Melanoma cell migration in response to red and near-infrared low-level light
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2021
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CONGRESS OF THE INTERNATIONAL UNION FOR PURE APPLIED BIOPHYSICS, 20th; ANNUAL MEETING OF THE BRAZILIAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY, 50th; CONGRESS OF BRAZILIAN BIOPHYSICS SOCIETY, 45th; BRAZILIAN SOCIETY ON NUCLEAR BIOSCIENCES CONGRESS, 13th
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Cell migration plays an important role in tissue formation and cancer progression. In vitro scratch assay has been
used for many years to study cell migration to mimic the migration of in vivo cells, and, thus, to evaluate cancer
growth. Low-level red and near-infrared light (LLL) can increase normal cell migration. However, the impact of LLL
on tumor cells remains unclear. In this work, we aimed to evaluate the effects of a single LLL dose on melanoma cell
migration. B16F10 (murine melanoma) cells were cultivated in RPMI medium with 10% of fetal bovine serum until
they reached 80% confluency. The cell line was seeded in a 6-well plate at a density of 2x10 5 cells/well in triplicate
at two different moments. A wound scratch was performed to disrupt the confluent cell monolayer with a 10 μL pipette
tip. Immediately after the injury, the cells were submitted to the LLL at two distinct wavelengths (660 and 780 nm)
provided by a LED and a laser, respectively, delivering 3 different energies (1.3, 3.6, and 6 J) at an irradiance of 4.2
mW/cm2. The control group was not irradiated. Cells were photographed immediately and at 3, 12, 24, and 36 h after
the scratch. The wound closure was measured using ImageJ software. To evaluate the overall migration, we
calculated the areas under the curve for each group. Cells exposed to the red laser at 6 J migrated slower than
control. In contrast, LLL at 780 nm promoted faster cell migration when irradiated with 3.6 J. These results suggest
that low-level LEDs at 660 nm could prevent melanoma progression in higher energies. However, 780 nm should be
avoided at middle energies.
Como referenciar
CONTATORI, CAROLINA G. de S.; PINTO, MAYARA S.; RIBEIRO, MARTHA S. Melanoma cell migration in response to red and near-infrared low-level light. In: CONGRESS OF THE INTERNATIONAL UNION FOR PURE APPLIED BIOPHYSICS, 20th; ANNUAL MEETING OF THE BRAZILIAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY, 50th; CONGRESS OF BRAZILIAN BIOPHYSICS SOCIETY, 45th; BRAZILIAN SOCIETY ON NUCLEAR BIOSCIENCES CONGRESS, 13th, October 4-8, 2021, São Paulo, SP. Abstract... São Paulo, SP: Sociedade Brasileira de Bioquímica e Biologia Molecular (SBBq), 2021. p. 249-249. Disponível em: http://repositorio.ipen.br/handle/123456789/32869. Acesso em: 08 May 2024.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.