Burn wound healing by infrared spectroscopy imaging
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2022
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INTERNATIONAL CONFERENCE ON CLINICAL SPECTROSCOPY, 12th
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Aim: Burns are one of the major causes of morbidity and the most costly traumatic injuries
worldwide1. The traditional techniques used to assess the biochemical events related to wound repair
are laborious, time-consuming and require multiple staining. Thus, the present study aims to evaluate
the feasibility of Fourier transform infrared (FTIR) spectroscopy in order to monitor the progress and
healing status of burn wounds. Methods : Third-degree burn injuries were induced on Wistar rats by
water vapor exposure. Afterwards, biopsies specimen was extracted for further histopathological
examination and IR imaging evaluation at 7 days. The spectral imaging was performed using a micro-
FTIR equipment in transflection mode (MirrIR, Kevley Technologies), with a 32 x 32 FPA of 5.5 μm
pixel size. All spectra data were baseline corrected and vector normalized. The preprocessing and
image comparison was performed using Cytospec software (Cytospec, version 2.00.5)2.
Results : The pairwise analysis was evaluated in the wavenumber region maps of 1200-1300 cm-1.
The biomolecule band associated to collagen content (1200 – 1300 cm-1) was most prominent3 on
the 7th day image in the post-burn that in healthy group.
Conclusions : Therefore, our pairwise comparison revealed that metabolic activity induced by thermal
injury increases the chemical activity associated to the healing progresses. Our findings show that
FTIR spectroscopy has potential to identify the biochemical signatures induced by burn injury.
Como referenciar
CASTRO, P.; SILVA, C.; ZEZELL, D. Burn wound healing by infrared spectroscopy imaging: a pilot study. In: INTERNATIONAL CONFERENCE ON CLINICAL SPECTROSCOPY, 12th, June 19-23, 2022, Dublin, Ireland. Abstract... Disponível em: http://repositorio.ipen.br/handle/123456789/33937. Acesso em: 14 Mar 2025.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.