Immunohistochemistry of cytokeratin proteins 10 (CK10) and 14 (CK14) demonstrates that photobiomodulation improves epithelial maturity of radiation-induced oral mucositis wounds
Carregando...
Data
2023
Data de publicação:
Autores IPEN
Orientador
Título da Revista
ISSN da Revista
Título do Volume
É parte de
É parte de
É parte de
Advances in Clinical and Experimental Medicine
Exportar
Resumo
Background. Oral mucositis (OM) is a common injury in cancer patients that can be caused by radiation
therapy to the head and neck or certain chemotherapy drugs. The clinical extent of its manifestation is variable,
especially when induced by ionizing radiation. In such cases, OM causes ulceration, severe pain when
accompanied, risk of local and systemic infections, and increased treatment costs. There is no therapeutic
consensus for the treatment of OM in recent decades.
Objectives. The focus was placed on photobiomodulation (PBM) using low-intensity lasers at red and infrared
wavelengths. This is a non-drug option that can ease discomfort and speed wound healing. The mechanism
of action of PBMs in the evolution of OM reduction is not clear.
Materials and methods. With the aim of clarifying the role of PBM in the progression of epithelial maturity
in OM ulcerations induced by ionizing radiation, the present study evaluated the effect of PBM with
a wavelength of red (λ), 660 nm and infrared, and 780 nm in radio-induced OM lesions, on the tongue of mice,
8 and 20 days after irradiation, in a single year, with 20 Gy, from a source of gamma lightning. The percentage
area corresponding to the positive staining for cytokeratin proteins 10 (CK10) and 14 (CK14) was evaluated
in the epithelial area of the lesions using the immunohistochemistry (IHC) technique, after 8 and 20 days
of lesion challenge and compared to an untreated control group. The CK10 is a protein related to epithelial
cells with a higher degree of maturity and differentiation. It can collaborate with the tissue’s innate defense
response and was significantly more expressed in the group treated with 660-nm PBM.
Discussion. The protein related to epithelial layers of lower cell maturity (CK14) is important for the resistance
of the basal area of the epithelium and should be restricted to these cell layers, closer to the connective tissue
in healthy epithelia. In this study, it did not show quantitative differences between groups. Its distribution
pattern, however, remained altered on the 20th day of the experiment for the control group. In the groups
treated with PBM, CK14 was already present with its marking delimited to the basal layer of the epithelium.
Conclusions. According to the analyses obtained in the evaluated samples, the PBM treatment was able
to optimize the expression of the epithelial proteins studied here and related to the epithelial maturation
process.
Como referenciar
SARDO, ARIANE V.N.; CORREA, LUCIANA; ZEZELL, DENISE M. Immunohistochemistry of cytokeratin proteins 10 (CK10) and 14 (CK14) demonstrates that photobiomodulation improves epithelial maturity of radiation-induced oral mucositis wounds. Advances in Clinical and Experimental Medicine, v. 32, n. Special Issue 4, p. 184-184, 2023. DOI: 10.17219/acem/2023-laserdentistry-abstractbook. Disponível em: https://repositorio.ipen.br/handle/123456789/48148. Acesso em: 06 Oct 2024.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.