Evaluation of gamma-sterilization(sup(60)CO) by RT-PCR by dhfr expression detection
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2007
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INTERNATIONAL NUCLEAR ATLANTIC CONFERENCE; MEETING ON NUCLEAR APPLICATIONS, 8th/ MEETING ON REACTOR PHYSICS AND THERMAL HYDRAULICS, 15th
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The improvement of techniques to detect pathogen agents in blood had reduced significantly the contamination
mechanisms by hemocomponents in blood transfusion procedures. Ionizing radiation is a method that has
presented several applications on medicine and in currently days has been showing special attention on blood
banks which has been applied to avoid TA-GVHD development. DHFR is an enzyme constitutive in
Plasmodium protozoa and has an important role in folate metabolism on these parasites. Detecting the
expression of RNAm coder for this enzyme is possible to evaluate the viability of this parasite in blood samples.
Plasmodium chabaudi AJ is a parasite that induces lethal malaria in rodents similar to human malaria In this
work, the objective was to detect the presence of plasmodium protozoa in irradiated blood samples, infected
experimentally, through the application of a RT-PCR using primers for the coder sequence of DHFR´s mRNA.
We studied doses of ionizing radiation between 0 and 75 Gy. The irradiation procedures were accomplished in
Center of Radiation Technology of IPEN-CNEN in a
60Co panoramic source. Our results had demonstrated that
RT-PCR is a sensible method to evaluate the viability of plasmodium in blood samples because the technique
could detect low parasite burden in all tested samples.
Como referenciar
CONVERSO, ANA P.G.; VIEIRA, DANIEL P. ; ANDRADE JUNIOR, HEITOR F. de. Evaluation of gamma-sterilization(sup(60)CO) by RT-PCR by dhfr expression detection. In: INTERNATIONAL NUCLEAR ATLANTIC CONFERENCE; MEETING ON NUCLEAR APPLICATIONS, 8th/ MEETING ON REACTOR PHYSICS AND THERMAL HYDRAULICS, 15th, Sept. 30 - Oct. 5, 2007, Santos, SP. Proceedings... Disponível em: http://repositorio.ipen.br/handle/123456789/18094. Acesso em: 30 Dec 2025.
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