JOSE GILMAR FRANCO
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Artigo IPEN-doc 27891 Glycine max oil physical-chemical quality obtained of irradiated seeds2021 - FRANCO, J.G.; SILVA, R.M.G. da; SUGUIMOTO, R.Y.; FRANCO, S.S.H.; VILLAVICENCIO, A.L.C.H.; ARTHUR, V.Ionizing radiation applied to agriculture has mainly benefited food production, as it reduces natural losses caused by physiological processes, as well as eliminating or reducing microorganisms, parasites and pests. In addition, this technique also allows the production of mutants with characteristics of greater productivity, precocity, smaller size, greater resistance to diseases and pests. These mutants are used to obtain new varieties of species of agronomic interest. The objective of this study was to evaluate the physic-chemical quality of the oil extracted from seeds of G. max (soybean) obtained from irradiated seeds with different doses of gamma radiation (0, 25, 50, 75 and 100 Gy of a Cobalt-60 source, type Gammacell-220 installed in CENA-USP). The physic-chemical analyzes included: AOCS Cd-3d-63, AOCS Cd-3-25 and AOCS Cd-1-25, Acids Index (I.A.), Saponification Index (I.S.), Iodide Index (I.I.), respectively. All analytical determinations were performed at least in triplicates. The values found for I.A., for I.S. and for I.I., did not differ from the oil sample obtained from soybean seeds from control subjects (without irradiation) regardless of the concentration and dose of radiation applied to the seeds. The oil analyzed did not show physical-chemical variation in comparison with the oil obtained from non-irradiated seeds, thus suggesting the absence of modifications in these parameters after the genetic improvement induced by the radiation.Artigo IPEN-doc 27890 Propagation of mango seedlings of the cultivar "Tommy" by grafts irradiated2021 - FRANCO, S.S.H.; FRANCO, J.G.; FERRARI, L.; LEANDRO, R.S.R.; ARTHUR, P.B.; ARTHUR, V.Due to difficulties of vegetative propagating of any pants, many tests were performed with ionizing radiation aiming the modification of the characteristics of the plants for obtaining of the improved genotypes and of smaller size. Rootstock “Espada” cultivar, were used to obtain mango seedlings of “Tommy” cultivar. The cuttings were irradiated with different gamma radiation doses: 0 (control), 2.5, 5.0, 7.5 and 10.0 Gy, a dose rate of 0.323 kGy/h, in a source of Cobalt-60 type Gammacell-220, installed in the Center for Nuclear Energy in Agriculture, CENA-USP. After irradiation, was performed the grafting type cleft graft. It was made the first evaluation of height of the plants from the budding grafting after 24 months. Data were subjected by statistic program (SAS) and the means were compared by Tukey test (p <0.05). From the results obtained can be concluded that the doses of 2.5 Gy stimulated growth of the plants and the lethal dose was 10.0 Gy because don’t have development of plants.Artigo IPEN-doc 26263 Glycine max oil physical-chemical quality obtained of irradiated seeds2019 - FRANCO, JOSE G.; SILVA, REGILDO M.G. da; SUGUIMOTO, RODRIGO Y.; VILLAVICENCIO, ANNA L.C.H.; ARTHUR, VALTERIonizing radiation applied to agriculture has mainly benefited food production, as it reduces natural losses caused by physiological processes, as well as eliminating or reducing microorganisms, parasites and pests. In addition, this technique also allows the production of mutants with characteristics of greater productivity, precocity, smaller size, greater resistance to diseases and pests. These mutants are used to obtain new varieties of species of agronomic interest. The objective of this study was to evaluate the physic-chemical quality of the oil extracted from seeds of G. max (soybean) obtained from irradiated seeds with different doses of gamma radiation (0, 25, 50, 75 and 100 Gy of a Cobalt-60 source, type Gammacell-220 installed in CENA-USP). The physic-chemical analyzes included: AOCS Cd-3d-63, AOCS Cd-3-25 and AOCS Cd-1-25, Acids Index (I.A), Saponification Index (IS), Iodide Index (II), respectively. All analytical determinations were performed at least in triplicates. The values found for I.A., for I.S. and for I.I., did not differ from the oil sample obtained from soybean seeds from control subjects (without irradiation) regardless of the concentration and dose of radiation applied to the seeds. The oil analyzed did not show physical-chemical variation in comparison with the oil obtained from non-irradiated seeds, thus suggesting the absence of modifications in these parameters after the genetic improvement induced by the radiation.Artigo IPEN-doc 26262 Propagation of mango seedlings of the cultivar “Tommy” by grafts irradiated2019 - FRANCO, SUELY S.H.; FRANCO, JOSE G.; FERRARI, LUIZ; MACHI, ANDRE R.; LEANDRO, RODRIGO S.R.; ARTHUR, PAULA B.; ARTHUR, VALTERDue to difficulties of vegetative propagating of any pants, many tests were performed with ionizing radiation aiming the modification of the characteristics of the plants for obtaining of the improved genotypes and of smaller size. Rootstock “Espada” cultivar, were used to obtain mango seedlings of “Tommy” cultivar. The cuttings were irradiated with different doses of gamma radiation: 0 (control), 2.5, 5.0, 7.5 and 10.0 Gy, in a source of Cobalt-60 type Gammacell-220, installed in the Center for Nuclear Energy in Agriculture, CENA-USP. After irradiation, was performed the grafting type cleft graft. Was made the first evaluation of height of the plants from the budding grafting after 24 months. Data were subjected by statistic program (SAS) and the means were compared by Tukey test (p <0.05). From the results obtained can be concluded that the doses of 2.5 Gy stimulated growth of the plants and the lethal dose was 10.0 Gy because don’t have development of plants.Artigo IPEN-doc 25720 Efeitos da radiação gama na cor e características fisico-quimicas de abobrinha Cucurbita moschata minimamente processada2018 - SILVA, LUCIA C.A.S.; PIRES, JULIANA A.; ARTHUR, PAULA B.; ROSSI, RODRIGO S.; HARDER, MARCIA N.C.; FRANCO, MARIA A.H.; FRANCO, LUISA H.; FRANCO, JOSE G.; FRANCO, SUELY S.H.; FRANCO, CAMILO F. de O.; CAZE FILHO, JORGE; ARTHUR, VALTERArtigo IPEN-doc 25595 Low doses of Gamma Radiation in Soybean2018 - FRANCO, JOSÉ G.; FRANCO, SUELY S.H.; FRANCO, CAIO H.; FRANCO, LUISA H.; FRANCO, MARIA A.H.; ARTHUR, PAULA B.; VILLAVICENCIO, ANNA L.C.; ARTHUR, VALTERThe degree of radio sensitivity depends mainly on the species, the stage of the embryo in the irradiation, the doses used and the criteria used to measure the effect. One of the most common criteria for evaluating seed radio sensitivity is to measure the plant's average yield. The dried soybean seeds were exposed to low doses of gamma radiation from Cobalt-60, Gammacell-220 type source, at a dose rate of 0.245 kGy. In the first stage the objective was to study the effects of radiation stimulation on germination, growth and plant production. Four doses of radiation were applied as follows: 0 (control); 25; 50; 75 and 100 Gy. Seed germination harvested seed number and total yield were evaluated to identify the occurrence of stimulation. The low doses of gamma radiation in the seeds that stimulate the production were doses of 25, 50 and 75 Gy. In the second stage the objective was also to study the effects of radiation stimulation on germination, growth and plant production. In this case, a treatment with three doses of radiation was applied as follows: 0 (control); 12.5; 25.0 and 50.0 Gy. Seed germination harvested seed number and total yield were evaluated to identify the occurrence of stimulation. The number of soybean seeds and plants were handled following the standard seed production in Brazil. There is evidence in the results showing that the use of low doses of gamma radiation can stimulate the germination and the production of plants.Tese IPEN-doc 24981 Influência de diferentes doses de radiação gama (Cobalto60) em sementes de soja Glycine max (L.) visando aumento de produtividade2018 - FRANCO, JOSÉ G.A cultura da soja possui enorme importância econômica mundial e particularmente para o Brasil. Existem várias pesquisas voltadas para o aumento de produtividade e qualidade desta cultura, como melhoramento e alterações genéticas de cultivares. Vários trabalhos vêm demonstrando que a irradiação ionizante pode aumentar a produção de plantas, surgindo assim como um método alternativo para aumentar a produção de culturas de importância econômica. O objetivo deste estudo foi testar várias doses de irradiação ionizante, variando desde 10 Gy até 150 Gy, visando observar qual delas poderia desenvolver características benéficas, como aumento de produtividade e da qualidade do óleo. O trabalho foi desenvolvido durante três anos, sendo parte dele no campo e outra parte em casa de vegetação. Também foi avaliado o potencial genotóxico e a qualidade do óleo dos grãos produzido por indivíduos provenientes destas sementes irradiadas. No campo observou-se vários parâmetros com relação a planta, sendo que nenhum destes parâmetros apresentou diferenças significativas conforme o teste estatístico aplicado. Já com relação ao aumento de produtividade podemos concluir que houve diferença significativa entre os valores de produtividade da testemunha e das parcelas irradiadas com dose de 25 Gy, com aumento de 97,97%. Em ambiente protegido foram avaliados os efeitos de estimulação da radiação sobre a germinação e produção, sendo que os resultados indicaram que os tratamentos com doses de 12,5; 25; 50 e 75 Gy apresentaram diferença estatística significativa quando comparado a outros tratamentos, mas entre si os valores foram semelhantes. Na determinação do potencial genotóxico, avaliado pelo método do teste com Allium cepa, as amostras de óleo de soja provenientes de sementes irradiadas não apresentaram atividade genotóxico e citotóxica. Também pode-se concluir que o óleo obtido de sementes de indivíduos provenientes de sementes irradiadas, não apresentam diferença em comparação ao óleo obtido de sementes de indivíduos não irradiadas, onde foi possível comparar os valores dos índices referentes a acidez, saponificação, refração e índice de iodo, além da comprovação também de que estas doses positivas geram produtos limpos e livres de características depreciativas.Artigo IPEN-doc 24160 Low doses of gamma radiation in soybean2017 - FRANCO, JOSE G.; FRANCO, SUELY S.H.; FRANCO, CAIO H.; ARTHUR, VALTER; ARTHUR, PAULA B.; VILLAVICENCIO, ANNA L.C.The degree of radiosensitivity depends mostly on the species, the stage of the embryo at irradiation, the doses employed and the criteria used to measure the effect. One of the most common criteria to evaluate radiosensitivity in seeds is to measure the average plant production. Dry soya seeds were exposed to low doses of gamma radiation from source of Cobalt-60, type Gammecell-220, at 0.210 kGy dose rate. In order to study stimulation effects of radiation on germination, plant growth and production. A treatment with four radiation doses was applied as follows: 0 (control); 12.5; 25.0 and 50.0 Gy. Seed germination and harvested of number of seeds and total production were assessed to identify occurrence of stimulation. Soya seeds number and plants were handled as for usual seed production in Brazil. The low doses of gamma radiation in the seeds that stimulate the production were the doses of 12.5 and 50.0 Gy. The results show that the use of low doses of gamma radiation can stimulate germination and plant production.Artigo IPEN-doc 24149 Quarantine treatment by gamma radiation for different development stages of Callosobruchus maculatus in bean Vigna sinensis2017 - ARTHUR, VALTER; FONTES, LUCIA da S.; ARTHUR, PAULA B.; MACHI, ANDRE R.; HARDER, MARCIA N.C.; ROSSI, RODRIGO S.; FRANCO, JOSE G.; FRANCO, SUELY S.H.The loss of stored grain caused by insects generates a problem of economic order of importance, due to concern about the increased supply of food for the world population is expanding. Associated with this fact, there is the problem of nutritional deficiency due to lack of protein, especially for the less privileged populations. The use of ionizing radiation in grains and products stored without a doubt can solve the problem of the losses in these products, since it does not induce resistance to insects and leaves no toxic residue in the products, and is considered an effective and safe method. The aim of the experiment was to determine the effect of ionizing radiation from cobalt-60 as a quarantine treatment for the different stages of development of Callosobruchus maculatus (Fabr., 1972) (Coleóptera, Chysomilidae) in bean Vigna sinensis. The experiment was conducted in the laboratory of Radiobiology and Environment CENA / USP., Piracicaba, SP, Brazil. Bean samples infested with eggs, larvae, pre-pupae and pupae C. maculatus, the experiment consisted of 4 replicates for each stage of the insect's life cycle, and each repetition consisted of 20 individuals (eggs, larvae, pre-pupae and pupae), a total of 200 subjects per treatment which were irradiated with doses of 0 (control), 25, 50, 75 and 100 Gy, a source of cobalt-60, Gammabeam-650 type, in a rate dose of 1.3 kGy / h. The experiment was conducted in a room with a relative of 25 ± 2 ° C temperature and humidity of 70 ± 5%. After 35 days of irradiation process were carried out evaluations of the number of insects emerged in each repetition within the treatments. From the results obtained it was concluded that the dose lethal to eggs and larvae was 25 Gy, while for pre-pupae was 50 Gy, to pupae 100 Gy was not sufficient to control the adult emergence.Artigo IPEN-doc 21094 Gamma radiation in the control of insects in animal feed2015 - ARTHUR, PAULA B.; ARTHUR, VALTER; SILVA, LUCIA C.A.S.; FRANCO, SUELY S.H.; FRANCO, JOSE G.; HARDER, MARCIA N.C.; VILLAVICENCIO, ANNA L.C.H.