Comparative analyses of ion release, pH and multispecies biofilm formation between conventional and bioactive gutta-percha
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2016
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International Endodontic Journal
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AimTo analyse the effect of commercial and experimental gutta-percha with the addition of niobium phosphate glass on biofilm formation by oral bacteria from human dental plaque. Additional pH and elemental release of the materials were analysed.
MethodologyThe multispecies biofilm was grown anaerobically from plaque bacteria on standardized discs of each material: hydroxyapatite (HA), gutta-percha pellets (OBT) (Obtura pellets, Shoreline, CT, USA), ProTaper gutta-percha (PTP) (ProTaper Universal Gutta-Percha Points, Dentsply Maillefer, Ballaigues, Switzerland), EndoSequence BC gutta-percha (GBC) (Brasseler USA, Savannah, GA, USA), experimental gutta-percha associated with niobium phosphate glass (GNB) and niobium phosphate glass (NPG). Specimens (n=5 per group and per incubation period) were incubated in brain-heart infusion broth for 3, 14 and 30days, at 37 degrees C, and stained using live/dead viability assay. Images were analysed by confocal laser scanning microscopy (CLSM) and the total biovolume (mm(3)), viable bacteria biovolume (mm(3)), and live percentage (%) were quantified. For pH measurement, specimens of each material (n=3) were immersed in phosphate-buffered saline at 37 degrees C, and pH was monitored in multiple intervals, up to 30days. For elemental analysis, additional specimens (n=3) were immersed in deionized water and elemental release was analysed by ICP-OES (inductively coupled plasma - optical emission spectrometry) at time intervals of 3, 14 and 30days. Differences between groups were evaluated by the two-way analysis of variation (anova) with Tukey's post hoc test (P<0.05).
ResultsThe lowest total biovolume at 30days was found in GNB, GBC and NPG. GNB had the lowest viable bacteria biovolume (mean value) at 30days (P<0.05), and the lowest live percentage of bacteria at 3 and 30days (P<0.05), whilst NPG had the lowest live percentage at 14days (P<0.05). GNB had the highest pH (8.45) after 30days (P<0.05), and the greatest Zn and Na release at all time intervals (P<0.05). Both GBC and GNB had significantly higher Ca release at 14 and 30days.
ConclusionGNB and GBC reduced biofilm formation, GNB had the lowest amount of viable bacteria in biofilms with the highest pH, and high Zn and Na release values after 30days.
Como referenciar
CARVALHO, C.N.; WANG, Z.; SHEN, Y.; GAVINI, G.; MARTINELLI, J.R.; MANSO, A.; HAAPASALO, M. Comparative analyses of ion release, pH and multispecies biofilm formation between conventional and bioactive gutta-percha. International Endodontic Journal, v. 49, n. 11, p. 1048-1056, 2016. DOI: 10.1111/iej.12558. Disponível em: http://repositorio.ipen.br/handle/123456789/27846. Acesso em: 24 Apr 2024.
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