Effects of an inhibitor of nitric oxide production on cell cycle and micronucleus frequency in irradiated human breast adenocarcinoma cells
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2019
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INTERNATIONAL NUCLEAR ATLANTIC CONFERENCE
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Breast
adenocarcinomas ar e the most frequent malignant tumor (about 25% of cases), and its
malignant outcome causes about 15% of all deaths of women with cancer. The production of
nitric oxide (NO) by isoforms of nitric ox ide synthases (NOS’s) are related to increased
malignancy a nd stimuli to metastatic progression of breast adenocarcinomas, but its presence
in irradiated cells can lead to higher frequencies of DNA damage. The work used
aminoguanidine, an inhibitor of isof orm 2 of NOS (NOS 2) to treat human breast cancer cells
(MCF 7) in non toxic concentrations (1 and 2mM) before exposure to gamma irradiation
60 Co) to assess if reduction of intracellular NO can protect from, or induce radio induced cell
damage, cell cycle disruption or death. Cells were treated and irradiated at 0, 0.5, 1, 2, 4 or
8Gy . A dministration of aminoguanidine arrest ed the cell cycle in the synthesis (S) phase,
altering the DNA repair capacity of cells. T he higher concentration (2mM) led to less
genotoxic damage (about 50%) in cells irradiated at 8Gy as obse rved using micronucleus
scoring by flow cytometry. Alternatively, 1mM of aminoguanidin e increased genotoxic
damage and induced a less significant increase of S phase cells. Despite the findings, no
significant alterations in cell proliferation rates were o bserved. Finding s showed that
aminogua ni dine can modulate radio induced ef fects on cells.
Como referenciar
AMARAL, KLEICY C.; CARVALHO, LUMA R. de; ALBIERO, ANA LIGIA; LAUBE, RAQUEL; NASCIMENTO, ANA C.G.; BONFIM, LETICIA; VIEIRA, DANIEL P. Effects of an inhibitor of nitric oxide production on cell cycle and micronucleus frequency in irradiated human breast adenocarcinoma cells. In: INTERNATIONAL NUCLEAR ATLANTIC CONFERENCE, October 21-25, 2019, Santos, SP. Proceedings... Rio de Janeiro: Associação Brasileira de Energia Nuclear, 2019. p. 1649-1658. Disponível em: http://repositorio.ipen.br/handle/123456789/30576. Acesso em: 06 Feb 2025.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.