Development of three-dimensional cellular culture system for testing of biological effects of radiations in tumoral and non-tumoral models
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2017
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CONGRESSO DA SOCIEDADE BRASILEIRA DE BIOCIENCIAS NUCLEARES, 12.
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Pre-clinical drug testing is currently based on based on monolayer or 2D (2D) cell cultures and,
despite the large-scale use of this form of culture, there is already scientific evidence that the cellular
disposition in monolayers does not adequately simulate tissue physiology, as it prevents cells from
expressing their characteristics in a manner analogous to that found in the organism. For this purpose,
the work aimed to produce three dimensional structures, referred as spheroids, using magnetic
levitation by adding iron nanoparticles to the cultures and with the aid of magnets. Electron
microscopy showed particles with about 20nm in diameter. FTIR (Fourier-transform infrared
spectroscopy) analysis showed stretches compatible with iron and amino acid (Lysine) binding. The
images showed the formation of spherical bodies until the ninth day. LnCap spheroid diameter varied
from (mean ± error) 434.407 ± 50.018 m (5th day) to 264.574 ± 13.184 μm (9th day). Cultures of
CHO ranged from 229.237 ± 5.278 to 236.719 ± 12.910 μm in the same period. Spheres generated
by magnetic levitation could be measured by digital means and compared throughout the experiment.
The tool can be used to test the biological effects of radiation and / or radiopharmaceuticals in culture.
Como referenciar
BONFIM, LETICIA; GONÇALVES, KARINA O.; VIEIRA, DANIEL P. Development of three-dimensional cellular culture system for testing of biological effects of radiations in tumoral and non-tumoral models. In: CONGRESSO DA SOCIEDADE BRASILEIRA DE BIOCIENCIAS NUCLEARES, 12., 09-11 de outubro, 2017, São Paulo, SP. Anais... Disponível em: http://repositorio.ipen.br/handle/123456789/28433. Acesso em: 12 May 2024.
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