Incorporation of the methylene blue photosensitizer in the presence of glucose in strains of candida albicans with multidrug efflux systems
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2017
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Lasers in Surgery and Medicine
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Background: Infectious diseases are responsible for 60% of
deaths in developing countries.Candida albicans cause severe
infection in immunocompromised patients and the commonly
used treatments have not been effective against microbial
resistance. Photodynamic therapy (PDT) is a new promising
strategy to microbial inactivation based on the use of
photosensitizer (PS) in the presence of oxygen and activation
by light to form reactive oxygen species.C.albicanshas glucose
sensors which could facilitate the entrance of the PS. The
objective of this study was to evaluate the role of glucose in the
photodynamic inactivation of C. albicans.
Study: C. albicans ATCC 10231, YEM 12, YEM 13, YEM
14, and YEM15were aerobically grown on Sabouraud agar
and incubated at 308C for 24 hours. Microbial inoculants
were divided into 4 groups with and without glucose:
Control; Only irradiation; PS toxicity and PDT groups with
three irradiation times.After going throughthe treatments,
the colony-forming units were counted and the data were
subjected to statistical analysis (ANOVA) and Tukey test.
To measure the concentration ofMB, fluorescence spectroscopy
and flow cytometry were used.
Results: We observed that yeast with overexpression of
Major Facilitator Superfamily membrane pore tend to
accumulate more MB in its cytoplasm, whereas strains
that overexpress ABC pumps demonstrated the greater
survival against the photodynamic challenge.
Conclusion: PDT is an efficient strategy against C.
albicans and the presence of glucose can intervene in the
photodynamic effect. The presence of MB in the intracellular
region of the yeast predisposes to greater photodynamic
inactivation, however, its germicidal capacity is
related to the type and characteristics of the microbial.
Como referenciar
SILVA, TAMIRES; ALVARENGA, LETICIA; LEAL, CINTIA; MIRANDA, BIANCA G.; SUZUKI, LUIZ C.; KATO, ILKA; RIBEIRO, MARTHA; PAVANI, CHRISTIANE; PRATES, RENATO. Incorporation of the methylene blue photosensitizer in the presence of glucose in strains of candida albicans with multidrug efflux systems. Lasers in Surgery and Medicine, v. 49, n. 4, p. 438-438, 2017. Disponível em: http://repositorio.ipen.br/handle/123456789/27922. Acesso em: 15 Mar 2025.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.