Electrotransference of the mouse growth hormone gene associated with the administration of mesenchymal stem cells, in a murine model of osteogenesis imperfecta
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2019
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Journal of Cell Science & Therapy
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Osteogenesis imperfecta (OI) is an inherited connective tissue disease characterized by fragility, deformity and low bone density, as well as by other clinical manifestations. Type I OI is the mildest and most common form of
the disease, caused by mutation in the COL1A1 gene, resulting in the production of only ~50% of normal collagen.
The corresponding animal model is the oim mouse, presenting a phenotype very similar to human type I OI. We aim,
for the first time, at evaluating the electrotransference of mouse growth hormone (mGH) gene, encoding a protein
that already showed therapeutic effects, together with the administration of murine mesenchymal stem cells (MSCs),
for improving heterozygous oim mice phenotype. We already prepared and evaluated two populations of MSCs
(bone marrow and adipose tissue) that emit red fluorescence. These are administrated in different amounts and via
three routes (intravenously, intraperitoneally or locally into the femoral condyles) using the in vivo imaging system
and histological sections of femur, liver and kidneys, a methodology we recently set up and adapted to our specific
conditions. Then, we will administer the most efficient MSCs population combined with mGH gene electrotransfer.
In this bioassay, we are ready to analyse different parameters: body weight, total body, tail and femur length, bone
mineral density and femur fragility by a biomechanical flexion test. The results will indicate if the administration of
GH by gene therapy, together with MSCs infusion, can be a promising treatment for improving type I OI phenotype.
Como referenciar
GOMES, ALISSANDRA de M.; JESUS, GUSTAVO P.P. de; ZACARIAS, ENIO A.; YOSIDAKI, VANESSA L.; HIGUTI, ELIZA; RANGEL, ERIKA B.; BARTOLINI, PAOLO; PERONI, CIBELE N. Electrotransference of the mouse growth hormone gene associated with the administration of mesenchymal stem cells, in a murine model of osteogenesis imperfecta. Journal of Cell Science & Therapy, v. 10, p. 35-35, 2019. DOI: 10.4172/2157-7013-C2-053. Disponível em: http://repositorio.ipen.br/handle/123456789/30302. Acesso em: 26 Apr 2024.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.