VIVIANE PEREIRA GOULART
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Artigo IPEN-doc 21297 Noninvasive monitoring of photodynamic therapy on skin neoplastic lesions using the optical attenuation coefficient measured by optical coherence tomography2015 - GOULART, VIVIANE P.; SANTOS, MOISES O. dos; LATRIVE, ANNE; FREITAS, ANDERSON Z.; CORREA, LUCIANA; ZEZELL, DENISE M.Photodynamic therapy (PDT) has become a promising alternative for treatment of skin lesions such as squamous cell carcinoma. We propose a method to monitor the effects of PDT in a noninvasive way by using the optical attenuation coefficient (OAC) calculated from optical coherence tomography (OCT) images. We conducted a study on mice with chemically induced neoplastic lesions and performed PDT on these lesions using homemade photosensitizers. The response of neoplastic lesions to therapy was monitored using, at the same time, macroscopic clinical visualization, histopathological analysis, OCT imaging, and OCT-based attenuation coefficient measurement. Results with all four modalities demonstrated a positive response to treatment. The attenuation coefficient was found to be 1.4 higher in skin lesions than in healthy tissue and it decreased after therapy. This study shows that the OAC is a potential tool to noninvasively assess the evolution of skin neoplastic lesions with time after treatment.Resumo IPEN-doc 20905 Biochemical changes in normal skin caused by squamous cell carcinoma using FTIR spectroscopy2015 - LIMA, CASSIO; GOULART, VIVIANE; CORREA, LUCIANA; ZEZELL, DENISEArtigo IPEN-doc 20857 Biochemical changes in cutaneous squamous cell carcinoma submitted to PDT using ATR-FTIR spectroscopy2015 - LIMA, CASSIO A.; GOULART, VIVIANE P.; CASTRO, PEDRO A.A. de; CORREA, LUCIANA; BENETTI, CAROLINA; ZEZELL, DENISE M.Nonmelanoma skin cancers are the most common form of malignancy in humans. Between the traditional treatment ways, the photodynamic therapy (PDT) is a promising alternative which is minimally invasive and do not requires surgical intervention or exposure to ionizing radiation. The understanding of the cascade of effects playing role in PDT is not fully understood, so that define and understand the biochemical events caused by photodynamic effect will hopefully result in designing better PDT protocols. In this study we investigated the potential of the FTIR spectroscopy to assess the biochemical changes caused by photodynamic therapy after 10 and 20 days of treatment using 5-aminolevulinic acid (ALA) as precursor of the photosensitizer photoporphyrin IX (PpIX). The amplitude values of second derivative from vibrational modes obtained with FTIR spectroscopy showed similar behavior with the morphological features observed in histopathological analysis, which showed active lesions even 20 days after PDT. Thus, the technique has the potential to be used to complement the investigation of the main biochemical changes that photodynamic therapy promotes in tissue.Artigo IPEN-doc 20832 ATR-FTIR spectroscopy for the assessment of biochemical changes in skin due to cutaneous squamous cell carcinoma2015 - LIMA, CASSIO A.; GOULART, VIVIANE P.; CORREA, LUCIANA; PEREIRA, THIAGO M.; ZEZELL, DENISE M.Nonmelanoma skin cancers represent 95% of cutaneous neoplasms. Among them, squamous cell carcinoma (SCC) is the more aggressive form and shows a pattern of possible metastatic profile. In this work, we used Fourier transform infrared spectroscopy (FTIR) spectroscopy to assess the biochemical changes in normal skin caused by squamous cell carcinoma induced by multi-stage chemical carcinogenesis in mice. Changes in the absorption intensities and shifts were observed in the vibrational modes associated to proteins, indicating changes in secondary conformation in the neoplastic tissue. Hierarchical cluster analysis was performed to evaluate the potential of the technique to differentiate the spectra of neoplastic and normal skin tissue, so that the accuracy obtained for this classification was 86.4%. In this sense, attenuated total reflection (ATR)-FTIR spectroscopy provides a useful tool to complement histopathological analysis in the clinical routine for the diagnosis of cutaneous squamous cell carcinoma.Resumo IPEN-doc 20643 Non-invasive monitoring of PDT on skin squamous cell carcinoma using the optical attenuation coefficient measured by OCT2014 - GOULART, VIVIANE P.; SANTOS, MOISES O. dos; CORREA, LUCIANA; LATRIVE, ANNE; LIMA, CASSIO A. de; ZEZELL, DENISE M.