Effect of photodynamic antimicrobial chemotherapy on Candida albicans in the presence of glucose
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2019
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Photodiagnosis and Photodynamic Therapy
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Background: Candida albicans is an opportunistic commensal microorganism, often associated with severe infections
in immunosuppressed individuals. C. albicans has hexose transporters that may favor the intracellular
accumulation of photosensitizer (PS). the aims of this study were to investigate the influence of glucose load on
photodynamic antimicrobial chemotherapy (PACT); and the role that membrane transport system plays on this
therapy in the presence of glucose.
Material and methods: Strains of C. albicans were selected: ATCC 10231, YEM 12, YEM 13, YEM 14 and YEM 15.
All strains were grown aerobically on Sabouraud agar and incubated at 30 °C for 24 h. The strains were treated
with and without glucose, and divided into Control (no treatment), LED light (660 nm, 166 mW/cm2),
Photosensitizer (100 μM methylene blue) and PACT at 1, 3 and 6 min of irradiation groups. The colony forming
units were counted and data submitted to statistical analysis (ANOVA) and Tukey's test. The concentration of
methylene blue (MB) outside the yeast was measured by fluorescence spectroscopy.
Results: PACT inactivate C. albicans and the presence of glucose did not affect the killing effect for most strains.
Only YEM12 was partially affected by its presence. Regarding efflux systems, ABC overexpressing strain showed
a protective effect on the yeast cells. We observed that yeast with overexpression of major facilitator superfamily
(MFS) membrane pore tended to accumulate more MB in its cytoplasm, whereas strains that overexpressed ABC
pumps (ATP-binding cassette transporters) tended to decrease MB uptake and survive the photodynamic challenge.
Conclusion: Presence of glucose showed a small effect on PACT . The accumulation of MB on yeast induces more
photodynamic inactivation; however, the photodynamic efficacy depends on the type and characteristics of the
microbial strain.
Como referenciar
OLIVEIRA-SILVA, TAMIRES de; ALVARENGA, LETICIA H.; LIMA-LEAL, CINTIA; GODOY-MIRANDA, BIANCA; CARRIBEIRO, PAMELA; SUZUKI, LUIS C.; RIBEIRO, MARTHA S.; KATO, ILKA T.; PAVANI, CHRISTIANE; PRATES, RENATO A. Effect of photodynamic antimicrobial chemotherapy on Candida albicans in the presence of glucose. Photodiagnosis and Photodynamic Therapy, v. 27, p. 54-58, 2019. DOI: 10.1016/j.pdpdt.2019.05.032. Disponível em: http://repositorio.ipen.br/handle/123456789/30063. Acesso em: 12 May 2024.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.