Efficiency comparison between vectors containing the genomic or complementary DNA sequences of human growth hormone in an animal model of gene therapy
Carregando...
Data
Data de publicação
Orientador
Título da Revista
ISSN da Revista
Título do Volume
É parte de
É parte de
É parte de
Journal of Cell Science & Therapy
Exportar
Resumo
Our group has been working with gene therapy models for growth hormone deficiency. We are using an in vivo approach in which expression vectors containing the growth hormone (GH) gene are administered in mice,
followed by electrotransference. In previous studies, elevated levels of human GH (hGH) in mice serum (~20 ng/
mL) and high growth approximation to normal mice (catch-up growth) of ~70% for body weight and of ~80% for
femur length were obtained, using a plasmid containing the genomic sequence (gDNA) of GH with the ubiquitin-C
promoter. On the other hand, we had an indication that the complementary sequence (cDNA) may have an
advantage over gDNA in gene therapy protocols. Our objective is to carry out a comparative study between vectors
containing the hGH gDNA or cDNA sequences. First, the two vectors were analyzed for in vitro expression levels
by transfecting HEK-293 cells. Expression levels reached 250+50 ng hGH/mL for gDNA and 20+9.4 ng hGH/mL
for cDNA transfected cells. Although in vitro expression of cDNA-containing vector was lower than that containing
gDNA, we believe the cDNA vector may have better expression in vivo, due to a possible better incorporation by the
muscle cells in electrotransference. Then, bioassays will be performed administering these vectors into dwarf mice,
via electrotransference in the muscle. This will verify the expression profile of GH in vivo, concerning levels and
durability, as well as body weight, total body, tail and femur length, mouse insulin-like growth factor-1 levels and
catch-up growth.
Como referenciar
ZACARIAS, ENIO A.; GOMES, ALISSANDRA de M.; JESUS, GUSTAVO P.P. de; YOSIDAKI, VANESSA de L.; BARTOLINI, PAOLO; PERONI, CIBELE N. Efficiency comparison between vectors containing the genomic or complementary DNA sequences of human growth hormone in an animal model of gene therapy. Journal of Cell Science & Therapy, v. 10, p. 34-34, 2019. DOI: 10.4172/2157-7013-C2-053. Disponível em: http://repositorio.ipen.br/handle/123456789/30305. Acesso em: 30 Dec 2025.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.