Identification of irradiated wheat by germination test, DNA comet assay and electron spin resonance
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2001
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INTERNATIONAL MEETING ON RADIATION PROCESSING, 12th
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In several countries, there has been an increase in the use of radiation for food processing thus improving the quality
and sanitary conditions, inhibiting pathogenic microorganisms, delaying the natural aging process and so extending
product lifetime. The need to develop analytical methods to detect these irradiated products is also increasing. The goal
of this research was to identify wheat irradiated using different radiation doses. Seeds were irradiated with a gamma
60Co source (Gammacell 220 GC) in the Centro de Energia Nuclear na Agricultura and the Instituto de Pesquisas
Energeticas e Nucleares. Dose rate used were 1.6 and 5.8 kGy/h. Applied doses were 0.0, 0.10, 0.25, 0.50, 0.75, 1.0, and !
2.0 kGy. After irradiation, seeds were analysed over a 6 month period. Three different detection methods were
employed to determine how irradiation had modified the samples. Screening methods consisted of a germination test
measuring the inhibition of shooting and rooting and analysis of DNA fragmentation. The method of electron spin
resonance spectroscopy allowed a better dosimetric evaluation. These techniques make the identification of irradiated
wheat with different doses possible. r 2002 Elsevier Science Ltd. All rights reserved.
Como referenciar
BARROS, A.C.; FREUND, M.T.L.; VILLAVICENCIO, A.L.C.H.; DELINCEE, H.; ARTHUR, V. Identification of irradiated wheat by germination test, DNA comet assay and electron spin resonance. In: SADAT, T. (ed.); EHLERMANN, D. (ed.); MILLER, A. (ed.). In: INTERNATIONAL MEETING ON RADIATION PROCESSING, 12th, Mar. 25-30, 2001, Avignon, France. Proceedings... p. 423-426. Disponível em: http://repositorio.ipen.br/handle/123456789/16364. Acesso em: 19 Feb 2025.
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