Physical-chemical and biological characterization of different preparations of equine chorionic gonadotropin
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2016
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Journal of Veterinary Science
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Ovarian stimulation with commercial preparations of equine chorionic gonadotropin (eCG) produces extremely variable responses in
domestic animals, ranging from excessive stimulation to practically no stimulation, when applied on the basis of their declared unitage. This
study was conducted to analyze four commercial preparations from different manufacturers via reversed-phase HPLC (RP-HPLC) in
comparison with a reference preparation and an official International Standard from the World Health Organization. The peaks obtained by
this qualitative and quantitative physical–chemical analysis were compared using an in vivo bioassay based on the ovarian weight gain of
prepubertal female rats. The RP-HPLC data showed one or two peaks close to a main peak (tR = 27.9 min), which were related to the in vivo
bioactivity. Commercial preparations that have this altered peak showed very little or no in vivo activity, as demonstrated by rat ovarian weight
and in peripubertal gilts induced to ovulate. Overall, these findings indicate that RP-HPLC can be a rapid and reliable tool to reveal changes
in the physicochemical profile of commercial eCG that is apparently related to decreased biological activity of this hormone.
Como referenciar
ALVAREZ, RAFAEL H.; NATAL, FABIO L.N.; RIBELA, MARIA T.C.P.; ALMEIDA, BEATRIZ E. de; OLIVEIRA, JOAO E. de; BARTOLINI, PAOLO. Physical-chemical and biological characterization of different preparations of equine chorionic gonadotropin. Journal of Veterinary Science, v. 17, n. 4, p. 459-465, 2016. DOI: 10.4142/jvs.2016.17.4.459. Disponível em: http://repositorio.ipen.br/handle/123456789/27152. Acesso em: 13 Feb 2025.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.