Analysis of the combined effects of resveratrol and radiation on lung cancer cells
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2017
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Integrative Cancer Biology & Research
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Mucoepidermoid lung carcinoma is frequently manifested by
obstructive trachea symptoms. It is necessary to develop effective
curative or palliative therapeutic strategies for treating mucoepidermoid
lung carcinoma through administration of compounds that improve
ionizing radiation treatment, thereby increasing the effects of the
treatment on tumor cells while minimizing the effects on normal tissues
surrounding the tumor cells. Resveratrol is a polyphenolic phytoalexin
present in wines and several plants and has a broad spectrum of
biological activities, including antioxidant and anticarcinogenic effects.
The biological effects of ionizing radiation plus resveratrol have been
examined in different types of cell in many studies. Here, we aimed to
verify the effects of resveratrol on mucoepidermoid lung carcinoma
cells NCI-H292 exposed to ionizing radiation. In vitro studies in
NCI-H292 cell culture using neutral red uptake assays showed that the
cytotoxicity index (IC50) of resveratrol was 401.5μM, and the lethal
dose 50 % (LD50) of ionizing radiation in the absence of resveratrol
was 693 Gy. In vitro micronucleus assays were then performed to
verify the genotoxic effects of resveratrol, and fluorescence assays
were performed to evaluate the effects of resveratrol on the cell cycle,
repair and injury processes, cellular necrosis, and apoptosis. The results
demonstrated that 30μM resveratrol promote injury on NCI-H292 cells
after 24 h of irradiation. Therefore, this study provides results able
to support in vitro future investigations about radiosensitive potential
of resveratrol in lung cancer cells. There is a need to investigate
compounds with potential to improve the local control of lung cancer
promoted by radiation therapy. This way avoiding injuries to healthy
cells.
Como referenciar
MORENO, CAROLINA S.; ROGERO, SIZUE O.; GALISTEO JUNIOR, ANDRES J.; VIEIRA, DANIEL P.; CRUZ, AUREA S.; SAKURABA, ROBERTO K.; WELTMAN, EDUARDO; ROGERO, JOSE R. Analysis of the combined effects of resveratrol and radiation on lung cancer cells. Integrative Cancer Biology & Research, v. 1, n. 1, 2017. Disponível em: http://repositorio.ipen.br/handle/123456789/31151. Acesso em: 12 May 2024.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.