ANA PAULA FUNARI
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Dissertação IPEN-doc 25669 Avaliação morfológica e biomecânica de tecido tendinoso humano esterilizado por radiação ionizante2017 - FUNARI, ANA P.O crescente aumento do interesse no desenvolvimento em técnicas cirúrgicas menos invasivas, como nas reconstruções tendíneas e ligamentares, tem levado ao aumento das pesquisas referentes ao uso de aloenxertos esterilizados por radiação ionizante. O processamento por radiação ionizante é um método seguro e não deixa resíduos, sendo utilizado como esterilização final. O presente estudo teve como proposta avaliar os efeitos da aplicação de radiação ionizante, produzida por fonte de 60Co, em amostras de tendões humanos pré processados de doadores multiorgãos obtidas por meio de colaboração com Bancos de Tecidos. O pré-processamento das amostras deu-se por métodos químicos e preservação por congelamento em -80 °C. As doses aplicadas no processamento por radiação foram de 12,5 kGy, 15,0 kGy e 25,0 kGy, cada uma com seu respectivo controle não irradiado. As amostras foram avaliadas por meio de testes histológicos, ópticos e biomecânicos, com o objetivo de analisar possíveis modificações morfológicas e estruturais. Os resultados apresentados demonstraram que o processamento por ultrassom e peróxido de hidrogênio causaram alterações na morfologia dos tecidos, o que ocasionou danos à sua estrutura, inviabilizando as amostras. Nas amostras processadas por álcool e antibiótico não foram observados danos na rede de colágeno pela aplicação da radiação. Os resultados dos testes biomecânicos apresentaram diferenças significativas entre os métodos aplicados. As amostras processadas com álcool e antibiótico apresentaram perda pouco significativa no módulo de elasticidade, comparadas às amostras processadas por ultrassom e peróxido de hidrogênio que mantiveram a propriedade viscoelástica. Contudo na dose de 12,5 kGy foi observado um aumento no módulo elástico e na viscoelasticidade. Com base nas análises, podemos concluir que o método de processamento com álcool, antibiótico e irradiação demonstrou menor dano, tanto na biomecânica quanto na esterilização, sendo que as amostras irradiadas a 15,0 e 25,0 kGy apresentaram características semelhantes ao controle não irradiado.Resumo IPEN-doc 25540 Optical and histological evaluation of human tendon tissue sterilized by ionizing radiation2018 - FUNARI, ANA P.; ANTEBI, URI; SANTOS, LUIZ A.; VIEIRA, DANIEL P.; MIRANDA, JURANDIR T. de; ALVES, NELSON M.; FREITAS, ANDERSON Z. de; MATHOR, MONICA B.Sterilization by irradiation is a technique that is used by tissue banks aiming to eliminate contamination of human allografts, being a safe method, free of residue and used as final sterilization. After the tissue procurement, these undergo a series of processing stages and then are packaged and preserved by freezing. Despite aseptic care of the material those may be subjected to sterilization in the final packing by ionizing radiation, raising the security level of sterility of the tissue. The aim of this study was to evaluate the effects of application of ionizing radiation, produced by 60Co source in human tendons preprocessed (A-alcohol + antibiotic; B- H2O2 + ultrasound) obtained through collaboration with tissue banks and preserved by freezing in -80° C, the radiation absorbed doses in processing were 12.5, 15 and 25 kGy, each one with their corresponding non-irradiated control, to examine possible structural or morphological alterations. The irradiated samples and their controls were analyzed by means of optical coherence tomography (OCT) and optical coherence tomography polarization sensitive (PS-OCT), and histological tests had been stained with hematoxylin-eosin (HE). According to the results the tissue processed with alcohol/antibiotic in conjunction with irradiation proved to be the most effective.Artigo IPEN-doc 24768 Radiolabeled GX1 peptide for tumor angiogenesis imaging2018 - OLIVEIRA, ERICA A. de; FAINTUCH, BLUMA L.; SEO, DANIELE; BARBEZAN, ANGELICA B.; FUNARI, ANA; TARGINO, ROSELAINE C.; MORO, ANA M.Early and accurate detection of primary or metastatic tumors is of great value in staging, treatment management, and prognosis. Tumor angiogenesis plays an essential role in the growth, invasion, and metastatic spread of solid cancers, and so, is a promising approach for tumor imaging. The GX1 (CGNSNPKSC) peptide was identified by phage display library and has been investigated as a marker for human cancers. This study aims to evaluate the 99mTc-HYNIC-PEG4-c (GX1) as a biomarker for tumor imaging. Our results showed that GX1 specifically binds to tumor cells in vitro. SKMEL28 and MDA-MB231 cells achieved total binding peak at 60 min of incubation. For B16F10 and MKN45 cells, the total and specific binding were similar during all time points, while A549 cell line showed rapid cellular total uptake of the tracer at 30 min of incubation. Biodistribution showed low non-specific uptakes and rapid renal excretion. Melanoma tumors showed enhanced GX1 uptake in animal model at 60 min, and it was significantly blocked by cold peptide. The radiotracer showed tumor specificity, especially in melanomas that are highly vascularized tumors. In this sense, it should be considered in future studies, aiming to evaluate degree of angiogenesis, progression, and invasion of tumors.Artigo IPEN-doc 24102 Optical and histological evaluation in human tendon tissue sterilized by ionizing radiation2017 - FUNARI, ANA P.; ANTEBI, URI; SANTOS, LUIZ A.; VIEIRA, DANIEL P.; MIRANDA, JURANDIR T. de; ALVES, NELSON M.; FREITAS, ANDERSON Z. de; MATHOR, MONICA B.Sterilization by irradiation is a technique that is used by tissue banks aiming to eliminate contamination of human allografts, being a safe method, free of residue and used as final sterilization. After the tissue procurement, these undergo a series of processing stages and then are packaged and preserved by freezing. Despite aseptic care of the material those may be subjected to sterilization in the final packing by ionizing radiation, raising the security level of sterility of the tissue. The aim of this study was to evaluate the effects of application of ionizing radiation, produced by 60Co source in human tendons preprocessed (A-alcohol + antibiotic; B- H2O2 + ultrasound) obtained through collaboration with tissue banks and preserved by freezing in -80° C, the radiation absorbed doses in processing were 12.5, 15 and 25 kGy, each one with their corresponding non-irradiated control, to examine possible structural or morphological alterations. The irradiated samples and their controls were analyzed by means of optical coherence tomography (OCT) and optical coherence tomography polarization sensitive (PS-OCT), and histological tests had been stained with hematoxylin-eosin (HE). According to the results the tissue processed with alcohol/antibiotic in conjunction with irradiation proved to be the most effective.Artigo IPEN-doc 21158 Qualitative and semi quantitative analysis in the healing area of athymic nude mice skin engrafted with human skin sterilized with gamma radiation2015 - MIRANDA, JURANDIR T. de; BRINGEL, FABIANA; ALVES, NELSON M.; ANTEBI, URI; FUNARI, ANA P.; MATHOR, MONICA B.Artigo IPEN-doc 21124 Dosimetry of an animal irradiation system2015 - ALVES, NELSON M.; FUNARI, ANA P.; MIRANDA, JURANDIR T.; NAPOLITANO, CELIA M.; GONCALVES, JOSEMARY A.C.; BUENO, CARMEN C.