CLAUDIA REGINA CECCHI
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Artigo IPEN-doc 24222 Efficient non-invasive plasmid-DNA administration into tibialis cranialis muscle of “little” mice2017 - CECCHI, C.R.; HIGUTI, E.; LIMA, E.R.; VIEIRA, D.P.; SQUAIR, P.L.; PERONI, C.N.; BARTOLINI, P.Background: An alternative treatment for growth hormone deficiency based on hGH-DNA administration, followed by electro gene transfer, was investigated by injecting the plasmid into surgically exposed or non-exposed quadriceps or tibialis muscle of immunodeficient “little” mice. Methods: An optimization of electrotransfer conditions via a new combination of high/low voltage pulses is presented. After 3 days, serum hGH was determined and in a 28-day assay, the relative growth parameters were compared. Results: Both groups exhibited similar results: 5.0 ± 2.2 (SD) and 3.5 ± 0.9 ng hGH/ml (P>0.05; n=7) for the exposed quadriceps and non-exposed tibialis treatments, respectively. The final body weight increases were 16.1% for the quadriceps and 18.9% for the tibialis group. The tail and nose-to-tail length increases were 4.5% and 7.1% for the quadriceps and 4.8 and 4.6% for the tibialis group. The right and left femur length increases, obtained from radiographic measurements, were 16.9% and 12.7% for the quadriceps and 19.4% and 12.3% for the tibialis, respectively. A non-significant difference between exposed quadriceps and non-exposed tibialis treatments (P=0.48) was confirmed via a completely integrated statistical analysis. Circulating mIGF-1 levels were 126 ± 47, 106 ± 93 (P>0.05) and 38 ± 15 ng/ml for the quadriceps, tibialis and saline treatments, respectively. Conclusion: These results show that hGH-DNA administration into non-exposed tibialis muscle followed by the new HV/LV electrotransfer protocol was an equally efficient, less traumatic treatment,Capítulo IPEN-doc 21588 Modelos de terapia gênica baseados na expressão de hormônio de crescimento em células humanas e na administração de DNA plasmidial em camundongos anões2015 - PERONI, CIBELE N.; CECCHI, CLAUDIA R.; HIGUTI, ELIZA; BARTOLINI, PAOLOResumo IPEN-doc 21241 Early treatment for growth hormone deficiency based on naked DNA administration in dwart mice allows efficient catch-up growth2015 - HIGUTI, ELIZA; CECCHI, CLAUDIA R.; LIMA, ELIANA R.; AAGAARD, LARS; JENSEN, THOMAS G.; BARTOLINI, PAOLO; PERONI, CIBELE N.Artigo IPEN-doc 20061 A novel homologous model for gene therapy of swarfism by non-viral transfer of the mouse growth hormone gene into immunocompetent dwarf mice2014 - CECCHI, CLAUDIA R.; HIGUTI, ELIZA; OLIVEIRA, NELIO A.J.; LIMA, ELIANA R.; JAKOBSEN, MARIA; DAGNAES-HANSEN, FREDERIK; BARTOLINI, PAOLO; PERONI, CIBELE N.Artigo IPEN-doc 18345 Growth responses following a single intra-muscular hGH plasmid administration compared to daily injections of hGH in dwarf mice2012 - HIGUTI, ELIZA; CECCHI, CLAUDIA R.; OLIVEIRA, NELIO A.J.; VIEIRA, DANIEL P.; JENSEN, THOMAS G.; JORGE, ALEXANDER A.L.; BARTOLINI, PAOLO; PERONI, CIBELE N.Artigo IPEN-doc 15564 Long-term human growth hormone expression and partial phenotypic correction by plasmid-base gene therapy in an animal model of isolated growth hormone deficiency2010 - OLIVEIRA, NELIO A.J.; CECCHI, CLAUDIA R.; HIGUTI, ELIZA; OLIVEIRA, JOAO E.; JENSEN, THOMAS G.; BARTOLINI, PAOLO; PERONI, CIBELE N.Artigo IPEN-doc 13178 Secretion of mouse growth hormone by transduced primary human keratinocytes: prospects for an animal model of cutaneous gene therapy2008 - PERONI, CIBELE N.; CECCHI, CLAUDIA R.; ROSAURO, CRISTIANE W.; NONOGAKI, SUELY; BOCCARDO, ENRIQUE; BARTOLINI, PAOLO