VIVIANE PEREIRA GOULART
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Artigo IPEN-doc 22385 Using fourier transform infrared spectroscopy to evaluate biological effects induced by photodynamic therapy2016 - LIMA, CASSIO A.; GOULART, VIVIANE P.; CORREA, LUCIANA; ZEZELL, DENISE M.Background and Objective: Vibrational spectroscopic methods associated with multivariate statistical techniques have been succeeded in discriminating skin lesions from normal tissues. However, there is no study exploring the potential of these techniques to assess the alterations promoted by photodynamic effect in tissue. The present study aims to demonstrate the ability of Fourier Transform Infrared (FTIR) spectroscopy on Attenuated total reflection (ATR) sampling mode associated with principal component-linear discriminant analysis (PC-LDA) to evaluate the biochemical changes caused by photodynamic therapy (PDT) in skin neoplastic tissue. Materials and Methods: Cutaneous neoplastic lesions, precursors of squamous cell carcinoma (SCC), were chemically induced in Swiss mice and submitted to a single session of 5-aminolevulinic acid (ALA)-mediated PDT. Tissue sections with 5mm thickness were obtained from formalin-fixed paraffin-embedded (FFPE) and processed prior to the histopathological analysis and spectroscopic measurements. Spectra were collected in mid-infrared region using a FTIR spectrometer on ATR sampling mode. Principal Component-Linear Discriminant Analysis (PC-LDA) was applied on preprocessed second derivatives spectra. Biochemical changes were assessed using PCA-loadings and accuracy of classification was obtained from PC-LDA . Results: Sub-bands of Amide I (1,624 and 1,650 cm1 ) and Amide II (1,517 cm1 ) indicated a protein overexpression in non-treated and post-PDT neoplastic tissue compared with healthy skin, as well as a decrease in collagen fibers (1,204, 1,236, 1,282, and 1,338 cm1 ) and glycogen (1,028, 1,082, and 1,151 cm1 ) content. Photosensitized neoplastic tissue revealed shifted peak position and decreased b-sheet secondary structure of proteins (1,624 cm1 ) amount in comparison to non-treated neoplastic lesions. PC-LDA score plots discriminated non-treated neoplastic skin spectra from post-PDT cutaneous lesions with accuracy of 92.8%, whereas non-treated neoplastic skin was discriminated from healthy tissue with 93.5% accuracy and post-PDT cutaneous lesions was discriminated from healthy tissue with 89.7% accuracy. Conclusion: PC-LDA was able to discriminate ATR-FTIR spectra of non-treated and post-PDT neoplastic lesions, as well as from healthy skin. Thus, the method can be used for early diagnosis of premalignant skin lesions, as well as to evaluate the response to photodynamic treatment.Artigo IPEN-doc 22306 Evaluation of squamous cell skin carcinoma using ATR-FTIR spectroscopy associated to cluster analysis2014 - LIMA, C.A.; GOULART, V.; ZEZELL, D.M.Cluster Analysis were used as an unsupervised classification technique to differentiate FTIR spectra of normal and tumor skin. The results shown satisfactory separation in samples analyzed, highlighting the potential of the technique for diagnostic purposes.Resumo IPEN-doc 21566 Aplicação de técnicas quimiométricas associadas a espectroscopia FT-IR para discriminar tecido sadio de lesões cutâneas potenciamente malignas2015 - LIMA, CASSIO A.; GOULART, VIVIANE P.; ZEZELL, DENISE M.Resumo IPEN-doc 20905 Biochemical changes in normal skin caused by squamous cell carcinoma using FTIR spectroscopy2015 - LIMA, CASSIO; GOULART, VIVIANE; CORREA, LUCIANA; ZEZELL, DENISEArtigo IPEN-doc 20857 Biochemical changes in cutaneous squamous cell carcinoma submitted to PDT using ATR-FTIR spectroscopy2015 - LIMA, CASSIO A.; GOULART, VIVIANE P.; CASTRO, PEDRO A.A. de; CORREA, LUCIANA; BENETTI, CAROLINA; ZEZELL, DENISE M.Nonmelanoma skin cancers are the most common form of malignancy in humans. Between the traditional treatment ways, the photodynamic therapy (PDT) is a promising alternative which is minimally invasive and do not requires surgical intervention or exposure to ionizing radiation. The understanding of the cascade of effects playing role in PDT is not fully understood, so that define and understand the biochemical events caused by photodynamic effect will hopefully result in designing better PDT protocols. In this study we investigated the potential of the FTIR spectroscopy to assess the biochemical changes caused by photodynamic therapy after 10 and 20 days of treatment using 5-aminolevulinic acid (ALA) as precursor of the photosensitizer photoporphyrin IX (PpIX). The amplitude values of second derivative from vibrational modes obtained with FTIR spectroscopy showed similar behavior with the morphological features observed in histopathological analysis, which showed active lesions even 20 days after PDT. Thus, the technique has the potential to be used to complement the investigation of the main biochemical changes that photodynamic therapy promotes in tissue.Artigo IPEN-doc 20832 ATR-FTIR spectroscopy for the assessment of biochemical changes in skin due to cutaneous squamous cell carcinoma2015 - LIMA, CASSIO A.; GOULART, VIVIANE P.; CORREA, LUCIANA; PEREIRA, THIAGO M.; ZEZELL, DENISE M.Nonmelanoma skin cancers represent 95% of cutaneous neoplasms. Among them, squamous cell carcinoma (SCC) is the more aggressive form and shows a pattern of possible metastatic profile. In this work, we used Fourier transform infrared spectroscopy (FTIR) spectroscopy to assess the biochemical changes in normal skin caused by squamous cell carcinoma induced by multi-stage chemical carcinogenesis in mice. Changes in the absorption intensities and shifts were observed in the vibrational modes associated to proteins, indicating changes in secondary conformation in the neoplastic tissue. Hierarchical cluster analysis was performed to evaluate the potential of the technique to differentiate the spectra of neoplastic and normal skin tissue, so that the accuracy obtained for this classification was 86.4%. In this sense, attenuated total reflection (ATR)-FTIR spectroscopy provides a useful tool to complement histopathological analysis in the clinical routine for the diagnosis of cutaneous squamous cell carcinoma.Resumo IPEN-doc 14281 Efeito do laser de Nd:YAG na resistencia a desmineralizacao do esmalte quando associado a aplicacao de fluoreto2009 - GOULART, VIVIANE P.; ZEZELL, DENISE M.Resumo IPEN-doc 17465 Mechanisms of high intensity lasers for caries prevention and erosion2011 - ANA, P.A.; BACHMANN, L.; BENETTI, C.; GOULART, V.P.; ZEZELL, D.M.Resumo IPEN-doc 16035 FTIR spectroscopy monitoring chemical changes in enamel dentin and bone due to laser irradiation2010 - ZEZELL, DENISE M.; ANA, PATRICIA A.; BENETTI, CAROLINA; QUINTO JUNIOR, JOSE; PEREIRA, THIAGO M.; GOULART, VIVIANEResumo IPEN-doc 15314 Characterization by ATR-FTIR of irradiated bone2009 - BENETTI, C.; SANTOS, M.O.; GOULART, V.P.; ZEZELL, D.M.