DÉCIO DOS SANTOS PINTO JUNIOR

DÉCIO DOS SANTOS PINTO JUNIOR

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The security in utilizing in vitro reconstituted human oral epithelium. An oncogenetic pathway study
2009 - KLEIGBEIL, M.; MATHOR, M.B.; GIUDICE, F.G.; YOSHITO, D.; PINTO JUNIOR, D.S.
Effect of topical 5-ALA mediated photodynamic therapy on proliferation index of keratinocytes in 4-NQo-induced potentially malignant oral lesions
2013 - BARCESSAT, ANA R.; HUANG, ISAAC; ROSIN, FLAVIA P.; PINTO JUNIOR, DECIO dos S.; ZEZELL, DENISE M.
Fractionation can improve photodynamic therapy (PDT) efficacy for potentially malignant oral lesion treatment. The aim of this study was to demonstrate the apoptosis/proliferation index of oral keratinocytes after two sessions of topical 5-ALA-mediated PDT in 4-Nitroquinoline-1-oxide-induced potentially malignant oral lesion, and to suggest the ideal interval between PDT sessions. Immuno-histochemical tests for proliferating cell nuclear antigen and caspase-3, and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay were performed at 6 h, 24 h, 48 h, and 72 h time intervals after PDT. The number of positive cells showing caspase-3 expression was significantly higher, mainly at 6 h after PDT. In the first cycle of PDT, the highest frequency of positive cells for TUNEL was found at 24 h. At 72 h after PDT, proliferating cell nuclear antigen positive cells increased significantly, indicating that there was an epithelial response in direction towards DNA repair and cell proliferation at this time. Because cell proliferation increases and cell death index decreases at 72 h after PDT, it is recommended that the interval between the PDT sessions must not be longer than 2 days up to total lesion remission.
Comparison of two cellular harvesting methods for primary human oral culture of keratinocytes
2009 - KLINGBEIL, MARIA F.G.; HERSON, MARISA R.; CRISTO, ELIER B.; PINTO JUNIOR, DECIO dos S.; YOSHITO, DANIELE; MATHOR, MONICA B.
Evaluation of microRNA expression in head and neck squamous cell carcinoma cell lines and in primary culture of oral keratinocytes
2011 - ANDREGHETTO, FLAVIA M.; KLINGBEIL, MARIA F.G.; SOARES, RENATA M.; SITNIK, ROBERTA; PINTO JUNIOR, DECIO dos S.; MATHOR, MONICA B.; NUNES, FABIO D.; SEVERINO, PATRICIA
Is it safe to utilize in vitro reconstituted human oral epithelium? An oncogenetic pathway study
2012 - KLINGBEIL, MARIA F.G.; MATHOR, MONICA B.; GIUDICE, FERNANDA S.; YOSHITO, DANIELE; PINTO JUNIOR, DECIO dos S.
Cytotoxic effects of mistletoe (Viscum album L.) in head and neck squamous cell carcinoma cell lines
2013 - KLINGBEIL, MARIA F.G.; XAVIER, FLAVIA C.A.; SARDINHA, LUIZ R.; SEVERINO, PATRICIA; MATHOR, MONICA B.; RODRIGUES, RODRIGO V.; PINTO JUNIOR, DECIO S.
Head and neck squamous cell carcinoma is a complex disease with several etiologic factors and different molecular changes that may trigger certain events; it is also globally one of the most common malignancies in this topography. Extracts from Viscum album L. (VA) (mistletoe) have been used as adjuvant therapies with promising results in several types of cancer, mainly in European countries. In vitro studies have demonstrated that various types of VA may have cytotoxicity in carcinoma cells, activating the apoptotic cascade or leading cells to necrosis. This study aimed to verify the effects of three types of VA extracts (Iscador Qu Spezial, Iscador P and Iscador M) in squamous cell carcinoma of the tongue cell lines SCC9 and SCC25, not previously studied. A concentration of 0.3 mg/ml (IC50) of the drugs induced apoptosis, affecting gene expression and protein levels of AKT, PTEN and CYCLIN D1. It was concluded that VA extracts have a cytotoxic effect on SCC9 and SCC25 cell lines, but while SCC9 cell line was more resistant to the action of the drugs, Iscador Qu Spezial and Iscador M have higher cytotoxic potential in both cell lines compared to Iscador P.