Analysis of the combined effects of resveratrol and radiation on lung cancer cells

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dc.contributor.authorMORENO, CAROLINA S.pt_BR
dc.contributor.authorROGERO, SIZUE O.pt_BR
dc.contributor.authorGALISTEO JUNIOR, ANDRES J.pt_BR
dc.contributor.authorVIEIRA, DANIEL P.pt_BR
dc.contributor.authorCRUZ, AUREA S.pt_BR
dc.contributor.authorSAKURABA, ROBERTO K.pt_BR
dc.contributor.authorWELTMAN, EDUARDOpt_BR
dc.contributor.authorROGERO, JOSE R.pt_BR
dc.coverageInternacionalpt_BR
dc.date.accessioned2020-04-29T19:10:27Z
dc.date.available2020-04-29T19:10:27Z
dc.date.issued2017pt_BR
dc.description.abstractMucoepidermoid lung carcinoma is frequently manifested by obstructive trachea symptoms. It is necessary to develop effective curative or palliative therapeutic strategies for treating mucoepidermoid lung carcinoma through administration of compounds that improve ionizing radiation treatment, thereby increasing the effects of the treatment on tumor cells while minimizing the effects on normal tissues surrounding the tumor cells. Resveratrol is a polyphenolic phytoalexin present in wines and several plants and has a broad spectrum of biological activities, including antioxidant and anticarcinogenic effects. The biological effects of ionizing radiation plus resveratrol have been examined in different types of cell in many studies. Here, we aimed to verify the effects of resveratrol on mucoepidermoid lung carcinoma cells NCI-H292 exposed to ionizing radiation. In vitro studies in NCI-H292 cell culture using neutral red uptake assays showed that the cytotoxicity index (IC50) of resveratrol was 401.5μM, and the lethal dose 50 % (LD50) of ionizing radiation in the absence of resveratrol was 693 Gy. In vitro micronucleus assays were then performed to verify the genotoxic effects of resveratrol, and fluorescence assays were performed to evaluate the effects of resveratrol on the cell cycle, repair and injury processes, cellular necrosis, and apoptosis. The results demonstrated that 30μM resveratrol promote injury on NCI-H292 cells after 24 h of irradiation. Therefore, this study provides results able to support in vitro future investigations about radiosensitive potential of resveratrol in lung cancer cells. There is a need to investigate compounds with potential to improve the local control of lung cancer promoted by radiation therapy. This way avoiding injuries to healthy cells.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipIDFAPESP: 13/03791-9pt_BR
dc.identifier.citationMORENO, CAROLINA S.; ROGERO, SIZUE O.; GALISTEO JUNIOR, ANDRES J.; VIEIRA, DANIEL P.; CRUZ, AUREA S.; SAKURABA, ROBERTO K.; WELTMAN, EDUARDO; ROGERO, JOSE R. Analysis of the combined effects of resveratrol and radiation on lung cancer cells. <b>Integrative Cancer Biology & Research</b>, v. 1, n. 1, 2017. Disponível em: http://repositorio.ipen.br/handle/123456789/31151.
dc.identifier.fasciculo1pt_BR
dc.identifier.orcid0000-0002-2517-7772pt_BR
dc.identifier.orcid0000-0002-0007-534Xpt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-2517-7772
dc.identifier.orcidhttps://orcid.org/0000-0002-0007-534X
dc.identifier.orcidhttps://orcid.org/0000-0003-3396-6277
dc.identifier.percentilfiSem Percentilpt_BR
dc.identifier.urihttp://repositorio.ipen.br/handle/123456789/31151
dc.identifier.vol1pt_BR
dc.relation.ispartofIntegrative Cancer Biology & Researchpt_BR
dc.rightsopenAccesspt_BR
dc.subjectdna damages
dc.subjectin vitro
dc.subjectionizing radiations
dc.subjectlungs
dc.subjectlymphokines
dc.subjectneoplasms
dc.subjectpolyphenols
dc.subjectradiation effects
dc.subjectradiosensitivity
dc.titleAnalysis of the combined effects of resveratrol and radiation on lung cancer cellspt_BR
dc.typeArtigo de periódicopt_BR
dspace.entity.typePublication
ipen.autorJOSE ROBERTO ROGERO
ipen.autorDANIEL PEREZ VIEIRA
ipen.autorSIZUE OTA ROGERO
ipen.autorCAROLINA DOS SANTOS MORENO
ipen.codigoautor1118
ipen.codigoautor3158
ipen.codigoautor1266
ipen.codigoautor6784
ipen.contributor.ipenauthorJOSE ROBERTO ROGERO
ipen.contributor.ipenauthorDANIEL PEREZ VIEIRA
ipen.contributor.ipenauthorSIZUE OTA ROGERO
ipen.contributor.ipenauthorCAROLINA DOS SANTOS MORENO
ipen.date.recebimento20-04
ipen.identifier.fiSem F.I.pt_BR
ipen.identifier.ipendoc26929pt_BR
ipen.type.genreArtigo
relation.isAuthorOfPublication7fa01758-0891-4c3a-a4de-6b2451991fc7
relation.isAuthorOfPublicationc2352608-be9c-4a73-be8c-571f10bb53d2
relation.isAuthorOfPublicationadecaf83-1d61-4fa2-b7e6-4cf91494610d
relation.isAuthorOfPublication56ce67f9-1538-4987-bb02-c00ad4b5ef8a
relation.isAuthorOfPublication.latestForDiscovery56ce67f9-1538-4987-bb02-c00ad4b5ef8a
sigepi.autor.atividadeROGERO, JOSE R.:1118:-1:Npt_BR
sigepi.autor.atividadeVIEIRA, DANIEL P.:3158:810:Npt_BR
sigepi.autor.atividadeROGERO, SIZUE O.:1266:-1:Npt_BR
sigepi.autor.atividadeMORENO, CAROLINA S.:6784:-1:Spt_BR
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