KALAN BASTOS VIOLIN
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Artigo IPEN-doc 27734 The effect of Ipomoea carnea on maternal reproductive outcomes and fetal and postnatal development in rats2021 - GOTARDO, ANDRE T.; LIPPI, LUCIANA L.; VIOLIN, KALAN B.; BEVILACQUA, ESTELA M.A.F.; GORNIAK, SILVANA L.Ipomoea carnea is a toxic plant found in Brazil and other tropical countries. The plant contains the alkaloids calystegines and swainsonine, which inhibit key cellular enzymes and cause systematic cell death. It is known that swainsonine is excreted in the amniotic fluid of dams exposed to the plant. Thus, the aim of this study was to verify whether the toxic effect of I. carnea on fetuses is due to exclusively the passage of the active principle of the plant through the placenta, or if the placentotoxic effect of swainsonine could collaborate in the adverse effects observed in the fetus. The teratogenic effects of exposure to the toxic principles of I. carnea were evaluated not only using the conventional protocol but also at later stages in the postnatal developmental period. Females were treated, from gestation day (GD) 6 until GD19, with 0.0, 1.0, 3.0 or 7.0 g/kg body weight of I. carnea dry leaves. The plant did not induce changes in reproductive performance or biochemical profile of the dams. Dams that received the highest dose of I. carnea showed cytoplasmic vacuolization in the liver, kidney and placental tissue. I. carnea promoted different lectin binding patterns in different areas of placental tissue. No fetal skeletal or visceral malformations was observed. The postnatal evaluation revealed a lower litter weight and a lower pup body weight one day after birth in the group that received the highest dose of I. carnea. Physical milestones were unaffected by the treatments. Female pups from all experimental groups exhibited a delay in achieving a negative geotaxis response. The results show that the toxic principle of I. carnea produces injury in utero in mothers and fetuses, but these deleterious effects were better demonstrated using postnatal evaluation.Resumo IPEN-doc 25500 Alumina porous microspheres produced by snowballing technique, a new approach towards developing a suitable ceramic column for Tc-99m generators and other applications2018 - VIOLIN, K.B.; GOIA, T.S.; BRESSIANI, A.H.A.; BRESSIANI, J.Tecnetium-99m generators requires an alumina column to adsorb and hold Molybdenum-99m, this alumina needs to have an increased specific surface area but if the particles are too small they can clog the glass column filter and impair the functioning of it. Considering manufacturing particles of spherical shape, micro-scale size of less than 1mm and loose arrangement still presents a challenge, despite the existence of many processes to obtain such structures involving several steps they lack pores. Microspheres are of great interest because they have a high packing capability, if combined with pores the possibility of possessing an increased surface area leads them to a wide range of applications. The microarchitecture represented by microporosity gives an adsorption potential to microspheres. Snowballing technique is a new process shown to be a suitable method for obtaining ceramic spheres without additives or binders. The method developed is based on a mechanical continuous movement of the particles, relying on the normal ability of the ceramic powders to aggregate themselves while rolling in a cylindrical container for long periods. The physical forces involved in the process, gravity, particle attraction, centripetal force and shocking transforms the ceramic aggregates into rounds with an asteroid like shape on its surface [1]. With this method it was possible obtain microspheres with 40% of porosity and rough surface measuring between 1mm to less than 250μm, according to the sieves used to separate them. This work presents the obtaining of alumina microspheres through Snowballing technique for use on Tc-99m generators. In an effort to broaden the range of application, architecture and microstructures are major aspects of concern hence the obtained microspheres can be also used for other purposes.Resumo IPEN-doc 21321 Effects of ipomoea carnea in pla-cental tissue. Evaluation in rodents and ruminants2015 - GOTARDO, A.T.; LIPPI, L.L.; VIOLIN, K.B.; GORNIAK, S.L.Artigo IPEN-doc 22558 Improved osseointegration of long-term stored SLA implant by hydrothermal sterilization2016 - SHI, XINGLING; XU, LINGLI; VIOLIN, KALAN B.; LU, SHENGTese IPEN-doc 21200 Implantes cerâmicos e metálicos - caracterização da osteointegração por imuno-histoquímica, lectina-histoquímica e marcadores fluorescentes2015 - VIOLIN, KALAN B.Biomateriais, cerâmicos ou metálicos, para emprego em tecido ósseo, são importantes ferramentas na medicina regenerativa com intuito de reparar, restaurar, restituir, sustentar, tratar e substituir tecido lesionado. A análise da resposta biológica aos biomateriais é um grande desafio, considerando os aspectos inerentes do tecido ósseo mineralizado e as características físicas dos biomateriais. A compreensão do comportamento celular envolvido nesse conjunto tem o intuito de desenvolver biomateriais mais aptos e adequados ao tecido ósseo o qual ele é destinado. O objetivo deste estudo foi avaliar a osteointegração de implantes cerâmicos e metálicos após ensaio in vivo, por meio de técnicas histoquímicas com colorações de Giemsa-Eosina (GE), Hematoxilina-Eosina (HE), Tricrômico de Masson-Goldner (MG), Tricrômico de Masson (TM), Tricrômico de Gomori (GO) e Picrossírus (PS), Azul de Toluidina (AT) além de técnicas imunohistoquímica (IHQ), lectina-histoquímica (LHQ) e marcadores fluorescentes. Implantes cerâmicos à base de fosfatos de cálcio: hidroxiapatita (HAp), β-fosfato tricálcico (β-TCP) e mistura dos dois (BCP) 1:1 em peso, foram produzidos por gelcasting de espuma e pela inovadora técnica da bola de neve. Os implantes à base de titânio comercialmente puro (Ticp) e da Liga Ti-13Nb-13Zr (Liga) foram produzidos por metalurgia do pó; sendo macroporosos, com a adição de diferentes polímeros naturais, ou microporosos, com elevadas densidades, submetidos a diferentes tratamentos de superfície. A avaliação da osteointegração foi realizada em tíbia de coelhos após períodos de reparação, em conjuntos osso-implante: não-descalcificados e descalcificados. Na análise histológica por microscopia óptica com colorações histoquímicas, os conjuntos apresentaram crescimento ósseo com osteointegração em todos os implantes. A análise por fluorescência mostrou as fases e áreas marcadas de acordo com o período de aplicação do marcador, indicando intenso remodelamento ósseo em todo o osso, o crescimento ósseo no interior dos poros e ao redor dos implantes para os marcadores tetraciclina e calceína. Com a análise de IHQ foram avaliados os marcadores para osteopontina (OP), osteonectina (ON) e osteocalcina (OC), e com LHQ foram avaliadas 5 lectinas. O melhor marcador por IHQ foi para OP, havendo correlação entre a marcação com WGA pela LHQ e OP pela IHQ. A técnica de LHQ foi bem sucedida em criar perfis de comparação entre os biomateriais. A maior marcação por RCA-1 para todos os implantes cerâmicos e metálicos, indicam que esta é um promissor marcador para diferenciação em estudos de osteointegração, bem como a marcação por sWGA e PNA nos implantes de Liga.Artigo IPEN-doc 20684 Manufacturing of porous ceramic spheres using calcium phosphates by a mechanical method without additives or binders2014 - VIOLIN, KALAN B.; GOIA, TAMIYE S.; ISHIKAWA, KUNIO; BRESSIANI, JOSE C.; BRESSIANI, ANA H. de A.Resumo IPEN-doc 20598 Microspheres of BCP produced by snowballing technique for multipurpose application2014 - VIOLIN, K.B.; GOIA, T.S.; ISHIKAWA, K.; BRESSIANI, J.C.; BRESSIANI, A.H.A.Artigo IPEN-doc 20597 Manufacturing of porous ceramic spheres using calcium phosphates by a mechanical method without additives or binders2014 - VIOLIN, KALAN B.; GOIA, TAMIYE S.; ISHIKAWA, KUNIO; BRESSIANI, JOSE C.; BRESSIANI, ANA H. de A.Resumo IPEN-doc 20503 Avaliação histológica de implantes cerâmicos macroporosos de diferentes composições2014 - RIBEIRO, CHRISTIANE; VIOLIN, K.B.; BRESSIANI, A.H.A.; BRESSIANI, J.C.Artigo IPEN-doc 20400 Biocompatibilidade de cerâmicas macroporosas: uma avaliação qualitativa da osteointegração2014 - RIBEIRO, C.; ARAUJO, O.M.M.; VIOLIN, K.B.; BRESSIANI, A.H.A.
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