; ZEITUNI, CARLOS A.Artigo IPEN-doc 22676 Odontoameloblastoma: Report of two cases2009 - MOSCA, RODRIGO C.; MARQUES, MARCIA M.; BARBOSA, SANDRA C.; MARCUCCI, MARCELO; OLIVEIRA, JEFFERSON X.; LASCALA, CESAR A.Artigo IPEN-doc 22582 Cystic hygroma: characterization by computerized tomography2008 - MOSCA, RODRIGO C.; PEREIRA, GISELE A.; MANTESSO, A.Resumo IPEN-doc 20676 Tissue-engineered skin reconstructed using adipose tissue-derived stem cells and karatinocytes on irradiated human dermis from tissue bank2014 - MATHOR, M.B.; MOSCA, R.C.; STEFFENS, D.; PELISSARI, C.; MANTESSO, A.
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