Protein crosslinking by high energy irradiation - towards the development of size-controlled biocompatible nanocarriers
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2016
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CONGRESSO LATINO-AMERICANO DE ORGAOS ARTIFICIAIS E BIOMATERIAIS, 9; CONGRESSO DA SOCIEDADE LATINO AMERICANA DE BIOMATERIAIS, ORGAOS ARTIFICIAIS E ENGENHARIA DE TECIDOS, 13.
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Resumo
Protein and peptide based delivery systems are on the spotlight considering their unique
properties specially related to site specific delivery and high biocompatibility despite other
biopharmaceutical advantages. Following the recent success of the so-called NAB platform, most
of the attention has been driven towards the development of cosolvent -free or crosslinker-free
technologies capable of producing protein nanoparticles with a specific size or shape, in the seek
for better or preferential tumor uptake and low or negligible toxicity among other features. Within
this context irradiation stands a tool capable of promoting protein crosslinking and sterilization of
the systems simultaneously, in which combined or not with other techniques may allow the design
of nanocarriers without the need of monomers or toxic crosslinkers. This research details the use
of high energy irradiation towards the design of size-controlled protein-based nanocarriers for
drug delivery. The albumin or papain based nanocarriers were designed by combining
desolvation/solvation techniques followed by gamma irradiation or gamma irradiation alone. Sizecontrolled
nanocarriers were manufactured up to 80 nm, as determined by dynamic light
scattering, depending upon the protein or the presence of cosolvent. Nanocarrier morphology
was evaluated by transmission electron microscopy, and protein crosslinking was evaluated by
means of bityrosine formation using fluorescence measurements. Final applications of the
developed systems comprise relevant potential for the delivery of radiopharmaceuticals or
chemotherapic agents.
Como referenciar
VARCA, G.H.; MOHAMED, L.B.; FAZOLIN, G.N.; OLIVEIRA, J.P.; BATISTA, J.G.; LUGAO, A.B. Protein crosslinking by high energy irradiation - towards the development of size-controlled biocompatible nanocarriers. In: CONGRESSO LATINO-AMERICANO DE ORGAOS ARTIFICIAIS E BIOMATERIAIS, 9; CONGRESSO DA SOCIEDADE LATINO AMERICANA DE BIOMATERIAIS, ORGAOS ARTIFICIAIS E ENGENHARIA DE TECIDOS, 13., 24-27 de agosto, 2016, Foz do Iguaçu, PR. Abstract... p. 1162-1162. Disponível em: http://repositorio.ipen.br/handle/123456789/27474. Acesso em: 16 Apr 2024.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.