Synthesis of paramagnetic iron oxide nanoparticles for application in in vitro three-dimensional biological models through gamma radiation and microwave reduction of iron ions

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dc.contributor.authorCORAZZA, FULVIO G.pt_BR
dc.contributor.authorPASSOS, PRISCILA de Q.S.pt_BR
dc.contributor.authorLIMA, MAYELLE M.P.pt_BR
dc.contributor.authorTOMINAGA, FLAVIO K.pt_BR
dc.contributor.authorSAKATA, SOLANGE K.pt_BR
dc.contributor.authorGONÇALVES, KARINA O.pt_BR
dc.contributor.authorCOURROL, LILIA C.pt_BR
dc.contributor.authorVIEIRA, DANIEL P.pt_BR
dc.coverageInternacionalpt_BR
dc.creator.eventoINTERNATIONAL NUCLEAR ATLANTIC CONFERENCEpt_BR
dc.date.accessioned2020-01-10T17:00:31Z
dc.date.available2020-01-10T17:00:31Z
dc.date.eventoOctober 21-25, 2019pt_BR
dc.description.abstractTwo-dimensional (2D) cell models are extensively used in biomedical research to evaluate the efficacy and safety of new drugs. However, these conventional approaches do not precisely mimic the complexity of the organ microenvironment. To overcome this obstacle, three-dimensional (3D) spheroid cell structures usually referred to as spheroids are being developed to better represent the morphological and functional similarity to the tissues. Among several techniques currently employed to produce three-dimensional cell cultures, one of the most promising is the magnetic levitation, which consists of the magnetization of the cells through adsorption of magnetic nanoparticles of iron oxide (Fe3O4), which are produced by the reaction of Fe2+ and Fe3+ ions in alkaline medium. This work produced paramagnetic iron oxide nanoparticles (PIONs) by coprecipitation from an Fe2+ source. The reduction to Fe3+ was obtained by the ionization caused by gamma radiation (60Co) at 15 or 30 kGy radiation absorbed doses. After functionalization with poly-lysine, the nanoparticle suspensions were characterized by XRD, FTIR, zeta potential analysis, DLS and TEM which showed the successful attachment of the carboxylate groups to iron, explaining the ability of the particles to be adsorbed by the membranes. Biological assays showed that these PIONs were biocompatible and efficiently could be applied to develop prostate 3D tumor spheroids model for drug screening.pt_BR
dc.event.siglaINACpt_BR
dc.format.extent2284-2295pt_BR
dc.identifier.citationCORAZZA, FULVIO G.; PASSOS, PRISCILA de Q.S.; LIMA, MAYELLE M.P.; TOMINAGA, FLAVIO K.; SAKATA, SOLANGE K.; GONÇALVES, KARINA O.; COURROL, LILIA C.; VIEIRA, DANIEL P. Synthesis of paramagnetic iron oxide nanoparticles for application in in vitro three-dimensional biological models through gamma radiation and microwave reduction of iron ions. In: INTERNATIONAL NUCLEAR ATLANTIC CONFERENCE, October 21-25, 2019, Santos, SP. <b>Proceedings...</b> Rio de Janeiro: Associação Brasileira de Energia Nuclear, 2019. p. 2284-2295. Disponível em: http://repositorio.ipen.br/handle/123456789/30623.
dc.identifier.orcid0000-0002-0007-534Xpt_BR
dc.identifier.orcid0000-0001-6072-5853pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-0007-534X
dc.identifier.orcidhttps://orcid.org/0000-0001-6072-5853
dc.identifier.urihttp://repositorio.ipen.br/handle/123456789/30623
dc.localRio de Janeiropt_BR
dc.local.eventoSantos, SPpt_BR
dc.publisherAssociação Brasileira de Energia Nuclear
dc.rightsopenAccesspt_BR
dc.subjectabsorbed radiation doses
dc.subjectbiological materials
dc.subjectcell cultures
dc.subjectcobalt 60
dc.subjectgamma radiation
dc.subjectiron ions
dc.subjectiron oxides
dc.subjectnanoparticles
dc.subjectspheroids
dc.subjectx-ray diffraction
dc.titleSynthesis of paramagnetic iron oxide nanoparticles for application in in vitro three-dimensional biological models through gamma radiation and microwave reduction of iron ionspt_BR
dc.typeTexto completo de eventopt_BR
dspace.entity.typePublication
ipen.autorPRISCILA DE QUEIROZ SOUZA PASSOS
ipen.autorFLAVIO KIYOSHI TOMINAGA
ipen.autorDANIEL PEREZ VIEIRA
ipen.autorSOLANGE KAZUMI SAKATA
ipen.autorMAYELLE MARIA PAZ LIMA
ipen.autorFULVIO GABRIEL CORAZZA
ipen.codigoautor14722
ipen.codigoautor12754
ipen.codigoautor3158
ipen.codigoautor7038
ipen.codigoautor14968
ipen.codigoautor14252
ipen.contributor.ipenauthorPRISCILA DE QUEIROZ SOUZA PASSOS
ipen.contributor.ipenauthorFLAVIO KIYOSHI TOMINAGA
ipen.contributor.ipenauthorDANIEL PEREZ VIEIRA
ipen.contributor.ipenauthorSOLANGE KAZUMI SAKATA
ipen.contributor.ipenauthorMAYELLE MARIA PAZ LIMA
ipen.contributor.ipenauthorFULVIO GABRIEL CORAZZA
ipen.date.recebimento20-01
ipen.event.datapadronizada2019pt_BR
ipen.identifier.ipendoc26272pt_BR
ipen.notas.internasProceedingspt_BR
ipen.type.genreArtigo
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sigepi.autor.atividadeVIEIRA, DANIEL P.:3158:810:Npt_BR
sigepi.autor.atividadeSAKATA, SOLANGE K.:7038:220:Npt_BR
sigepi.autor.atividadeTOMINAGA, FLAVIO K.:12754:220:Npt_BR
sigepi.autor.atividadeLIMA, MAYELLE M.P.:14968:-1:Npt_BR
sigepi.autor.atividadePASSOS, PRISCILA de Q.S.:14722:810:Npt_BR
sigepi.autor.atividadeCORAZZA, FULVIO G.:14252:810:Spt_BR
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