MUC1 aptamer-capped mesoporous silica nanoparticles for controlled drug delivery and radio-imaging applications
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2017
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Nanomedicine: Nanotechnology, Biology, and Medicine
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Mucin 1 (MUC1) is a cell surface protein overexpressed in breast cancer. Mesoporous silica nanoparticles (MSNs) loaded with safranin
O, functionalized with aminopropyl groups and gated with the negatively charged MUC1 aptamer have been prepared (S1-apMUC1) for
specific targeting and cargo release in tumoral versus non-tumoral cells. Confocal microscopy studies showed that the S1-apMUC1
nanoparticles were internalized in MDA-MB-231 breast cancer cells that overexpress MUC1 receptor with subsequent pore opening and
cargo release. Interestingly, the MCF-10-A non-tumorigenic breast epithelial cell line that do not overexpress MUC1, showed reduced (S1-
apMUC1) internalization. Negligible internalization was also found for S1-ap nanoparticles that contained a scrambled DNA sequence as
gatekeeper. S2-apMUC1 nanoparticles (similar to S1-apMUC1 but loaded with doxorubicin) internalized in MDA-MB-231 cells and
induced a remarkable reduction in cell viability. Moreover, S1-apMUC1 nanoparticles radio-labeled with 99mTc (S1-apMUC1-Tc) showed
a remarkable tumor targeting in in vivo studies with MDA-MB-231 tumor-bearing Balb/c mice.
© 2017 Elsevier Inc. All rights reserved.
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PASCUAL, LLUIS; CERQUEIRA-COUTINHO, CRISTAL; GARCIA-FERNANDEZ, ALBA; LUIS, BEATRIZ de; BERNARDES, EMERSON S.; ALBERNAZ, MARTA S.; MISSAILIDIS, SOTIRIS; MARTINEZ-MANEZ, RAMON; SANTOS-OLIVEIRA, RALPH; ORZAEZ, MAR; SANCENON, FELIX. MUC1 aptamer-capped mesoporous silica nanoparticles for controlled drug delivery and radio-imaging applications. Nanomedicine: Nanotechnology, Biology, and Medicine, v. 13, n. 8, p. 2495-2505, 2017. DOI: 10.1016/j.nano.2017.08.006. Disponível em: http://repositorio.ipen.br/handle/123456789/28523. Acesso em: 10 May 2024.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.