International collaborative study to establish the 2nd WHO International Standard of Human Recombinant Fillicle Stimulating Hormone for Bioassai
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2010
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REUNIAO ANUAL DA SOCIEDADE BRASILEIRA DE BIOQUIMICA E BIOLOGIA MOLECULAR, 39.
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Resumo
The aim of the International Collaborative Study proposed by the National
Institute for Biological Standards and Control (WHO) is to calibrate a new
preparation of human follicle stimulating hormone (hFSH) relative to the 1st
International Standard for bioassay of recombinant-hFSH (WHO 92/642), which is
almost exhausted and requires to be replaced. The candidate preparation and four
other samples, which correspond to this preparation stored at elevated
temperatures (K, L, M, N), were analyzed qualitatively and quantitatively in our
laboratory, by reversed-phase high-performance liquid chromatography (RPHPLC).
The candidate content determined in six assays (8.59 ± 0.41 μg/ampoule)
was 20% lower, when compared with the current standard. Based on this, an
average value of 110 IU FSH per ampoule could be assigned to this preparation.
The main isoform of the candidate preparation showed a lower retention time (22.5
± 0.83 min) relative to WHO 92/642 (22.8 ± 0.81 min) and also to an internal
reference preparation (22.6 ± 0.86 min). These differences, though, were not
significant (p>0.05). Practically identical contents were also determined for K, L, M
and N ampoules (8.44 ± 0.20; 8.56 ± 0.33; 8.57 ± 0.38; 8.69 ± 0.30 μg/ampoule,
respectively). No significant differences in FSH retention times between the
candidate preparation and the ampoules stored at elevated temperatures were
observed.
Como referenciar
OLIVEIRA, J.E.; ALMEIDA, B.E.; DAMIANI, R.; BARTOLINI, P.; RIBELA, M.T.C.P. International collaborative study to establish the 2nd WHO International Standard of Human Recombinant Fillicle Stimulating Hormone for Bioassai. In: REUNIAO ANUAL DA SOCIEDADE BRASILEIRA DE BIOQUIMICA E BIOLOGIA MOLECULAR, 39., 18-21 de maio, 2010, Foz do Iguacu, PR. Resumos... p. 71. Disponível em: http://repositorio.ipen.br/handle/123456789/21256. Acesso em: 13 Feb 2025.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.