Simultaneous intramolecular crosslinking and sterilization of papain nanoparticles by gamma radiation
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2020
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Radiation Physics and Chemistry
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Papain-based nanoparticles were recently developed using radiation technologies and proven effective to generate
nanosized crosslinked papain particles with preserved enzymatic activity. The applications of such nanostructured
systems are expected to be similar to native papain with considerable biopharmaceutical advantages
and concern drug loading among other biotechnological applications. The nature of such crosslinks and
the possibility to provide simultaneous sterilization have been hypothesized but remain not totally clarified. This
manuscript advances the discussion on the radiation-induced synthesis of protein nanoparticles by approaching
the nature of the crosslinking and the possible contribution of bityrosine linkages and disulfide bridges to the
overall nanoparticle assembly as well as the feasibility of the simultaneous sterilization process under the preestablished
conditions of processing. Papain nanoparticles were synthesized and characterized according to size,
proteolytic activity, bityrosine, cysteine content and molecular weight by SDS-PAGE upon sonication at 40 kHz.
Bacterial identification and the sterility tests were performed in accordance with ISO 11737 prior to and after
inoculating 106 CFU of Corynebacterium xerosis. Our experiments evidenced the crosslinking of rather intra- than
intermolecular nature and a contribution of cysteine bridges and bityrosine linkages to the stabilization and
formation of the papain nanoparticle assembly. The technique was effective to promote simultaneous crosslinking
and sterilization at the established conditions of processing and may be validated in accordance with the
ISO 11137.
Como referenciar
FAZOLIN, GABRIELA N.; VARCA, GUSTAVO H.C.; FREITAS, LUCAS F. de; ROKITA, BOZENA; KADLUBOWSKI, SLAWOMIR; LUGAO, ADEMAR B. Simultaneous intramolecular crosslinking and sterilization of papain nanoparticles by gamma radiation. Radiation Physics and Chemistry, v. 171, p. 1-7, 2020. DOI: 10.1016/j.radphyschem.2020.108697. Disponível em: http://repositorio.ipen.br/handle/123456789/31095. Acesso em: 26 Apr 2024.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.